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4. % G/C: 40-60%. (Tm)5. Secuencias complementarias. Evitar:a) Homología INTRAprimer (hasta 3pb): hairpinsb) Homología ENTRE primer, sobre todo en 3’ (dímeros)c) Secuencias polinucleotídicas (>4)6. Terminal 3’a. GC clamp: última base G o C y hasta 2 G/C en últimas 5pb
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