Estres Oxidativo en el Higado Graso Inducido por Oxitetraciclina en ...

chain breaking antioxidants. Free Rad Biol Med
1986; 2: 193-201.
[16]-Duarte J, Díaz S, Lee VE, Castro J, Velásquez
V. Hígado graso agudo del embarazo: experiencia de
8 años. Med Int Méx 2007; 23(5): 464-470.
[17] Márquez YC. Estrés oxidativo e hígado graso
inducido por etionina en ratones hembras. Trabajo
Ascenso a Prof. Asistente, Universidad Centroccidental
L.A., Barquisimeto, Venezuela, 1997; p 48-51.
[18]Halliwell B; Gutteridge J. Free radicals in biology
and medicine. 3rd ed. Oxford Science Publications.
Oxford. USA 199; p 936-940.
[19] Ohkawa H, Ohishi N, Yagi K. Assay for lipid
peroxides in animal tissues by thiobarbituric acid reaction.
Anal Biochem 1979; 95: 351-358.
[20]Wallin B, Rosengren B, Shertzer H, Camejo G.
Lipoprotein oxidation and measurement of
thiobarbituric acid reacting substances formation in
a single microtiter plate: Its use for evaluation of antioxidants.
Anal Biochem 1993; 208, 10-15.
[21]Bradford MA. A rapid and sensitive method for
the quantities of microgram of protein utilizing the
principle of protein-dye binding. Anal Biochem 1976;
72: 248-254.
[22] Fromenty B, Pessayre D. Inhibition of
mithocondrial beta-oxidation as a mechanism of
hepatotoxity. J. Pharmacol Exp Ther 1995; 67(1): 101-
154.
[23]Hansen CH, Pearson LH, Schenker S, Combes
B. Impaired secretion of triglicerides by the liver. A
cause of tetracicline-induced fatty liver. Proc Soc Ter
1963; 128 (1-2): 143-146.
[24]Labbe G, Fromenty B, Frénaux E, Morzelle V,
Lettéron P, Berson A, Pessayre D. Effects of varius
tetracycline derivatives on in vitro and in vivo -oxidation
of fatty acids, egress of triglicerides from the
A B C
Ruiz de Rivero M.D., Mendoza C.A. y López-Ortega A.A.
85
liver, accumulation of hepatic triglycerides, and mortality
in mice. Biochem Pharmacol 1991; 41(4): 638-
641.
[25]Lettéron P, Fromenty B, Terri B, Degott C,
Pessayre D. Acute and chronic hepatic steatosis lead
to in vivo lipid peroxidation in mice. J Hepatol 1996;
24: 200-208.
[26] Petrenko M. A new look at the mechanism of
action of antibiotics of the tetracycline series. Tetracyclines
as components of radical-generating systems.
Antibiot Khimioter 1994; 39(7): 10-14.
[27]López-Ortega A, Mendoza C, Ferraro S,
Márquez YC. Producción de dienos conjugados en el
hígado graso experimental del ratón. Acta Cient Venez
1997; 48 (supl. 1): 90.
[28]Jaeschke H, Gores GJ, Cederbaum A, Hinson
JA, Pessayre D, Lemasters JJ. Mechanisms of Hepatotoxicity.
Toxicol Sc 2002; 65: 166-176.
[29]Amacher DE, Martin BA.Tetracycline-induced
steatosis in primary canine hepatocyte cultures.
Fundam Applied Toxicol 1997; 40: 256-263.
[30]Lettéron P, Sutton A, Mansouri A, Fromenty B,
Pessayre D. Inhibition of microsomal triglyceride transfer
protein: Another mechanism for drug-induced steatosis
in mice. Hepatology 2003; 38(1): 133-140.
[31]Spann NJ, Kang S, Li AC, Chen AZ, Newberry
EP, Davidson NO, Hui ST, Davis RA. Coordinate transcriptional
repression of liver fatty acid-binding protein
and microsomal triglyceride transfer protein blocks
hepatic very low density lipoprotein secretion without
hepatosteatosis. J Biol Chem 2006; 281(44): 33066-
33077.
[32]Yu HY, Wang BL, Zhao J, Yao XM, Gu Y, Li Y.
Protective effect of bicyclol on tetracycline-induced
fatty liver in mice. Toxicology 2009; 261(3): 112-118.
Figura 1.- Observación macroscópica de hígados: 1A-Control 1B-Dosis Alta de OT. Fotomicrografía de hígados:
1C-Control (40x H&E) 1D-Dosis Alta de OT (50x H&E).
D