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Struktura chromatinu Co je to chromatin? - Laboratory of Biology of ...

Struktura chromatinu Co je to chromatin? - Laboratory of Biology of ...

Struktura chromatinu Co je to chromatin? - Laboratory of Biology of ...

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Shrnutí• Chromatin-komplex DNA a proteinů, význam pro kondenzaci DNAa regulaci genové exprese• Základní <strong>je</strong>dnotka-nukleozom, oktamer his<strong>to</strong>nů a 146bp DNA• Vyšší řády uspořádání-30nm vlákno, ostatní nejasné• Modifikace <strong><strong>chromatin</strong>u</strong>-his<strong>to</strong>nové varianty, modifikace his<strong>to</strong>nů,ATP-dependentní remodeling• Modifikace his<strong>to</strong>nů-N konce ale i his<strong>to</strong>nové kóre, acetyl, metyl,fosfát, ubiquitin• His<strong>to</strong>nový kód- soubor his<strong>to</strong>nových modifikací určujícítranskripční a jinou aktivitu oblasti• Epigenetická dědičnostLiteraturaWu J, Grunstein M: 25 years after the nucleosome model: <strong>chromatin</strong>modification. TIBS, 25, 2000.Felsenfeld G, Groudine M: <strong>Co</strong>ntrolling the double helix. Nature, Vol 421,2003Berger SL: The complex language <strong>of</strong> <strong>chromatin</strong> regulation duringtranscription, Nature, Vol 447, 2007<strong>Co</strong>sgrove MS, Boeke JD, Wolberger C: Regulated nucleosome mobility andthe his<strong>to</strong>ne code, Nature Structural & Molecular <strong>Biology</strong>, Vol 11, 2004Klose RJ, Zhang Y: Regulation <strong>of</strong> his<strong>to</strong>ne methylation bydemethylimination and demethylation, Nature Reviews Molecular Cell<strong>Biology</strong>, Vol 8, 2007

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