Novocastratm Ready-to-Use Mouse Monoclonal Antibody E-Cadherin

More documents

Recommendations

Info

RTU-E-Cad Page 1
NovocastraTM Ready-to-Use Mouse Monoclonal Antibody E-Cadherin Product Code: RTU-E-Cad Intended Use For in vitro diagnostic use. RTU-E-Cad is intended for the qualitative identification by light microscopy of E-Cadherin molecules in paraffin sections. The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient’s clinical history and other diagnostic tests by a qualified pathologist. Principle of Procedure Immunohistochemical (IHC) staining techniques allow for the visualization of antigens via the sequential application of a specific antibody to the antigen (primary antibody), a secondary antibody to the primary antibody and an enzyme complex with a chromogenic substrate with interposed washing steps. The enzymatic activation of the chromogen results in a visible reaction product at the antigen site. The specimen may then be counterstained and coverslipped. Results are interpreted using a light microscope and aid in the differential diagnosis of pathophysiological processes, which may or may not be associated with a particular antigen. Clone 36B5 Immunogen Prokaryotic recombinant protein corresponding to the N-terminal external region of the E-Cadherin molecule. Specificity Human E-Cadherin. Reagent Composition RTU-E-Cad is a ready to use liquid tissue culture supernatant, presented in 5% horse serum in PBS containing 12 mM sodium azide as a preservative. Ig class IgG1 Total Protein Total Protein Concentration Range 1.0–8.0 g/L. Refer to vial label for lot specific total protein concentration. Antibody Concentration Greater than or equal to 5.4 mg/L as determined by ELISA. Refer to vial label for batch specific Ig concentration. Recommendations On Use Immunohistochemistry (see D. Methodology) on paraffin sections. Incubate tissue section with primary reagent for 15 minutes at 25 o C. High temperature antigen retrieval using 0.01 M citrate retrieval solution (pH 6.0) is recommended. This antibody is pre-titred for use and does not require further dilution when used with the secondary detection system, RE7100-K. Storage and Stability Store at 2–8 o C. Do not freeze. Return to 2–8 o C immediately after use. Do not use after expiration date indicated on the vial label. Storage conditions other than those specified above must be verified by the user. Specimen Preparation The recommended fixative is 10% neutral-buffered formalin for paraffin-embedded tissue sections. Warnings and Precautions This reagent has been prepared from the supernatant of cell culture. As it is a biological product, reasonable care should be taken when handling it. The molarity of sodium azide in this reagent is 12 mM. A Material Safety Data Sheet (MSDS) is available upon request for sodium azide. Consult federal, state or local regulations for disposal of any potentially toxic components. Specimens, before and after fixation, and all materials exposed to them, should be handled as if capable of transmitting infection and disposed of with proper precautions. 1 Never pipette reagents by mouth and avoid contacting the skin and mucous membranes with reagents and specimens. If reagents or specimens come in contact with sensitive areas, wash with copious amounts of water. Seek medical advice. Minimize microbial contamination of reagents or an increase in non-specific staining may occur. Incubation times or temperatures, other than those specified, may give erroneous results. Any such changes must be validated by the user. Quality Control Differences in tissue processing and technical procedures in the user’s laboratory may produce significant variability in results, necessitating regular performance of in-house controls in addition to the following procedures. Controls should be fresh autopsy/biopsy/surgical specimens, formalin-fixed, processed and paraffin wax-embedded as soon as possible in the same manner as the patient sample(s). RTU-E-Cad Page 2
Page 1: Novocastra TM Ready-to-Use Mouse Mo
Page 5 and 6: 5. Wijnhoven BP, Dinjens WN and Pig
Page 7 and 8: Novocastra TM Anticorps Monoclonal
Page 9 and 10: Limites Générales L’immunohisto
Page 11 and 12: 16. Rincer les lames à l’eau. 17
Page 13 and 14: Controllo Qualità Differenze nella
Page 15 and 16: Metodologia immunoistochimica per l
Page 17 and 18: Novocastra TM Gebrauchsfertiger liq
Page 19 and 20: Allgemeine Beschränkungen Die Immu
Page 21 and 22: 16. Die Objektträger mit Wasser ab
Page 23 and 24: Reduzca al mínimo la contaminació
Page 25 and 26: Metodología inmunohistocitoquímic
Page 27 and 28: Novocastra TM Anticorpo Monoclonal
Page 29 and 30: A coloração de tecidos depende do
Page 31 and 32: 18. Contrastar com hematoxilina. 18
Page 33 and 34: Kvalitetskontroll Skillnader i väv
Page 35 and 36: Immunhistokemisk metodologi för an
Page 37 and 38: Novocastra TM Έτοιμο για
Page 39 and 40: Τα αντισώματα που π
Page 41 and 42: 15. Επωάστε τις αντι
Page 43 and 44: Kvalitetskontrol Forskelle i behand
Page 45 and 46: Metodik for immunohistokemi ved anv
Page 47 and 48: RTU-E-Cad Page 46

Novocastratm Ready-to-Use Mouse Monoclonal Antibody E-Cadherin

Create successful ePaper yourself

Delete template?

Save as template?