ÇUKUROVA ÜNİVERSİTESİ FEN BİLİMLERİ ENSTİTÜSÜ YÜKSEK ...

ABSTRACT
M.Sc. THESIS
In vitro CLONAL MIKROPROPAGATION of GF-677, AK-1 and AK-2
ALMOND x PEACH HYBRID
ROOTSTOCKS by SHOOT-TIP CULTURE
Şebnem Gözde DEMİRKÖK
DEPARTMENT OF HORTICULTURE
INSTITUTE OF NATURAL AND APPLIED SCIENCES
UNIVERSITY OF CUKUROVA
Supervisor : Prof. Dr. Ayzin KÜDEN
Year : 2006, Pages: 41
Jury : Prof. Dr. Ayzin KÜDEN
Prof. Dr. Ali KÜDEN
Doç. Dr. Mehmet Nuri NAS
This study was carried out in Biotechnology laboratory of C.U. Faculty of Agriculture,
Department of Horticulture during 2004-2006. The shoot tips of AK-1, AK-2 and GF-677 almond x
peach hybrid genotypes taken from the orchards of C.U. Faculty of Agriculture, Department of
Horticulture were used. As the experimental material the viability, shoot elongation and rooting rates
of the shoot tips were investigated .
In the experiment, MS macro and micro nutrients, vitamins and Fe-Na EDTA composition
was used. During shoot elongation and multiplication stage 0, 0.5,1, 1.5 mg/l BAP and 0.1 mg/l GA3
concentrations, during the rooting stage 0, 0.5,1, 1.5 mg/l IBA, 0.1 mg/l GA3 concentrations and MS
medium with no hormones as the control were used.
In this work, viability rate was found to be 91.25 % in AK-2, 88.75 % in AK-1 and
85 % in GF-677; multiplication rate was 2.43 shoot/explant in AK-1 genotype, 0.88 shoot/explant in
GF-677 and 0.5 shoot/explant in AK-2 genotype; rooting rate was 24.06 % in AK-1 genotype, 22.25
% in GF-677 and 6.19 % in AK-2 genotype; root number per mikroshoot was 2.78 in genotype
AK-1, 1.95 root/mikroshoot in GF-677 genotype and 0.94 root/mikroshoot in AK-2 genotype.
Key Words: GF-677, AK-1, AK-2 almond x peach hybrid rootstocks, shoot tip culture, micro
propagation
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