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Rehm, G. Reed, A PuÈ hler, P. Stadler, eds., Biotechnology -A Multi-Volume ComprehensiveH.J.2 nd ed. Wiley-VCH, ISBN 1-56081-602-3.TreatiseB. Atkinson and F. Maviura (1991). Biochemical Engineering and Biotechnology Handbook. 2 ndBlack (1999) Microbiology -Principles and Explorations, 4 th ed., Prentice Hall. ISBN 0-13-J.920711-2.; kl-GdQ9GReferencesOvtvyOv}vAvfG,vpGPv${v+vyOvyG)vzvfOvWvI{vF9v$#v|J9v|(vzvgv}vyGBv+vtvAvSG#v|,>vAvvJ9vgvFGQv|hJ9v*QvWv!h?v*QhO?vp9v\E)vyEJGQ9vWvAvS:G.?v+vZvMvWvyG,9v%v!Ch9v%v@QvDvwvyd9vJvAvSG9v"v+vzvffC9v%v=vTv"v!)vyE9v$QO9vZv|hC9v%v\Qvgv!9%A+zw

Watson, M. Gilman, J. Witkowski, M. Zoller (1992) Recombinant DNA, 2 nd ed. Scientific AmericanJ.Books, ISBN 0-7167-2282-8.Saarela, G. Mogensen, R. Fonden, J. Matto, T. Mattila-Sandholm (2000). «Probiotic bacteria:M.Functional and Technological Propelties.» Journal of Biotechnology, vol. 84, p. 197.Safety,Forde, G. F. Fitzgerald (2000). «Biotechnological Approaches to the Understanding and ImprovementA.of Mature Cheese Flavour.» Current Opinion in Biotechnology, 484.B. Archer (2000), «Filamentous Fungi as Microbial Cell Factories for Food Use.» Current OpinionD.in Biotechnology, 478.Fleming, K. Kyung, F. Breidt (1995).Vegetable Fermentations in Biotechnology 2 nd ed. 629.H.9, G. Reed, T. Nagodawithana (ed.). VCR-Wiley, ISBN 3527-28319-6.Vol.Shaver, K Batajoo (1995).Fermented Feeds and Feed Products in Biotechnology 2 nd Edition, 769.R.9, G. Reed, T. Nagodawithana (ed.). VCR-Wiley; ISBN 3~527.Vol.T. Brown (2001) Gene Cloning and DNA Analysis: An Introduction, 4 th ed. Blackwell Science Inc,ISBN 0-9320-5901-X.T. Brown (1999).Genomes. John Wiley & Sons, ISBN 1-85996-201-7.B. Dixon (1996). Power Unseen: How Microbes Rule the World, W. H. Freeman, ISBN 07167-4550-XA. Demain, JDavies (1999) Industrial Microbiology and Biotechnology. 2 nd ed. ASM Press, ISBN1-55581-128-0.B. R. Glick, J. J. Pasternak (1998) Molecular Biotechnology: Principles and Applications of RecombinatDNA. 2 nd ed. Amer. Society for Microbiology, ISBN 1-55581136-1.A. L. Lehninger, D.L. Nelson, M.M. Cox (2000).Principles of Biochemistry. 3rd ed., Worth Publishing,ISBN 1-5725-9931-6.J. Lengeler, G. Drews, H. Schlegel (1999). Biology of the Prokaryotes Thieme, 3-13108411-1P. Prave, U. Faust, W. Sittig, D. Sukatsch (1987).Fundametals ofBiotechnology, VCH Publishing,ISBN 0-8957-3224-6.L. Stryer (1995). Biochemistry, 4 th ed. W. H. Freeman and Co., ISBN 0-71673687-X G Walsh, DHeadon (1994). Protein Biotechnology. John Wiley & Sons, ISBN 0-471944396OP Ward(1989).Fermentation Biotechnology: Principles; Processes, and Products, Prentice Hall, ASIN0-1331-5052-6.Q}MCGe9gayGFermented foodL. Beuchat (1995) Indigenous Fermented Foods in Biotechnology. 2 nd Edition, 505. Vol. 9, GReed,T Nagodawithana (ed.). VCR-Wiley, ISBN 3-527-28319-6.N. Olson (1995) Cheese in Biotechnology. 2 nd Edition, 353. Vol. 9, GReed, T Nagodawithana (ed.).VCR-Wiley, ISBN 3527-28319-6.G. Burkhalter, C. Steffen, Z. Puhan (1986).Cheese, Processed Cheese, and Whey in Ullmann's Encyclopediaof IndustrialChemistry. 5 th edition A6, 163. Wiley-VCR, ISBN 3-527-20106-8.398

Ross, S. Morgan, C. Rill (2002). «Preservation and Fermentation: Past, Present and future.»R.P.Journal of Food Microbiology: vol. 79, p. 3.InternationalTeuber (1993) Lactic Acid Bacteria in Biotechnology Second, Completely Revised Edition, 325.M.1, R.Sahm (ed.). VCR Verlagsgesellschaft, ISBN 3527-28337-4.Vol.Galbe, G. Zacchi (2002). «A Review of the Production of Ethanol from Softwood.» AppliedM.and Biotechnology: vol. 59, p. 618.MicrobiologyE. Wheals, L.C. Basso, D. M. Alves, H. V. Amorim (1999), «Fuel Ethanol after 25 years.»A.in Biotechnology: vol. 17, 482TrendsGong, N.J. Cao, J. Du, G.T. Tsao (1999). «Ethanol Production from Renewable Resources.»C.S.in Biochemical Engineering/Biotechnology: vol. 65, p. 207.AdvancesSenn, R. Pieper (1996) Ethanol -Classical Methods in Biotechnology. 2 nd Edition, 5. Vol. 6, MT.(ed.). VCR-Wiley, ISBN 3-527-28316-1RoehrLogsdon (1994). Ethanol in Kirk-Othmer. Encyclopedia of Chemical Technology. 4 th Edition 9,J.Interscience-Wiley, ISBN 0-471-52677-0812.Maiorella, R. Blanch, C. Wilke (1984). «Biotechnology Report: Economic Evaluation ofAlternativeB.Ethanol Fermentation Processes.»Biotechnology and Bioengineering: vol. 26, p.1003.Durre, R. Bahl (1996). Microbial Production of Acetone/Isopropanol in Biotechnology. 2 nd Edition.P.229. Vol. 6, M. Roehr (ed.). VCH-Wiley, ISBN 3-527-28316-1Durre, R. Fischer, A. Kuhn, K. Lorenz, W. Schreiber, B. Sturzenhofecker, S. Ullmann, K. Winzer,P.U. Sauer (1995), «Solventogenic Enzymes of Clostridium Acetobutylicum: CatalyticGenetic Organization, and Transcriptional Regulation.» FEMS Microbiology:Properties,17, p. 251.vol.Food and lactic acid fermentatione9gayGQ}GEthanold(!9D*M. J. Davies (2001). «Corn-to-car» Ethanol Production.» Trends in Biotechnology: vol. 19, p. 380.Kosaric (1996) Ethanol -Potential Source of Energy and Chemical Products in Biotechnology. 2 ndN.121. Vol. 6, M. Roehr (ed.). VCR-Wiley, ISBN 3-52728316-1Edition,l-Butanol, acetonef(A+S}Ghd(!9C(+{BG/{BGArnold, T. Becker, A. Delgado, F. Emde, A. Enenkel (2002). «Optimizing High Strength AceticS.Bioprocess by Cognitive Methods in an Unsteady State Cultivation.» Journal of Bio-Acidtechnology: vol. 97, 133.399

Ebner, H. Follmann, S. Sellmer (1995) Vinegar in Biotechnology. 2 nd Edition, 579. Vol. 9, G.H.T. Nagodawithana (ed.). VCH-Wiley, ISBN 3-527-28319-6Reed,Karaffa, E. Sandor, Fekete, A Szentirmai(2001). «The biochemistry of Citric Acid AccumulationL.by Aspergillus Niger.» Acta Microbiologica et Immunologica Hungarica: vol. 48, p. 429.Roehr, C. Kubicek, J. Kominec (1996) Citric Acid in Biotechnology 2 nd Edition, 307. Vol. 6, MM.(ed.). VCH-Wiley, ISBN 3-527-28316-1RoehrVerhoff (1986) Citric Acid in Ullimann's Encyclopedia of Industrial Chemistry. 5 th edition A7,F.Wiley-VCH, ISBN 3-527-20107-6103.Roehr, C. Kubicek, J. Kominek (1996) Gluconic Acid in Biotechnology 2 nd Edition, 347. Vol. 61M.Roehr (ed.). VCHMRoehr, C. Kubicek. J. Kominek (1996). Further Organic Acids in Biotechnology 2 nd Edition,M.Vol. 6, M Roehr (ed.). VCH-Wiley, ISBN 3-527-28316-1363.H. Ebner, S. Sellmer, H. Follmann (1996).Acetic Acid in Biotechnology. 2 nd Edition, 381. Vol. 6, MRoehr (ed.). VCH-Wiley, ISBN 3-527-28316-1.H. Ebner, H. Follmann, S. Sellmer (1996) Vinegar in UIlmann's Encyclopedia of Industrial Chemistry.5 th edition A27, 403. Wiley-VCH, ISBN 3-527-20127-0Citric acidf(}+zyG^>G. Blair, P. Staal (1993) Citric Add in Kirk-Othmer Encyclopedia of Chemical Technology 4 th Edition6, 354. Interscience-Wiley, ISBN 0-471-52674-6R. Datta (1995).Hydroxycarboxylic Acids in Kirk-Othmer Encyclopedia of Chemical Technology.4 th Edition 13, 1042. Interscience-Wiley, ISBN 0-471-52682-7S. Chahal (1990) Lactic Acid in Ullmann's Encyclopedia of Industrial Chemistry. 5 th edition A15,97. Wiley-VCH, ISBN 3-527-20115-7H. Hustede, H-J. Haberstroh, E. Schinzing (1989).Gluconic Acid in UIlmann's Encyclopedia of IndustrialChemistry. 5 th edition A12, 449. Wiley-VCH, ISBN 3527-20112-2Amino acids?+"+|}GV9>}GW. Leuchtenberger (1996).Amino Adds Technical Production and Use in Biotechnology. 2 nd Edition,465. Vol. 6, M. Roehr (ed.). VCH-Wiley, ISBN 3-52728316-1N. Esaki, S. Nakamori, T. KUrhara, S. Furuyoshi, K. Soda (1996). «Enzymology ofAmino AcidProduction,» in: Biotechnology. 2 nd Edition, 503. Vol. 6, M. Roehr (ed.).VCH-Wiley, ISBN 3-527-28316-1K. Araki (1992).Amino Acids,Survey, in: Kirk-Othmer Encyclopedia of Chemical Technology. 4 thEdition 2, 504. Interscience-Wiley, ISBN 0-471-52669-XA. Kleemann, W. Leuchtenberger, B. Hoppe, H. Tanner (1985).Amino Acids in lTIlmann's Encyclopediaof Industrial Chemistry. 5 th edition A2, 57. Wiley-VCR; ISBN 3-527-20102-5400

G. Peters-Wendisch, B. Schiel. V. F. Wendisch, E. Katsoulidis, B. Mockel, H. Sahm, B. J. EikmannsP.(2001) «Pyruvate carboxylase is a major bottleneck for glutamate and lysine produc-by Corynebacterium glutamicum.» Journal of Molecular Microbiology andtionvol. 3, 295Biotechnology:Kawakita (1992). «L-Monosodium Glutamate.» In: Kirk-Othmer Encyclopedia of ChemicalT.4 th Edition 2, 571.Interscience-Wiley. ISBN 0-471-52669XTechnology.Kawakita, C. Sano, S. Shioya. M. Takehara, S. Yamaguchi (1990). Monosodium Glutamate inT.Encyclopedia of Industrial Chemistry. 5 th edition A16. 711. WileycVCH, ISBN 3-Ullmann'sOhnishi. S. Mitsuhashi. M. Hayashi. S. Ando. H. Yokoi. K. Ochiai. M. Ikeda (2002). «A novelJ.employing Corynebacterium glutamicum genome information to generate amethodologyA. de Graaf, L. Eggelin, H. Sahm (2001).» Metabolic engineering for L-Iysine production byCorynebacteriumA.glutamicum.»Advances in Biochemical Engineering/Biotechnology: vol. 73, p. 9.t-phenylalanine,Aspartame,t-aspartic acidandKuhn, P. Durrschmidt, J. Mansfeld, R.Ulbrich-Hofmann (2002). «Boilysin and thermolysin in dipeptideD.synthesis: acomparative study.» Biotechnology and Applied Biochemistry: vol. 36, p. 71.Bommarjus, M. Schwarm, K. Drauz (2001). «Comparison of Different Chemoenzymatic ProcessA.Routes to Enantiomerically Pure Amino Acids.» Chimia vol. 55, p. 50.Griengl, H. Schwab, M. Fechter (2000). «The synthesis of chiral cyanohydrins by oxynitrilases.»TrendsH.in Biotechnology: vol. 18, p.252.occurrence, application,Antibiotics:of actionmechanismD. Borders (1992) Antibiotics, Survey in: KirkOthmer Encyclopedia of Chemical Technology 4 thCauwenbergh (1992) AntiparaSitic Agents, Antimycotics in Kirk-Othmer Encyclopedia. of ChemicalG.Technology 4 th Edition 3, 473. Intersciel1ce-Wiley, ISBN 0471-52671-1Plempel, H. Bbshagen, J. McGuire (1985). Antimycotics in Ullmann's Encyclopedia ofIndustrialM.Chemistry. 5 th edition A3 77. Wiley-VCH, ISBN 3-527-20103-3x+|9@(zjyGL-Glutamic acid^>-L52720116-5Methionine, L-Iysine, L-threonine,#+!(+D+|L,#+!(*QDyGh,L-_T*;yGnew Llysine-producing mutant.» Applied Microbiology and Biotechnology: vol. 58, p. 217.LL-x+@Q9=S}G^>h{+"qyG -#+!:G ,e9@Q9=S}GJ. Fry, The world market for intense sweeteners (1999). World RevNutr Diet. 85, p. 201T. Sato, T. Tosa T.Production of L-aspartic acid (1993). Bioprocess Technol. Vol. 16, 15Amino acids via enzymatic transformation?+}*R!}G J;*(JAyG?+"+|CV9>C?aSG(

Ohno, M Otsuka. M. Yagisawa, S. Kondo H. Oppinger, H. Hoffmann, D. Sukatsch, L Hepner.MMale (1985) Antibiotics in Ullmann's Encyclopedia of Industrial Chemistry. 5 th edition A2,CDessen, A. M. Di Guilmi, T. Vernet, O.Dideberg (2001).»Molecular mechanisms of antibioticA.in gram-positive pathogens.» Curr Drug Targets Infect Disord. Vol. 1, p. 63.resistanceChater, M. Bibb (1997) Regulatian of Bacterial Antibiotic Production in Biotechnology2nd Edition,K.57. Vol. 7. Hv. Dbhren H. Kleinkauf (ed.). VCH-Wiley, ISBN 3 527-28310-2DoÈ hren, U GraÈ fe (1997) General Aspects of Secondary Metabolism in Biotechnology 2 nd Edition,Hv1. VoL 7; Hv. DoÈ hren.Moreillon and J. M. Entenza(2001). «Antibiotic resistance: learning from animal feeds and animalP.experimentation.»Clinical Microbiology and Infection, 7 Suppl 5, p. 13.F.Martin (1998). «New aspects of genes and enzymes for beta-Iactam antibiotic biosynthesis.»J.Microbiology and Biotechnology: vol.50, p. 1AppliedLindner, D. Bonner, W. Koster (1992) Monobactams in Kirk-Othmer Encyclopedia of ChemicalK.Technology. 4 th Edition 3, 107. Interscience-Wiley, ISBN 0471-52671-1Roberts (1992) Cephalosponns in Kirk Othmer Encyclopedia of Chemical Technology. 4 th EditionJ.3, 28. Interscience-Wiley, ISBN 0471-52671-1Southgate, N. Osborne (1992) Carbapenems and Penems in Kirk-Othmer Encyclopedia of ChemicalR.Technology 4 th Edition 3, 1,. Interscience-Wiley, ISBN 0-471-52671-1A. Wegman, M.B.A Janssen, F. van Rant-wijk, R.A. Sheldon (2001), «Towards Bio-catalytic-M.of aà -Lactam Antibiotics.»Advanced Synthesis and Catalysis: vol. 343, p. 559Synthesisde Sandt, E. de Vroom (2000), «Innovations in cepahalosporin and penicillin production:Evanthe antibiotics industry green.» Chimica OGGI, 72.paintingA. Penalva, R. T. Rowlands, G. Turner(1998), The optimization of penicillin biosynthesis inM.Trends in Biotechnology: vol. 16, p. 483,fungiKleinkauf, Hv. DoÈ hren (1997).PeptideAntibiotics in Biotechnology 2 nd Edition, 277. Vol. 7, Hv.HH. Klein- kauf (ed.), VCH-Wiley, ISBN 3-52728310-2.Dohren,467. Wiley. VCH, ISBN 3-527-20102-5.,?*(+AG G L9A! ,,f9"ZyG?|h9tCGJGO9]|Antibiotics: industrial production resistanceH. Kleinkauf (ed.). VCH-Wiley, ISBN 3Diet527-28310-2e9Av:9A+

polyetherGlycopeptide,nucleoside antibioticsandiQLCchinones and other aromatic antibioticsTetracyclines,K. Dornberger, H. Salz (1997) Biotechnical Drugs as Antitumor Agents in Biotechnology.U.Grafe, nd Edition, 641. Vol. 7, Hv. Dohren, H. Kleinkauf (ed.). VCH-Wiley, ISBN 3-527-28310-22H. Kirst (2001) Antibiotics, Macrolides in Kirk-Othmer Encyclopedia of Chemical Technology 4 thJGO9]|?*(+AG#|JGO+A==yG?*QwTyG[(=yGh,QD*EJGO*GR(+zw+"yGhA. Srinivasan, J. D. Dick, T. M. Perl (2002).»Van-comycin resistance in staphylococci,»ClinicalMicrobiology Reviews: vol. 15, p. 430.J. Kallen, V. Mikol, V. Quesniaux, M. Walkinshaw, E. Schneider-Scherzer, K. Schorgendorfer, G.Weber, H. Fliri (1997).Cyclosporins: Recent Developments in Biosynthesis, Pharmacology andBiology, and Clinicla Applications in Biotechnology 2 nd Edition, 535. Vol. 7, Hv. DoÈ hren,H.Kleinkauf (ed.), VCH-Wiley, ISBN 3-527-28310-2?+"+|}GJGO*R(w*;jyG #|?*(+AGJGO9]|Aminoglycoside antibioticsW. Piepersberg, J. Distler (1997) AminoglycoComsides and Sugar Components in Other SecondaryMetabolites in Biotechnology 2 nd Edition, 397. Vol. 7, Hv D6hren, H Kleinkauf (ed.). VCH-Wiley, ISBN 3-527-28310-2.?*QafJGO9]|h,f(y("+WyGhf("+WyGh,#+zw+SGQAAyG #|?*(+AGJGO9]|J. Hlavka, G. Ellestad, I. Chopra (1992) Tetracyclines in Kirk-Othmer Encyclopediaof ChemicalTechnology 4 th Edition 3,331. Interscience-Wiley, ISBN 047152671-1T. Nagabhushan, G. Miller, K. Vanna (1992) Chloramphenicol and Analogues in Kirk Othmer Encyclopediaof Chemical Technology 4 th Edition 2, 961. Inter science-Wiley, ISBN 0-471-52669-XO*:hQv9CG #|?*(+AGJGO9]|Macrolide antibioticsEdition 3; 169. Intersdence-Wiley, ISBN 0471-52671-1P. Zhong, V. Shortridge (2001). «The emerging new generation of antibiotic: ketolides.» Curr DrugTargets Infect Disord. Vol. l, p. 125.?*(+AGIO*OFJGO9]|JGO9]CNew pathways to antibioticsR. Gokhale, D. Tuteja (2001). Biochemistry of Polyketide Synthases in Biotechnology 2 nd Edition,341. Vol. 10, H. Rehm(ed.). VCH-Wiley, ISBN 3-527-28320-XY. Xue, D. H. Sherman (2001). «Biosynthesis and combinatorial biosynthesis of pikromycin-relatedmacrolides in Strepto-myces venezuelae.»Metabolic Engineering: vol. 3, p. 15.M. Chaltrain, P. M. Salmon, D. K. Robinson, B. C. Buckland (2000). «Metabolic engineering anddirected evolution for the production of pharmaceuticals.» Current Opinion in Biotechnology:vol. 11, p. 209.403

D. Hancock, R. Viola (2002), «Biotechnological approaches for L-ascorbicacid production,»-R.Biotechnol: vol. 20, p. 299.TrendsKuellmer (2001) Ascorbic Acid in Kirk-Othmer Encyclopedia ofChemical Technology. 4 th EditionV.25, 17. Inter-science-Wiley, ISBN 0-471-52694-0.Scott (1998)Vitamins, Survey in Kirk-Othmer Encyclopedia ofChemical Technology. 4 th Edition25,1.J.Interscience-Wiley, ISBN 0-471-52694-0.(1998) Riboflavin(B2) in: Kirk-Othmer Encyclopedia ofChemical technology. 4 th EditionF.Yoneda132. Inter-science-Wiley, ISBN 0-471-52694-0.25,Scott (1998) Vitamin B12 in Kirk-Othmer, Encyclopedia ofChemical Technology. 4 th Edition 25,J.Interscience-Wiley, ISBN 0-471-52694-0.193.Eggersdorfer, G. Adam, M. John, W. Hahnlein, L. Labler, K-U. Baldenius, L. Bussche-Hunnefeld,M.E.Hilgemann, P. Hoppe, R. Sturmer, F. Weber, A. Ruttimann, G. Moine, H-P. Hoh-R. Kurth, J. Paust, H. Pauling, B-J. Weimann, B. Kaesler, B. Oster, U. Fechtel, K.mann,B. Potzolli, M. Casutt, T. Koppe, M. Schwarz. U. Hengartner, A. Saizieu, C. Wehrli,Kaiser,Blum (1996) Vitamins in Ullmann's Encyclopedia ofIndustrial Chemistry. 5 th edition A27,R.443. Wiley-VCH, ISBN 3-527-20127-0.p.Kuninaka (1996) Nucleotides and Related Compounds in Biotechnology 2 nd Edition, 561. vol. 6.A.Roehr (ed.). VCH-Wiley, ISBN 3-527-28316-1.M.Suhadolnik, N. Reichenbach (1992) Nucleosidesand Nucleotides in: Kirk-Othmer EncyclopediaR.Chemical Technology 4 th Edition 3, p. 214. Interscience-Wiley, ISBN 0-471-52671-1.ofMakkar, S.S. Cameotra (2002). «An update on the use of unconventional substrates for biosurfactantR.S.production and their new applications.»Applied Microbiology and Biotechnology,Ron. E. Rosenberg (2001). «Natural roles of biosurfactants Environ.» Microbiology, vol. 3, p.E.Z.229.Banat, R.S. Makkar, S.S. Cameotra (2000), Potential commercial applications of microbialI.M.Applied Microbiology and Biotechnology, vol. 53, p. 495.surfactants.Kosaric (1996) Biosurfactants in Biotechnology. 2 nd Edition, 659. Vol. 6, M. Roehr (ed.). VCH-N.ISBN 3-527 28316-1.Wiley,Ochsner, T. Hembach, A. Fiechter(1996), «Production ofrhamnolipid biosurfactants.»AdvancesU.A.in Biochemical Engineering/Biotechnology: vol. 53, p. 89.J9"+|9A+qyGVitaminsS. Shimizu(2001) Vitamins and Related Compounds: Microbial Production inBiotechnology. 2 ndEdition, 319. vol. 10, H. Rehm (ed.). VCH-Wiley, ISBN 3-527 - 28320-X.JGO+@(+zv(+"yGhJGO*R(+zv(+"yGNucloside and nucleotidesQ@(AyG ,JaTyGJGQ]JAT|h{+}GAyGJ9]qJBiosurfactant and biocosmeticvol.58, p. 428.404

van Kranenburg, I.C. Boels, M.Kleerebezem, W.M.de Vos(1999), «Genetics andengineering ofR.exopolysac-charides for food: approaches for theproduction of existing and novelmicrobial(1998), Novel and established applications of microbial polysaccharides TIBTech 16,I.Sutherland41. p.Becker, F. Katzen, A. Puhler, L. lelpi (1998) «Xanthan gum biosynthesis and application: a biochemical/geneticA.perspective.»Applied Microbiology and Biotechnology, vol 50, p.145.Sutherland (1996) Extracellular Polysaccharides in Biotechnology 2 nd Edition,613. Vol. 6, MI.(ed.). VCH-Wiley, ISBN 3-527-28316-1RoehrCote, J. Ahlgren (1995) Microbial Polysaccharides in Kirk-Othmer Encyclopedia of ChemicalG.4 th Edition 16, 578. Interscience-Wiley, ISBN 0471-52685-1TechnologySteinbuchel, S. Hein (2001), «Biochemical and molecular basis of microbial synthesis of polyhydroxyalkanoatesA.in microorganisms.» Advances in Biochemical Engineering/Biotechnology:B. Hinman, J. A. Jones, R. V. Lewis (2000), «Synthetic spider silk: a modular fiber.» Trends inM.vol. 18, p.374.Biotechnology:Y. Galaev, B. Mattiasson (1999), ««Smart» polymers and what they could do in biotechnologyI.medicine.» Trends in Biotechnology: vol. 17, p. 335.andOesterhelt (1998), «The structure and mechanism of the family of retinal proteins from halophilicD.archaea.» Current Opinion in Structural Biology: vol. 8, p. 489.Braunegg, G. Lefebvre, K. F. Genser (1998). «Polyhydroxyalkanoates. biopolyesters from renewableG.resources:physiological and engineering aspects.» Journal of Biotechnology: vol.65,Fahnestock and L. Bedzyk (1997). «Production of synthetic spider dragline silk protein in PichiaS.Applied Microbiology and Biotechnology: vol. 47, p. 33.pastoris.»chel (1996). PHB and Other Polyhydroxyalkanoic Adds in Biotechnology 2 nd Edition.A.SteinbuÈVol. 6, M. Roehr (ed.). VCR-Wiley. ISBN 3-527-28316-1403.Salerno and I. Goldberg (1993). «Cloning, expression and characterization ofa synthetic analogA.the bioadhesive precorsor protein of the sea mussel Mytilus edulis.»Applied MicrobiologytoSchrader and R. Berger (2001).Biotechnological Production of Terpenoid Flavor and FragranceJ.in Biotechnology. 2 nd Edition. 373. Vol. 10, H. Rehm (ed.). VCH-Wiley, ISBN 3-CompoundsJGO*OfO*Q9wTyG?+

(2000) Industrial Biotranformations in Biotechnology 2 nd Edition 277. Vol. 8b. DU.Bornscheuer(ed.). VCR-Wiley, ISBN 3-527-28324-2KellyFaber (2000).Biotransfonnations in Organic Chemistry. 4 th ed., SpringerVerlag, ISBN 3-540-K.66334-7.Liese, K. Seelbach, C. Wandrey (2000).Industrial Biotransfonnations. Wiley-VCR, ISBN 3-527-A.30094-5.Rabenhorst (2000).Biotechnological Production of Natural Aroma Chemicals by FermentationJ.in Biotechnology 2 nd Edition, 333. Vol. 8b, D. Kelly (ed.). VCR-Wiley, ISBN 3-ProcessesSchulze, M. G. Wubbolts (1999), «Biocatalysis for industrial production of fine chemicals.»B.Opinion in Biotechnology: vol 10, p. 609.Current(1999). Biotransfonnations -Practical Aspects in Biotechnology 2 nd Edition, 25. Vol. 8a, D.D.Kelly(ed.). VCR-Wiley, ISBN 3-527-30104-6KellyTurner (1997).Perspectives in Biotransformation in Biotechnology 2 nd Edition, 5. Vol. 8a, DM.(ed.). VCR-Wiley, ISBN 3-527-28318-8.KellyTurner (1999) Biotransformations -Practical Aspects in Biotechnology. 2 nd Edition, 25. Vol. 8a,M.Kelly (ed.). VCR-Wiley, ISBN 3-527-30104-6.DSebek and J. Rosazza (1995).Microbial Transfonnations in Kirk-Othmer Encyclopedia of ChemicalO.Technology. 4 th Edition 16, 611. Interscience-Wiley. ISBN 0-471-52685-1Prenosil, OÈ . Kut, I. Dunn, E. Reinzle (1989) Immobilized Biocatalysts in Ullmann's EncyclopediaJ.ofIndustrial Chemistry. 5 th edition A14. 1. Wiley-VCR, ISBN 3-52720114-9Duport, R. Spagnoli, E. Degryse, D. Pompon (1998) «Self-sufficient biosynthesis of pregnenoloneC.and progesterone in engineered yeast.» Nature Biotechnology: vol.16, p. 186.ller (1994). Steroids in Ullmann's Encyclopedia Chemistry 5 th edition A25, 309. Wiley-VCH,R.MuÈ3-527-20125-4.ISBNMiot and J. Boulay (2001). «Protein technologies and commercial enzymes.» Current Opinion inS.vol.12, p. 329.Biotechnology:Curtis (2000).Carbon-Carbon Bond FonnationUsing Enzymes in Biotechnology 2 nd Edition, 5.A.8b, D. Kelly (ed.). VCRWiley, ISBN 3-527-28324-2.VoL(2000) Enzymes in Carbohydrate Chemistry: Formation of Glycosidic Linkages inFlintsch,G.Watt2 nd Edition. 243. VoL 8b, D Kelly (ed.). VCR-Wiley, ISBN 3-527-28324-2.BiotechnologyKelly, J. Mahdi (2000) Lyases in Biotechnology 2 nd Edition, 41. Vol. 8b, D. Kelly (ed.). VCR-D.ISBN 3-527-28324-2.Wiley,527-28324-2.Shimizu,J.Ogawa, M. Kataoka, M. Kobayashi (1997), «Screening of novel microbial enzymesS.the production of biologically and chemically useful compounds.» Advances in Biochem-forical Engineering/Biotechnology: vol. 58, p. 45.J:(JAyGO*hQ+ATzy?*(+AGSteroid biotransformationJ9}*R!}GEnzymes406

S. Jackman, J. Stratford (2000).Halocompounds in Biotechnology 2 nd Edition, 173.G.Robinson,8b, D Kelly (ed.). VCR-Wiley, ISBN 3-527-28324-2.Vol.M. Woodley (2000). Advances in epzyme technology-UK contributions.» Advances in BiochemicalJ.Engineering/Biotechnology: vol.70, p. 93.Holland (1999).Hydroxylation and Dihy- Pharmadroxylation in Biotechnology 2 nd Edition, 475.H.Sa, D Kelly (ed.). VCH Wiley, ISBN 3-527-30104-6.Vol.Hoople (1999).Cleavage and Formation ofAmide Bonds in Biotechnology 2 nd Edition, 243.D.Sa, D Kelly(ed.). VCH-Wiley, ISBN 3-527-30104-6 Wiley, ISBN 3-527-30104-6.Vol.Foster, S. Frackman and J. Jolly (1995) Production of Enzymes as Fine Chemicals in BiotechnologyK.2 nd Edition, 73. Vol. 9, G Reed, T Nagodawithana (ed.). VCH-Wiley, ISBN 3-527-Scopes (1994) Protein Purification PrindpIes and Practice, 3 rd Edition ed., Springer-Verlag,R.0-387-94072-3.ISBNPerham, M. Grassl, G. Michal, B. Rexer, A. Scheltinga, C. GoÈ lker, S. Fukui, A. Tanaka, H.R.W. Goldstein, H. Hagen, S. Pedersen, B. Poldermans, E. Reimerdes, W. Leuchtenber-Uhlig,U. PloÈ cker, H. Waldmann, G. Whitesides, G-B Kresse, K. Wulff, G. Henninger, L.ger,,WGuÈ nzler, C. Kessler, K. Aunstrup (1987) Enzymes in Ullmann's Encyclopedia ofFlohteÂJaeger and T. Eggert (2002), «Lipases for biotechnology.» Current Opinion in Biotechnology:K.13, p. 390.vol.Fetzner (2002) «Oxygenases without requirement for cofactors or metalions.» Applied MicrobiologyS.and Biotechnology: vol. 60, p. 243.Panke, M. G. Wubbolts (2002), «Enzymetechnology and bioprocess engineering.» Current OpinionS.in Biotechnology: vol. 113, p.111.Schmid, J. S. Dordick, B. Hauer [et al.] (2001).»Industrial biocatalysis todayand tomorrow.»A.vol.409, p. 258.Nature:McGregor-Jones (2000). Synthetic Applications of Enzyme-Catalyzed Reactions in Biotechnology.J.2 nd Edition, 351. Vol. 8b, D. Kelly (ed.). VCH-Wiley, ISBN 3-52728324-2.T.Bornscheuer and R. J. Kazlauskas (1999). Hydroases in Organic Synthesis, Wiley-VCH,U.3-527-30104-6.ISBNBunch (1999) Nitriles in Biotechnology. 2 nd Edition, 277. Vol. 8a, D. Kelly (ed.). VCH-Wiley,A.3-527-30104-6.ISBNKazlauskas, U. Bornscheuer (1999) Biotransformations with Lipases in Biotechnology. 2 nd Edition,R.37. Vol. Sao, D.Kelly (ed.). VCH-Wiley, ISBN 3-527-30104-6Witiak and A. Hopper (1996) Chiral Pharmaceuticals in Kirk-Otthmer Encyclopedia of ChemicalD.Technology 4 th Edition 511. Interscience-Wiley, ISBN 0-471-52687-8Zaks (1994) Enzymes in Organic Synthesisin Kirk-Othmer Encyclopedia of Chemical TechnologyA.4 th Edition 9, 672 Interscience-Wiley, ISBN 0-471-52677-028319-6Industrial Chemistry 5 th edition A9, 341.Wiley-VCH, ISBN 3-527-20109-2,}*R!}GR+qJAyGEnzyme catalysis407

Usmani (1995). Medical Diagnostic ReagentsinKirk-Othmer Encyclopedia of Chemical TechnologyA.4 th Edition 168S. Interscience-Wiley, ISBN 0-47152685-1Kresse (1995).Analytical Use ofEnzymes inBiotechnology 2 nd Edition, 137. Vol. 9, G. Reed, T.G.(ed.). VCH-Wiley, ISBN 3-527-28319-6.NagodawithanaKopetzld, K. Lehnert, P. Buckel (1994). «Enzymes in diagnostics: achievementsand possibilitiesE.recombinant DNAS technology.» Clinical Chemistry: vol. 40, p. 688.ofUhlig(1998).Industrial Enzymes and their Applications. John Wiley and Sons, ISBN 0-471-H.19660-6.J9}*R!}G?+z+zJAyGAnalytical enzymes?+}*R!:GJGQ9=AL:GEnzymes testsP. Gherson, H. Lanza, M.Elavin, D. Vlastelica (1992). Automated Instrumenation, Clincal Chemistryin Kirk-Othmer Encyclopedia of Chemical Technology 4 th Edition 3, 751. Interscience-Wiley, ISBN 0S471-52671-1.J9p9\{vJ9}*R!}GEnzymes as additivesO. Kirk, T. V. Borchert, C. C. Fuglsang (2002). «Industrial enzyme applications.» Current Opinionin Biotechnology: vol. 13, p. 345.W. Aehle and O.Misset (1999) Enzymes for Industrial Applications in Biotechnology Second, CompletelyRevised Edition189. Vol. 5a, U. Ney, D. Schomburg(ed.), VCH Verlagsgesellschaft,ISBN 3-527-28315-3.H. Olsen (1995).Use of Enzymes in Food Processing in Biotechnology 2 nd Edition,663. vol. 9, G.Reed, T. Nagodawithana (ed.). VCH-Wiley, ISBN 3-527-28319-6.P. Nielsen, H. Malmos, T. Damhus, B. Diderichsen, H. Nielsen, M. Simonsen, H. Schiff, A. Oestergaard,H. Olsen, P. Eigtved, T. Nielsen, J. Xing (1994) Industrial Enzyme Applications inKirk-OthmerEncyclopedia of Chemical Technology 4 th Edition 9, 567. Interscience-Wiley,ISBN 0-471-52677-0.J9qd"CGJ9}*R!CDetergent enzymesR. Gupta, Q. K. Beg, P. Lorenz (2002) «Bacterial alkaline proteases: molecular approaches and industrialapplications.» Applied Microbiology and Biotechnology: vol. 59, 15 0-8247-9995-X.J. Hv Ee, O.Misset, E. J. Baas (1997).Enzymes in Detergency in Surfactant Science Series Vol. 69,M. J. Schick, F. M. Fowkes (ed.). Marcel Dekker, Inc., ISBN 0-8247 9995-X.J. Lynn (1993). Detergency in Kirk-Othmer Encyclopedia of Chemical Technology 4 th Edition 7,1072. Interscience-Wiley, ISBN 0-471-52675-4.G. Jakobi, A. LoÈ hr, M. Schwuger, D.Jung, W. Fischer, P. Gerike, K KuÈ nstler (1987) Detergents inUllmann's Encyclopedia ofIndustrial Chemistry 5 th edition A8,315. Wiley-VCH, ISBN 3-527-20108-4.408

R. Whistler andJ. Daniel (1997) Starch in Kirk Othmer Encyclopedia of Chemical Technology 4 thJ9}*R!C{+zJAyG,F9CG9W"zyEnzymes for starch hydrolysisC. Bertoldo and G. Antranikian (2002). «Starch hydrolyzing enzymes from thermophilic archaeaand bacteria.» Current Opinion in Chemical Biology: vol. 6, p. 151Edition 22, 699. Interscience-Wiley, ISBN 0-471-52691-6R. Daniel, R. Whistler, A. Voragen, W. Pilnik (1994). Starch and Other Polysaccharides inUllmann'sEncyclopedia of IndustrialChemistry. 5 th edition A25, 1. Wiley-VCH, ISBN 3-527-20125-4J9+zJCGhJ9}*R!}GEnzymes and sweetenersM. M. Silveira and R. Jonas (2002). «The biotechnological production of sorbitol.» Applied Microbiologyand Biotechnology: vol. 59, p. 400.R. Hebeda (1997).Syrups in Kirk-Othmer Encyclopedia of Chemical Technology 4 th Edition 23,582. Interscience- Wiley, ISBN 0-471-52692-4.T. Lee (1997).Sweeteners in Kirk-Othmer Encyclopedia of Chemical Technology4 th Edition 23,556. Interscience-Wiley, ISBN 0-471-52692-4R. Hebeda (1995) Carbohydrate-Based Sweeteners in Biotechnology 2 nd Edition, 737. Vol. 9, G.Reed, T. Nagodawithana(ed.). VCH-Wiley, ISBN 3-527-28319-6G-W.R. Lipinski (1995) Sweeteners inUllmann's Encyclopedia of Industrial Chemistry 5 th editionA26, 23. Wiley-VCH, ISBN 3-527-20126-2.H. Schiweck, M. Clarke (1994) Sugar in Ullmann's Encyclopedia of IndustrialChemistry 5 th editionA25, 345. Wiley - VCH, ISBN 3-527-20125-4.F. Schenck (1989) Glucose-Containing Syrupsin Ullmann's Encyclopedia of Industrial Chemistry5 th edition A12, 457 Wiley-VCH, ISBN 3-527-20112-2.JGO*OfhQwTyG R(y(z+TyGJ9}*R!C{+zJAyEnzymes for the hydrolysis of cellulose and polyosesN. Thompson (1995) Hemicellulose in Kirk-Othmer Encyclopedia of ChemicalTechnology 4 th Edition13, 54. Inter-science-Wiley, ISBN 0-471-52682-7.H. KraÈ ssig, J. Schurz,R. Steadman, K. Schliefer, W. Albrecht (1986) Cellulose in Ullmann's Encyclopediaof Industrial Chemistry 5 th edition A5, 375. WileyVCH, ISBN 3-527-20105-X.bQ(yGJ9}*R!C?"+Gf?@9g|Enzymes in pulp and paper processingL.Viikari, M. Tenkanen, A SuumaÈ kki (2001). Biotechnology in the Pulp and PaperIndustry in Biotechnology2 nd Edition,523. Vol. 10, H. Rehm (ed.). VCH-Wiley ISBN3-527-28320-XA. Gutierrez, J. C. del Rio, M. J.Martinez and A.T. Martinez (2001), «The biotechnologicalcontrolof pitch in paper pulp manufacturing.» Trends in Biotechnology: vol. 19, p. 340.M. Schulein (2000), «Protein engineering of cellulases.» Biochimica et Biophysica Acta, vol. 1543,p. 239.409

Breen, F. L. Singleton (1999). «Fungi in lignocellulose breakdown and biopulping.» CurrentA.in Biotechnology, vol. 10, p. 252.OpinionF. Dean, P. R. LaFayette, K. E. Eriksson, S. A. Merkle (1997), «Forest tree biotechnology.» AdvancesJ.in Biochemical Engineering/Biotechnology, vol. 57, p. 1.M. Tenkanen, J. Buchert, L. Viikari (1997), «Hemicellulases in the bleaching of chemicalA.Suumakki,pulps.» Advances in Biochemical Engineering/Biotechnology, vol. 57, p. 261.Genco (1996).Pulp in Kirk-Othmer Encyclopedia of Chemical Technology 4 th Edition 20, 493.J.ISBN 0-471-52689-4.Interscience.Wiley,Lyne (1996).Paper in Kirk-Othmer Encyclopedia of Chemical Technology 4 th Edition 18, 1. Interscience-Wiley,M.ISBN 0-471-52687-8.R. Kashyap, P. K. Vohra, S. Chopra, R.Tewari (2001). «Applications of pectinases in the commercialD.sector: a review.» Bioresource Technology: vol. 77 p. 215.Baird (1994) Gums in Kirk-Othmer Encyclopedia of Chemical Technology 4 th Edition 12, 842.J.ISBN 0-471-52681-9.Intersdence-Wiley,C. Hall (1995) Milk and Milk Products in Kirk-Othmer Encyclopedia of Chemical Technology 4 thA. Law and F. Mulholland (1991). «The influence of biotechnological developments on cheeseB.Biotechnology and Genetic Engineering Reviews, vol. 9, p. 369.manufacture.»Stein, K. Imhof (1990). Milk and Dairy Products in Ullmann's Encyclopedia of Industlial ChemistryJ.5 th edition A16, 589. Wiley-VCH, ISBN 3-527-20116-5.Belderok (2000) «Developments in bread-making processes Plant Foods.» Human Nutrition:B.55, p. 1.vol.Spicher and J. Brummer (1995) Baked Goods in Biotechnology 2 nd Edition, 241. Vol. 9, G.G.T. Nagodawithana (ed.). VCH-Wiley, ISBN 3-527-28319-6.Reed,Spicher and Y. Pomeranz (1985) Bread and Other Baked Products in Ullmann's EncyclopediaG.Industrial Chemistry 5 th edition A4, 331. Wiley-VCR, ISBN 3527-20104-1.ofHamlyn (1995), «The Impact of Biotechnology on the Textile Industry.» Textiles Magazine,P.3, p. 6. vol.Heideman (1990) Leather in Ullmann's Encyclopedia of Industrial Chemistry 5 th edition A15,E.Wiley-VCH, ISBN 3-527-20115-7.259.R9"+Aw=yGJ9}*R!CPectinases>+zAGJ9GA"|hJ9}*R!}GEnzymes and milk productsEdition 16, 700. Interscience-Wiley, ISBN 0-471-52685-1.J9}*R!CR=BG Q+];he(JzyG?f9"YEnzymes in baking and meet processingO(z@G?GT!}Gh?@9g|J9}*R!CEnzymes in leather and textile treatment410

Beilen and Z.Li (2002), «Enzyme technology: an overview.» Current Opinion in Biotechnology,J.13, p. 338.vol.T. Farinas, T. Bulter, F. H. Arnold (2001), «Directed enzyme evolution.» Current Opinion inE.12, p. 545.Biotechnology,vol.A.Powell, S. W.Ramer, S. B. Del Cardayre [et al.] (2001). «Directed Evolution and Biocatalysis.»K.Angewandte Chemie International Edition, vol. 40,p.3948.T. Reetz (2001). «Combinatorial and Evolution-Based Methods in the Creation of EnantioselectiveM.Catalysts.» Angewandte Chemie International Edition, vol. 40, p. 284.H. Arnold and J. C Moore (1997), «Optimizing industrial enzymes by directed evolution.» AdvancesF.in Biochemical Engineering/Biotechnology, vol. 58, p. 1.Caron (1995) Commerdal Production of Baker's Yeast and Wine Yeast in Biotechnology 2 nd Edition.C.321. Vol. 9. G. Reed and T. Nagodawithana (ed.). VCH-Wiley, ISBN 3-527-28319-6.Ratledge (1997) Microbial Lipids in Biotechnology 2 nd Edition. 133. Vol. 7, Hv. DoÈ hren and H.C.(ed.). VCH-Wiley, ISBN 3-527-28310-2.KleinkaufScrimshaw and E. Murray (1995) Nutritional Value and Safety of «Single Cell Protein» in BiotechnologyN.2 nd Edition, 221. Vol. 9, G. Reed, T. Nagodawithana (ed.). VCHWiley. ISBN 3-Litchfield (1994). Nonconventional Foods in Kirk-Othmer Encyclopedia of Chemical TechnologyJ. th Edition 11, 871. Interscience-Wiley, ISBN 0-471-52680-04Babel, H-D PoÈ hland and K. Soyez (1993) Single Cell Proteins in Ullmann's Encyclopedia of IndustrialW.Chemistry 5 th edition A24. 165. Wiley-VCH, ISBN 3-527. 20124-6Kjelleberg (2002). «Environmental biotechnology.»Current Opinion in Biotechnology, vol. 13,S.199.Riedel, G. Kunze and A. Konig (2002). «Microbial sensors on a respiratory basis forwastewaterK.Advances in Biochemical Engineering/Biotechnology, vol. 75, p. 81.monitoring.»Nisipeanu (1999) Laws, Statutory Orders and Directives on Waste and Wastewater Treatment inP.2 nd Edition, 141. Vol. 11a, J. Winter (ed.). VCH-Wiley, ISBN 3-527-28321-8.BiotechnologyGallert and J. Winter (2000).Perspectives of Waste, Wastewater, Off-Gas, and Drinking WaterC.in Biotechnology 2 nd Edition, 479. Vol. 11c, J. Winter (ed.). VCR-WHey, ISBNManagementJGAGQFEd(ZAG^fJ9}*R!C?+"t@IO*OFProcedure for obtaining novel technical enzymes?+qzgyG QF9}BGhR9=BG IQ+?Baker's yeast and fooder yeastsIOQq"CG#+@hQ

Fritsche and M.Hofrichter (2000).Aerobic Degradation by Microorganisms in BiotechnologyW. nd Edition, 145. Vol. 11 b, J. Klein (ed.). VCH-Wiley, ISBN 3-52728323-4.2(1999).Historical Development of Wastewater Treatment Processes in BiotechnologyL.Hartmann nd Edition, 5. Vol. 11a, J Winter (ed.). VCH-Wiley, ISBN 3-52728321-8.2Gallert and J. Winter (1999) Bacterial Metabolism in Wastewater Treatment in BiotechnologyC. nd Edition, 17. Vol. 11 a, J. Winter(ed.). VCH-Wiley, ISBN 3-527-2832182(1999) Activated Sludge Process in Biotechnology 2 nd Edition, 253. Vol. 11 a, J. WinterR.KayserVCR-Wiley, ISBN 3527-28321-8.(ed.).Baumann and B Dorias (1999) Trickling Filter Systems in Biotechnology 2 nd Edition, 335.P.11 a, J. Winter (ed.). VCR-Wiley, ISBN 3-527-28321-8.Vol.Koppe, A. Stozek, V. Neitzel (1999). Municipal Wastewater and Sewage Sludge in BiotechnologyP.2 nd Edition, 161. Vol. 11 a, J. Winter (ed.). VCR-Wiley, ISBN 3527-28321-8.(1999). Agricultural Waste and Wastewater Sources and Management in Biotechnology.P.Weiland nd Edition, 217. Vol. 11 a, J. Winter (ed.). VCR-Wiley, ISBN 3527-28321-8.2Kroiss and K. Svardal(1999). CSTR-Reactors and Contact Processes in Industrial Waste waterH.in Biotechnology 2 nd Edition, 479. Vol. 11a, J. Winter (ed.). VCH-Wiley, ISBN 3-TreatmentJordening, K Buchholz (1999). Fixed Film Stationary Bed and Fluidized Bed Reactors in BiotechnologyH.2 nd Edition, 493. Vol. 11 a, J. Winter (ed.). VCH-Wiley, ISBN 3-527-28321-8Andrews (1993) Aerobic Waste Water Process Models in Biotechnology Second, CompletelyG.Edition, 407. Vol. 4, K. SchuÈ gerl (ed.). VCR Verlagsgesellschaft, ISBN ISBN 3-527-RevisedGallelt and J. Winter (2002). «Solid and liquid residues as raw materials for biotechnology.»C.vol. 89, p. 483.Naturwissenschaften,Sekiguchi, Y.Kamagata and H. Harada (2001), «Recent advances in methane fermentationY.Current Opinion in Biotechnology, vol. 12, p. 277.technology.»B. Schink(2000).Principles of Anaerobic Degradation of-Organic Compounds in Biotechnology 2 nd(1999).Anaerobic Metabolism and its Regulation in Biotechnology 2 nd Edition, 455.M.McInerney11a, J. Winter (ed.). VCR-Wiley, ISBN 3-527-28321-8.Vol.Phythian (1999) Esterases in Biotechnology 2 nd Edition, 193. Vol. 8a, D. Kelly (ed.). VCR-Wiley,S.ISBN 3-527-30104-6.Rosenwinkel, U.Austermaml-Haun and R. Meyer (1999) Industrial Wastewater Sources andK.Strategies in Biotechnology 2 nd Edition, 191. Vol. 11 a, J. Winter (ed.). VCR-Wi-Treatmentley, ISBN 3-52728321-8.527-28321-8.Schramm and R. Amann (1999).Nucleic Acid-Based Techniques for Analyzing the Diversity,A.and Dynamics of Microbial Communities in Wastewater Treatment in Biotechnol-Structure,ogy 2 nd Edition, 85. Vol. 11 a, J. Winter (ed.). VCH-Wiley, ISBN 3527-28321-8.28314-5.?+FG($;yG?@9gCG)Iz}AG(J;]qyG?

kl (1999). Modeling of Biogas Reactors in Biotechnology 2 nd Edition, 527. Vol. 11 a, J.WinterH.MaÈ(ed.). VCH-Wiley, ISBN 3527-28321-8SchuÈ rbuÈ scher and C. Wandrey (1993) Anaerobic Waste Water Process Models in BiotechnologyD.Completely Revised Edition, 441. Vol. 4, K SchuÈ gerl (ed.). VCR Verlagsgesellschaft,Second,Engesser and T. Plaggemeier (2000). Microbiological Aspects ofBiological Waste Gas PurificationK.in Biotechnology 2 nd Edition, 275. Vol. 11c, J. Winter (ed.). VCR-Wiley, ISBN 3,527-Fischer (2000). Biofilters in Biotechnology 2 nd Edition, 321. Vol. 11c, J. Winter (ed.). VCR-Wiley,K.ISBN 3-527-28336-6.Chitwood and J. Devinny (2000).Commerdal Applications ofBiological Waste Gas PurificationD.Biotechnology 2 nd Edition, 357. Vol. 11c, J. Winter (ed.). VCR-Wiley, ISBN 3-527-28336-inPlaggemeier and OÈ .LaÈ mmerzahl (2000). Treatment of Waste Gas Pollutants in Trickling FiltersT.Biotechnology 2 nd Edition, 333. Vol. 11c, J. Winter (ed.). VCR-Wiley, ISBN 3-527-28336-inReiser (2000).Waste Gas Treatment: Membrane Processes and Alternative Techniques in BiotechnologyM.2 nd Edition, 345. Vol. 11c, J. Winter (ed.). VCR-Wiley, ISBN 3527-28336-6.Schippert and R. Chmiel (2000) Bioscrubbers in Biotechnology 2 nd Edition, 305. Vol. 11c, J.E.(ed.). VCR-Wiley, ISBN 3527-28336-6.WinterWaweru, V. Rerrygers, H. V. Langenhove and W. Verstraete (2000). Process Engineering ofBiologicalM.Waste Gas Purification in Biotechnology 2 nd Edition, 259. Vol. 11c, J. Winter (ed.).(2000).Contaminated Soil Areas, Different Countries and Contaminants, Monitoring ofW.Ullriciin Biotechnology 2 nd Edition, 5. Vol. 11b, J. Klein (ed.). VCR-Wiley, ISBN 3-ContaminantsKlein (2000).Possibilities, Limits, and Future Developments of Soi1 Bioremediation in BiotechnologyJ.2 nd Edition, 465. Vol. 11b, J. Klein (ed.). VCR-Wiley, ISBN 3-52728323-4.Koning, K. Rupe and R. Stegmann (2000). Thermal Processes, Scrubbing/Extraction,BioremediationM.and Disposal in Biotechnology 2 nd Edition, 305. Vol. 11b, J. Klein (ed.). VCR-Wiley,and R. DoÈ rr (2000). In situ Remediation in Biotechnology 2 nd Edition, 349. Vol.11b, J.T.Held(ed.). VCR-Wiley, ISBN 3527-28323-4.KleinISBN 3527-28314-5.Biological treatment of exhaust aireO9gyG?+F(y(+=yG?@9gCGAG(%y28336-6.6.6.VCR-Wiley, ISBN 3-52728336-6.Biological soil treatment?+F(y(+=yG?@9gCG?

Unterman, M. DeFlaun and R. J. Steffan (2000). Acvanced in situ Bioremediation -A HierarchyR.Technology Choices in Biotechnology 2 nd Edition, 399. Vol. 11b, J Klein (ed.). VCR-Wiley,ofC. Wischnak and R. MuÈ ller (2000).Degradation of Chlorinated Compounds in Biotechnology 2 ndleaching, biofilms,MicrobialbiocorrosionandBuckel (1996). «Recombinant proteins for therapy.» Trends in Pharmacological Sciences, vol. 17,P.450. p.ISBN 3-527-28323-4Edition, 241. vol. 11b, J. Klein (ed.). VCR-Wiley, ISBN 3-527-28323-4.V. Schulz-Berendt (2000).Bioremediation with Heap Technique in Biotechnology 2 nd Edition, 319.Vol. 11b, J. Klein (ed.). VCR-Wiley, ISBN 3-527-28323-4.K. Blotevogel and T. Gorontzy (2000).Microbial Degradation of Compounds with Nitro Functionsin Biotechnology 2 nd Edition,273. Vol. 11b, J. Klein (ed.). VCR-Wiley, ISBN 3-527-28323-4.M. KaÈ stner (2000).»Humification» Process or Formation ofRefractory Soil Organic Matter in Biotechnology2 nd Edition, 89. Vol. 11b, J Klein (ed.). VCR-Wiley, ISBN 3527-28323-4.F-M Menn, J. Easter and G. Sayleri (2000). Genetically Engineered Microorganisms and Bioremediationin Biotechnology 2 nd Edition, 5. Vol. 11b, J. Klein (ed.). VCR-Wiley, ISBN 3-527-28323-4.W. Chen, F. Bruhlmann, R. D. Richins and A Mulchandani (1999).»Engineering of improved microbesand enzymes for bioremediation.» Current Opinion in Biotechnology: vol. 10, p. 137.R. Prince (1998) Bioremediation in Kirk-Othmer Encyclopedia of Chemical Technology 4 th EditionSupplement, 48. Interscience-Wiley, ISBN 0-471-52696-7.M. Dua, A. Singh, N. Sethunathan, A. K. Johri (2002), «Biotechnology and bioremediation: successesand limitations.»Applied Microbiology and Biotechnology, vol. 59, p. 143.M9W@Q:G?+Wi}Gh,?*(+AG{vzAyGh j(+AG )BAG(,jhQw+CGR. C. Hemming (2002). «Biofouling in water systems -cases, causes and counter Measures.» AppliedMicrobiology and Biotechnology, vol. 59, p. 629.H. Brandl (2001).Microbial Leaching ofMetals in Biotechnology 2 nd Edition, 191. Vol. 10, H.Rehm (ed.). VCH-Wiley, ISBN 3527-28320-X.G. Gadd (2001).Accumulation and Transformation of Metals by Microorganisms in Biotechnology.2 nd Edition, 225. vol. 10, H. Rehm (ed.). VCH-Wiley, ISBN 3-52728320-X.W. Sand (2001). Microbial Corrosion and its Inhibition in Biotechnology 2 nd Edition, 265. Vol. 10,H. Rehm (ed.). VCH-Wiley, ISBN 3-527-28320-X.H. Ehrlich (1997). «Microbes and metals.» Applied Microbiology and Biotechnology, vol. 48, p.687.?+=ayG?*(+AG?!9tAyGMedical biotechnologyP. Buckel (1998). «Toward a new naturalmedicine.» Naturwissenschaften: vol. 8, p. 155.414

Shoelson (1995).Insulin and Other Antidiabetic Agents in Kirk-Othmer ncyclopedia of ChemicalS.4 th Edition 14, 662.Interscience-Wiley, ISBN 0-471-52683-5TechnologyF. Bristow (1993).»Recombinant-DNA-derived insulin analogues as potentially useful therapeuticA.agents.»Trends in Biotechnology, vol. 11, p. 301.Schmidt (1985) Antidiabetic Drugs in Ullmann's Encyclopedia of Industrial Chemistry 5 th editionF.A3, 1. Wiley-VCH, ISBN 3-527-20103-3.H. Frank and R. E. Chance (1983). «Two routes for producing human Insulin utilizing recombmantB.DNA technology.» Muench.med. Wschr., vol. 125, p. 14.Sandow, E. Scheiffele, M. Haring, G. Neef, K.Prezewowsky and U. Stache (1989). Hormones inJ.Encyclopedia of Industrial Chemistry 5 th edition A13, 89. Wiley-VCH, ISBN 3-Ullmann'sM. Chang (1999), «Future prospects for artificial blood.» Trends in Biotechnology, vol. 17, p.T.61.Bell (1992).Blood, Coagulants and Anticoagulants in Kirk-Othmer Encyclopedia of ChemicalW.4 th Edition 4,333. Interscience-Wiley, ISBN 0-47152672-X.TechnologyYamashita (1985) Blood in Ullimann's Encyclopedia of Industrial Chemistry 5 th edition A4, 201.S.ISBN 3-527-20104-1.Wiley-VCH,Jiang, T. Monroe, R. McRogers, P. J. Larson (2002). «Manufacturing challenges in the commercialR.production of recombinant coagulation factor VIII.» Haemophilia, vol. 8 Suppl.1.Soukharev, D. Hammond, N. M. Ananyeva, J. A. Anderson, C. A. Hauser, S. Pipe, E. L. SaenkoS.(2002). «Expression of factor VIII in recombinant and transgenic systems Blood Cells.»G. D. Tuddenheim (1997). Haemophilia: Molecular biology at the centre of human disease in MolecularE.Biology in Medicine, 1. TM Cox, J. Sinclair (ed.). Blackwell Science Ltd., ISBN 0-G#+y(T!Insulinf(|Q$J9!(|Q%yG?+t

H. Bendixen and L. Ocava (2002). «Evaluation and management of acute ischemic stroke.» CurrentB.Cardiology Reports, vol. 4, p. 149.Bode and H. Renatur (1997) «Tissue-type plasminogen activator: variants and crystal/solutionW.demarcate structural determinants of function.» Current Opinion in StructuralstructuresJayaram, G. Ahluwalia and D. Cooney (1994).Therapeutic Enzyme Applications in Kirk-OthnnerH.Encyclopedia of Chemical Technology 4 th Edition 9, 621. Interscience-Wiley, ISBN 0-Muscate, C. Levinson and G. Kenyon (1994). Enzyme Inhibitors in Kirk-Othmer EncyclopediaA.Chemical Technology 4 th Edition 9, 646 Interscience-Wiley, ISBN 0-471-52677-0.ofCorthesy (2002). «Recombinant immunoglobulin A: powerful tools for fundamental and appliedB.research.»Trends in Biotechnology, vol. 20, p. 65.Lanu (2001). «Adult stem cells: an alternative to embryonic stem cells?.» Trends in Biotechnology,M.vol. 19, p. 487.and A. Kind (2000). «Therapeutic cloning: concepts and practicalities.» Trends in Biotechnology,AColmanvol. 18, p. 192.A. Thomson and J. S. Odorico (2000). «Human embryonic stem cell and embryonic genu cellJ.Trends in Biotechnology, vol. 18 p. 53.lines.»V. Risbud and M Sittinger (2002). «Tissue engineering: advances in in vitro cartilage generation.»M.Trends in Biotechnology, vol. 20, p. 351.G. Griffith and G. Naughton (2002). «Tissue engineering-current challenges and expanding opportunities.»L.Science, vol. 295, p.1009.JGO9]|QDMAyG)bzGAyG({|G(gyGh?y9AGIQDMzyAnticoagulants and thrombolytic agentsF. Markwardt (2002), «Hirudin as alternative anticoagulant-a historical review.» Semin ThrombHemost, vol. 28, p. 405.Biology, vol.6, p. 865.471-52677-0.J9}*R!:GJ9a=D|Enzyme inhibitors,f9"CGR9%@GTheImmunesystemC. Janeway and P Travers (1997). Immunobiology. 3 rd Edition. Current Biology, 0443-05964-0.?+fP@G9*;BGStem cellsG. Daley (2002), «Prospects for stem cell therapeutics: myths and medicines.» Current Opinion inGenetics and Development, vol. 12, p. 607.?GT!}G?SO"$Tissue engineeringP. Bianco and P. G. Robey (2001). «Stem cells in tissue engineering.» Nature, vol.414, p. 118.416

Wetzel (1999) Medical Applications of Recombinant, Proteins in Humans and Animals in BiotechnologyG.Second, Completely Revised Edition, 125. Vol. 5a, U. Ney, D. Schomburg (ed.).Wong and J. Xing (1995) Immunotherapeutic Agents in Kirk-Othmer Encyclopedia of ChemicalS.4 th Edition 14,64. Interscience-Wiley. ISBN 0-471: 52683-5TechnologyNagabhushan and P Trotta (1989). Inteiferons in Ullmann's Encyclopedia of Industrial ChemistlyT.5 th edition A14, 365. Wiley-VCH, ISBN 3-527-20114-9.Friehs andK. F Reardon (1993). «Parameters influencing the productivity of recombinant E.K.cultivations.» Advances in Biochemical Engineering/Biotechnology, vol. 48, p. 53.coliS. Lubiniecki and J. H. Lupker (1994). «Purified protein products of rDNA technology expressedA.in animal cell culture.» Biologicals, vol. 22, p. 161.Schiffmann and R. O. Brady (2002). «New prospects for the treatment of lysosomal storage diseases.»R.Drugs, vol. 62, p. 733.E. Kearney and C. E. Wallis (2000). «Deoxyribonuclease for cystic fibrosis Cochrane Database-C.Rev. CD001127.SystHsieh and M. Ritchey (1997). Vaccine Technology in Kirk-Othmer Encyclopedia of ChemicalC.4 th Edition 24. 727. Interscience-Wiley, ISBN 0-47152693-2.TechnologyCryz, M. Granstrom, B. Gottstein, L Perrin, A. Cross, J. Larrick (1989). Immunothetpay andS.in Ulhnann's Encyclopedia of Industrial Chemistry 5 th edition A14, 49. WileyVaccinesX. Berthet, T. Coche and C. Vinals (2001), «Applied genome research in the field of human vaccines.»F.Journal of Biotechnology, vol. 85, p. 213.Olive, I. Toth and D. Jackson (2001). «Technological advances in antigen delivery and syntheticC.vaccine developmental strategies.» Mini-Reviews in Medicinal Chemistry, vol. 1, p. 429peptideM. Walmsley and C. J.Arntzen (2000), «Plants for delivery of edible vaccines.» Current OpinionA.Biotechnology, vol. 11, p. 126.inRajewsky (1996). «Clonal selection and learning in the antibody system.» Nature, vol. 381,K.751. p.GInterferonsJ9!hQ+pQA!VCH Verlagsgesellschaft, ISBN 3-527-28315-3.GInterleukinsJ9"+v(yQA!iQLC?+F;fJ9"+@hQ

Galfre, D. Secher and P. Crawley (1990).Monoclonal Antibodies in Ullmann's Encyclopedia ofG.Chemistry 5 th edition A16, 699. Wiley-VCH, ISBN 3527-20116-5IndustrialD.Wittrup (2001). «Protein engineering by cell-surface display.» Current Opinion in Biotechnology,K.vol. 12, p.395.E. Chadd and S. M. Chamow (2001), «Therapeutic antibody expression technology.» CurrentH.in Biotechnology, vol. 12, p. 188.OpinionBlackburn and A. Garcon (2000) Catalytic Antibodies in Biotechnology 2 nd Edition, 491.G.8b, D. Kelly (ed.). VCH Wiley, ISBN 3-527-28324-2.Vol.Adair (1999) Antibody Engineeringand Expression in Biotechnology Second, Completely RevisedJ.219. Vol. 5a, U. Ney and D. Schomburg (ed.). VCH Verlagsgesellschaft, ISBN 3-Edition,Racher, J. Tong andJ Bonnerjea (1999). Manufacture of Therapeutic Antibodies in BiotechnologyA.Second. Completely Revised Edition, 247. Vol. 5a, U. Ney, D. Schomburg (ed.). VCHDriggers and P. G. Schultz (1996). «Catalytic Antibodies.» Advances in Protein Chemistry,E.p. 261.vol.49,Miller and R. Niessner (1994) Enzyme and Immunoassays in Ullmann's Encyclopedia of IndustrialJ.Chemistry 5 th edition B5, 129. Wiley-VCH, ISBN 3-527-20135-1.W. Scheller, U. Wollenberger, A. Warsinke and F. Lisdat (2001), «Research and developmentF.biosensors.» Current Opinion in Biotechnology, vol. 12, p. 35.inL. Rich and D. G. Myszka (2000), «Advances in surface plasmon resonance biosensor analysis,»R.Current Opinion in Biotechnology, vol. 11, p. 54.Cammann, B. Ross, W. Hasse, C. Dumschat, A. Katerkamp, J.Reinbold, G Steinhage, B.K.R. Renneberg and N. Buschmann (1994) Chemical and Biochemical Sensors in Ull-Griindig,Encyclopedia of Industrial Chemistry 5 th edition B6, 121. Wiley-VCH, ISBN 3-mann'sS2720136-X.Mattiasson (1993). Biosensors in Biotechnology Second, Completely Revised Edition, 5. Vol. 4,B.SchuÈ gerl (ed.). VCH Verlagsgesellschaft, ISBN 3-52728314-SKPietro (1992) Biosensors in Kirk-Othmer Encyclopedia of Chemical Technology 4 th Edition 4,W.Interscience-Wiley, ISBN 0471-52672-X.208.C. A.Janeway, Jr. (1993). «How the immune system recognizes invaders.» Scientific American,vol.269, p. 72.e9TF}GMonoclonal antibodies?z+T"yGIO+IhIO9]CGRecombinant and catalytic antibodies?*R+qJAyGh?

Wolf, V. Zakhartchenko and G. Brem (1998), «Nuclear transfer in mammals: recent developmentsE.and future perspectives.» Journal of Biotechnology, vol. 65, p. 99.H. Yue, P. Beeckmann, H Bartenschlagel, G. Moser and H. Geldermann (1999), Rapid ad preciseG.genotyping ofporcine microsatellites.» Electrophoresis, vol. 20, p. 3358.Lucy and R. Collier (1994) Genetic Engineering, Animals in Kirk-Othmer Encyclopedia of Chemical.M.Technology 4 th Edition 12, 465 Interscience-Wiley, ISBN 0-471-52681-9.Geldermann (1990) Application of Genome Analysis in Animal Breeding in Genome Analysis inH.Animals Vol. H Geldermann. F Ellendorf (ed.). VCH Verlagsgesellschaft, ISBN 3-DomesticTrounson (2001). «Nuclear transfer in Imman medicine and animal breeding.» Reproduction,A.and Development, vol. 13, p. 31.FertilityMetzger and R. Feil (1999), «Engineeling the mouse genome by site-specific recombination.»D.Opinion in Biotechnology, vol. 10, p. 470.CurrentBrem (1993) Transgenic Animalin Biotechnology Second, Completely Revised Edition, 745.G.2. A. PuÈ hler (ed.). VCH Verlagsgesellschaft, ISBN 3527-28312-9,Vol.Brasile, B. M. Stubenitsky, G. Kootstra (2002). «Solving the organ shortage: potential strategiesL.the likelihood of success.» ASAIO Journal, vol. 248, p. 211.andW.Larrick and D. W. Thomas (2001). Producing proteins in transgenic plants and animals.»J.Opinion in Biotechnology,vol. 12, p. 411.CurrentS. Rudolph (1999), «Biopharmaceutical production in transgenic livestock.» Trends in Biotechnology,N.vol. 17, p. 367.Huang, C. Pray and S. Rozelle (2002). «Enhancing the crops to feed the poor.» Nature, vol. 418,J.678. p.?+*QjyG?fGQRGene farming and xenotransplantationA. Dove (2000), «Milking the genome for profit.» Nature Biotechnology, vol.18, p. 1045.J9="yG{+Yz@h?+

P. Tengerdy and G. Szakacs (1998). «Perspectives in agrobiotechnology.» Journal of Biotechnology,R.vol. 66, p. 91.W. Grout (1999). «Meristem-tip culture for propagation and virus elimination.» Methods inB.Biology, vol. 111, p. 115.MolecularM. Kieran, P. F. MacLoughlin and D. M. Malone (1997), «Plant cell suspension cultures: someP.considerations.» Journal of Biotechnology, vol. 59, p. 39.engineeringPetersen and A. W. Alfermann (1993) Plant Cell Cultures in Biotechnology Second, CompletelyM.Edition, 577. Vol. 1, H. Sahm (ed.). VCH Verlagsgesellschaft, ISBN 3-527-28337-4.RevisedTanaka (1987). «Large-scale cultivation of plant cells at high density: a review.» Process Biochemistry,A.p.106T. Verrips, M. M. Warmoeskerken and J. A. Post (2001), General introduction to the importanceC.of genomics in food biotechnology and nutrition.» Current Opinion in Biotechnology,Parkin, S. Robinson, A. Sharpe, K. Rozwadowski, D. Hegedus, D. Lydiate (2001). Agri-FoodI.Genomics in Biotechnology 2 nd Edition, 145. Vol. 5b, C. Sensen (ed.). VCHWiley, ISBNandYQGR|?+JaTyGJ9="yG?GT!CPlant tissue surface culture9*;LYQGR|?tzgCGJ9="yGPlant cell suspension cultureS. Jennewein and R. Croteau (2001). «Taxol: biosynthesis, molecular genetics, and biotechnologicalapplications.» Applied Microbiology and Biotechnology, vol. 57, p. 13.J. Berlin (1997).Secondary Products from Plant Cell Cultures in Biotechnology 2 nd Edition, 593.Vol. 7, Hv. Dohren, H. Kleinkauf (ed.). VCH-Wiley, ISBN 3-52728310-2.S. C Roberts and M. L. Shuler (1997). «Large-scale plant cell culture.» Current Opinion in Biotechnology,vol. 8, p. 154.uFGQayGIQ(JCGJ9@9="yGTransgenic plants: methods+CGQh :9 kA. Vanavichit, S. Tragoonrung and T. Toojinda (2001) Genomic Mapping and Positional Cloningwith Emphasis on Plant Science in Biotechnology 2 nd Edition, 165. Vol. 5b, C. Sensen (ed.).VCH-Wiley, ISBN 3527-28328-5.M. Hughes (1996).Plant Molecular Genetics.Longman. ISBN 0-582-24730-6.J. Edwards, G. Kishore and D. Stark (1994).Genetic Engineering,Plants in Kirk-Othmer Encyclopediaof Chemical Technology 4 th Edition 12, 491. Interscience-Wiley, ISBN 0-471-52681-9.G. Kahl and K Weising (1993). Genetic Engineering of Plant Cells in Biotechnology Second, CompletelyRevised Edition, 547. Vol. 2, A. PuÈ hler (ed.). VCH Verlagsgesellschaft, ISBN 3-527-28312-9?|h9tCGIQ(JCGJ9@9="yGTransgenic plants: resistance+CGQh :9 kvol. 12, p. 483.3-527-28328-5.420

+CGQh :9 kJ. Mol, E. Cornish, J. Mason and R. Koes (1999), «Novel coloured flowers.» Current Opinion inBiotechnology, vol. 10, p. 198.J9@9="yGTransgenic plants: productsJ9GA"CGIQ(JCGJ. W.Larrick and D. W. Thomas (2001), «Producing proteins in transgenic plants and animals.»Current Opinion in Biotechnology, vol. 12, p. 411.G. Giddings (2001), «Transgenic plants as protein factories.» Current Opinion in Biotechnology,vol. 12, p. 450.S. A. Merkle and J. F. Dean (2000), «Forest tree biotechnology.» Current Opinion in Biotechnology,vol. 11, p. 298.Y. Poirier (1999), «Production ofnew polymeric compounds in plants.» Current Opinion in Biotechnology,vol. 10, p. 181.L. Willmitzer (1993). Transgenic Plants in Biotechnology Second, Completely Revised Edition,627. Vol. 2, A. PuÈ hler (ed.). VCR Verlagsgesellschaft, ISBN 3527-28312-9.VirusesJ9ShQ+qyGP. Ahlquist (2002). «RNA-dependent RNA polymerases, viruses, and RNA silencing.» Science,vol. 296, p. 1270.E.Baranowski, C. M. Ruiz-Jarabo and E. Domingo (2001), «Evolution of cell recognition byviruses,» Science, vol. 292, p. 1102.J. L. Dangl and J. D.Jones (2001), «Plant pathogens and integrated defence responses to infection.»Nature, vol. 411, p. 826.A. J. McMichael, S. L. Rowland-Jones (2001). «Cellular immune responses to HIV.» Nature,vol.410, p. 980.R. M.Zinkernagel (1996), «Immunology taught by viruses.»Science, vol.271, p. 173.bacteriophagesJ9+C9gyGH. Sandmeier and J. Meyer (1993) Bacteriophages in Biotechnology Second, Completely RevisedEdition, 543. Vol. 1, H. Sahm (ed.). VCH Verlagsgeseschaft, ISBN 3-527-28337-4Microorganisms?*Q%GCGJ9"F9wyGC. Bertoldo, R. Grote and G. Antranikian (2001) Biocatalysis under Extereme Conditions in Biotechnology2 nd Edition, 61. Vol. 10, R. Rehm (ed.). VCH-Wiley, ISBN 3527-28320-X.Amann, B. M. Fuchs and S. Behrens (2001). «The identification of microorganisms by fluorescencein situ hybridization.» Current Opinion in Biotechnology, vol. 12, p. 231.H. J. Busse, E. B. Denner and W. Lubitz (1996), «Classification and identification ofbacteria: currentapproaches to an old problem: Overview of methods used in bacterial systematic.» Journalof Biotechnology, vol. 47, p. 3.421

Bahl and P. DuÈ rre (1993) Methylotrophs in Biotechnology Second, Completely Revised Edition,H.Vol. 1, H. Sahm (ed.).VCH Verlagsgesellschaft. ISBN 3-527 28337-4.285.nig (1993). Methanogens in Biotechnology Second, «Completely Revised Edition. 251. Vol. 1,RKoÈSahm (ed.). VCH Verlagsgesellschaft, ISBN 3-52728337-4.H.Dijkhuizen (1993).Methylotraphs in Biotechnology Second, Completely Revised Edition, 265.L.1, H. Sahm (ed.). VCR Verlagsgesellschaft, ISBN 3527-28337-4.Vol.G. Priest (1993) Bacillus in Biotechnology Second Completely Revised Edition, 367. Vol. 1, R.F.(ed.). VCR Verlagsgesellschaft, ISBN 3-527-28337-4SahmAuling (1993).Pseudomonads in Biotechnology Second, Completely Revised Edition, 401 Vol. 1,G.Sahm (ed.). VCR Verlagsgesellschaft, ISBN 3-527-283374.H.Piepersberg (1993).Streptomycetes and Corynebacteria in Biotechnology Second, CompletelyW.Edition, 433. Vol. 1, H. Sahm (ed.). VCH Verlagsgesellschaft, ISBN 3-527-28337-4.RevisedJ. Punt, N. van Biezen and A. Conesa [et al.] (2002). «Filamentous fungi as cellfactories for heterologousP.protein production.» Trends in Biotechnology, vol. 20, p. 200.R. Swartz (2001). «Advances in Escherichia coli production of therapeutic proteins.» CurrentJ.in Biotechnology, vol. 12, p. 195.OpinionBlattner, G. Plunkett, C. Bloch, N. Perna, V. Burland, M. Riley, J.Collado-Vides, J. Glasner,F.Rode, G. Mayhew, J. Gregor, N. Davis, H. Kirkpatrick, M. Goeden, D. Rose, B. MauC.Y. Shao (1997), «The Complete Genome Sequence ofEscherichia coli K-12.» Science,and277, p. 1453.vol.Meinhardt and K. Esser (1993) Filamentous Fungi in Biotechnology Second. Completely RevisedF.Edition, 515, Vol. 1, R. Sahm (ed.). VCR Verlagsgesellschaft, ISBN 3-527-28337-4P. Cereghino and J. M. Cregg (1999). Applications of yeast in biotechnology: protein productionG.and genetic analysis.» Current Opinion in Biotechnology, vol. 10, p. 422.Maloney (1998). Yeasts in Kirk-Othmer Encyclopedia of Chemical Technology 4 th Edition 25,D.Interscience-Wiley, ISBN 0-471-52694-0.761.J. Heinisch and C. P. Hollenberg (1993). Yeasts in Biotechnology Second, Completely RevisedJ.469. vol. 1, H. Sahm (ed.). VCH Verlagsgesellschaft, ISBN 3-52728337-4.Edition,K. Frobel and S. Metzger (2001) New Methods of Screening in Biotechnology in Biotechnology 2 nd9*QAw=yGbacteriaJ9!9tAyG?|9%yG9*QAw=yG?*(+AG,p^g

Crueger (1993). Mutagenesis in Biotechnology Second, Completely Revised Edition, 5. Vol. 2,A.PuÈ hler (ed.). VCH Vedagsgesellschaft, ISBN 3-527-283129.A.Engels, B. Sprunkel and E. Uhlmann (1993) DNA Synthesis in Biotechnology Second, CompletelyJ.Revised Edition, 317. Vol. 2, A. PuÈ hler (ed.). VCH Vedagsgesellschaft, ISBN 3-527-Recio (1990) Mutagenic Agents in Ullmann's Encyclopedia of Industrial Chemistry 5 th editionL.755. Wiley-VCH, ISBN 3-527-20116-5.A16,Stoppok and K. Buchholz (1996). Sugar-Based Raw Materials for Fermentation Applications inE.2 nd Edition, 5. Vol. 6, M. Roehr (ed.). VCH-Wiley, ISBN 3-527-28316-1.BiotechnologyD. Troostembergh (1996) Starch-Based Raw Materials for Fermentation Applications in BiotechnologyJ.2 nd Edition, 31. Vol. 6, M Roehr (ed.). VCH-Wiley, ISBN 3-52728316-1.Schneider, H. S. Teinmiiller (1996) Raw Material Strategies-Economical Problems in BiotechnologyF.2 nd Edition, 47. Vol. 6, M Roehr (ed.). VCH-Wiley, ISBN 3527-28316Sengha (1994). Fermentation in Kirk-Othmer Encyclopediaof Chemical Technology 4 th EditionS.361. Interscience-Wiley, ISBN 0-471-52678-910,Greasham (1993). Media for Microbial Fermentations in Biotechnology Second, Completely RevisedR.Edition, 127. vol. 3, G. Stephanopoulos (ed.). VCH Vedgsgesellschaft, ISBN 3-527-H Posten and C. L. Cooney (1993) Growth ofMicroorganisms in Biotechnology Second. CompletelyC.Revised Edition, 111. Vol. 1, H. Sahm (ed.). VCH Vedagsgesellschaft. ISBN 3-527-Yamane and S. Shimizu (1984) «Fed-Batch Techniques in Microbial Processes.» Advances inT.Engineering/Biotechnology, vol. 30, p. 147.BiochemicalH. Lee and V. Hatzimanikatis (2002). «Biochemical Engineering.» Current Opinion in Biotechnology,K.vol. 13, p. 85.Katzen and G. T. Tsao (2000). «A view of the history of biochemical engineering Advances inR.Engineering/Biotechnology, vol. 70, p. 77.BiochemicalJ9"F9wyG:?*Q%GCG_T;J:;TyGMicroorganisms: strain improvement28312-9.Growing microorganisms?*Q%GCGJ9"F9wyG ?+}"@28313-7.{+wW@J9+vQIGrowth kinetics and product formationHA"CGJ9+z}fh(}"yG28337-4Fed-batch and continuous fermentationQ+}MAyGh Q}ATCG IGPjCGQ+}MAyG?gpOy9

B. Tarmy (1996). Reactor Technology in Kirk-Othmer Encyclopedia of Chemical Technology 4 thK. Schugerl (2000), «Development of bioreaction engineering.» Advances in Biochemical Engineering/Biotechnology,vol. 70, p. 41.R. Kleijntjens and K. Luyben (2000) Bioreactors in Biotechnology 2 nd Edition,329.Vol.11b,J.Klein (ed.). VCH-Wiley, ISBN 3527-28323-4.G. Larsson, S. B. Jorgensen, M. N. Pons, B. Sonnleitner, A. Tijsterman and N Titchener-Hooker,(1997), «Biochemical engineering science.» Journal of Biotechnology, vol. 59, p. 3.Edition 20,1006. Interscience-Wiley, ISBN 0-471-52689-4.M. Reuss (1993). Oxygen Transfer and Mixing: Scale-Up Implications in Biotechnology Second.Completely Revised Edition, 185. Vol. 3, G Stephanopoulos (ed.) VCH Verlagsgesellschaft,ISBN 3-52728313-7.B. Buckland and M. Lilly (1993). Fermentation an Overview in Biotechnology. Second, CompletelyRevised Edition, 7. Vol. 3, G. Stephanopoulos (ed.). VCH Verlagsgesellschaft, ISBN 3-527-28313-7.J. Nielsen and J. Villadsen (1993) Bioreactors: Description and Modelling in Biotechnology Second,Completely Revised Edition,77. Vol. 3, G. Stephanopoulos (ed.). VCH Verlagsgesellschaft,ISBN 3-52728313-7.H. Voss (1992) Bioreactors in Ullmann's Encyclopedia of Industrial Chemistry 5 th edition B4, 381.Wiley-VCH, ISBN 3527-20134-3.Fermentation technology: scale-up:Q+}MAyGi(AT| G L9A! hpQ?!9t@B. Sonnleitner (2000), «Instrumentation of biotechnological processes.» Advances in BiochemicalEngineering/Biotechnology, vol.66, p.1.G. Seidel, C. Tollnick, M. Beyer, K. Schugerl (2000), «On-line and off-line monitoring of the productionof cephalosporin C by Acremonium chrysogenum.»Advances in Biochemical Engineering/Biotechnology,vol. 66, p.115.H. J. Henzler (2000), «Particle stress in bioreactors.» Advances in Biochemical Engineering/Biotechnology,vol. 67, p.35.D. A.Mitchell, M. Berovic and N. Krieger (2000), «Biochemical engineering aspects of solid statebioprocessing.» Advances in Biochemical Engineering/Biotechnology, vol. 68, p. 61.W. Beyeler, E. DaPra and K. Schneider (2000), «Automation of industrial bioprocesses.» Advancesin Biochemical Engineering/Biotechnology, vol. 70, p. 139.T. Chattaway, G. Montague and A.Morris (1993). Fermentation Monitoring and Control in BiotechnologySecond, Completely Revised Edition, 319, vol. 3, G Stephanopoulos (ed.). VCHVerlagsgesellschaft, ISBN 3-527-28313-7.L. Erockson, D. Fung, P. Tuitemwong (1993) Anaerobic Fermentations in Biotechnology Second,Completely Revised Edition, 7. vol. 3, G. Stephanopoulos (ed.). VCH Verlagsgesellschaft,ISBN 3-52728313-7.424

Bardouille (2001). Maintenance of Cell Cultures -with Special Emphasis on Eukaryotic Cells inC.2 nd Edition, 27. Vol. 10, H. Rehm(ed.). VCH-Wiley, ISBN 3-527-28320-X.BiotechnologyHesse and R. Wagner (2000), «Developments and improvements in the manufacturing of humanF.therapeutics with mammalian cell cultures.»Trends in Biotechnology, vol. 18, p.173.Hauser and R. Wagner (1997),Mammalian Cell Biotechnology in Protein Production. WalterdeH.3-11-013403-9.Gruyter,Wolfe (1993) Mediafor Cell Culture in Biotechnology Second, Completely Revised Edition, 141.R.3, G. Stephanopoulos (ed.). VCH Verlagsgesellschaft, ISBN 3-527.28313-7.Vol.Wirth and H. Hauser (1993) Genetic Engineering of Animal Cells in Biotechnology Second,M.Revised Edition, 663. Vol. 2, A Piihler (ed.). VCH Verlagsgesellschaft, ISBN 3-CompletelyChu and D. K. Robinson (2001), «Industrial choices for protein production by large-scale cellL.Current Opinion in Biotechnology, vol. 12, 180.culture.»S. Hu and J. G. Aunms (1997), «Large-scale mammalian cell culture.» Current Opinion in Biotechnology,W.vol. 8, p. 148.Sambanis and W. Hu (1993) Cell Culture Bioreactors in Biotechnology Second, Completely RevisedA.Edition, 105. Vol. 3, G. Stephanopoulos (ed.). VCH Verlagsgesellschaft, ISBN 3-527-Bliem, K. Konopitzky and H. Katinger (1991), «Industrial animal cell reactor systems: aspectsR.and evaluation.» Advances in Biochemical Engineering/Biotechnology, vol. 44, p. 1ofselectionBommarius (1993), Biotransformations and Enzyme Reactors in Biotechnology Second, CompletelyA.Revised Edition, 7. Vol. 3, G. Stephanopoulos (ed.). VCH Verlagsgesellschaft, ISBN 3-Furusaki and M. Seld (1992), «Use and engineering aspects ofimmobilized cells in biotechnology.»S.Advances in Biochemical Engineering/Biotechnology, vol. 46, p.161.?fGQR9*;LJ9+*ODyGCultivation of mammaliam cells527-28312-99*;LJ9+*ODyG?*(+AGJ;f9q|Mammalian cell's bioreactorsG. Kretzmer (2002), «Industrial processes with animal cells.»Applied Microbiology and Biotechnology,vol. 159, p. 135.Kelley, T. Chiou, M. Rosellberg and D. Wang (1993) Industrial Animal Cell Culture in BiotechnologyB.Second, Completely Revised Edition, 23. Vol. 3, GStephanopoulos (ed.). VCH Verlagsgesellschaft,ISBN 3-527-28313-7.Aunins and H. Henzler (1993).Oxygen Transfer in Cell Culture Bioreactors in Biotechnology Second,J.Completely Revised Edition, 219. Vol 3, G Stephanopoulos (ed.). VCH Verlagsgesellschaft,ISBN 3-527-28313-7.28313-7.?+}*R!}G?*(zBGhEnzyme and cell reactorsJ;f9qCG527-283137.425

Rudolph, H. Lilie and E. Schwarz (1999) In vitro Folding of Inclusion Body Proteins on an IndustrialR.Scale in Biotechnology Second, Completely Revised Edition, 111. Vol. 5a, U. Ney,Mukhopadhyay (1997), «Inclusion bodies and purification of proteins in biologically activeA.Advances in Biochemical Engineering/Biotechnology, vol. 56, p. 61.forms.»(1993). Overwiev of Downstream Processing in Biotechnology Second, Completely RevisedR.SpearsEdition, 39, Vol. 3, G Stephanopoulos (ed.). VCH Verlagsgesellschaft, ISBN 3-527-and J. Henry (1988) Separation in Biotechnology;Biochemical Separations in Ullmann'sJ.Shaeiwitzof Industrial Chemistry 5 th edition B3, 11. WileyVCH, ISBN 3-527-20133-5.EncyclopediaBurgessn and N. Thompson (2002), «Advances in gentle immunoaffinity chromatography.»R.Opinion in Biotechnology, vol. 13, p. 304.CurrentImamoglu (2002), «Simulated moving bed chromatography (SMB) for application in bioseparation.»S.Advances in Biochemical Engineering/Biotechnology, vol. 76,p. 211.Svec (2002), «Capillary electrochromatography: a rapidly emerging separation method.» AdvancesF.in Biochemical Engineering/Biotechnology, vol. 76, p. 1.A. Queiroz, C. T. Tomaz, J. M. Cabral (2001), «Hydrophobic interaction chromatography ofJ.Journal of Biotechnology, vol. 87, p. 143.proteins.»King (1999) Use ofAntibodies for Immunopurification in Biotechnology Second, Completely RevisedD.Edition, 275. Vol. 5a, U. Ney, D. Schomburg (ed.), VCH Verlagsgesellschaft, ISBN 3-Ladisch (1998) Bioseparations in Kirk-Othmer Encyclopediaof Chemical Technology 4 th EditionM.Supplement, 89Interscience-Wiley, ISBN 0-47152696-7Labrou and Y. D. Clonis(1994), «The affinity technology in dowllstream processing.» JournalN.Biotechnology, vol. 36, p. 95.ofM. Boyer and J. T.Hsu (1993), «Protein purification by dye-ligand chromatography.» AdvancesP.Biochemical Engineering/Biotechnology, vol. 49, p. 1.inD. Diamond and J. T.Hsu (1992), «Aqueous two-phase systems for biomolecule separation.»A.in Biochemical Engineering/Biotechnology, vol. 47, p.89.AdvancesJagschies (1988). Separation in Biotechnology: Process-Scale Chromatography in Ullmann's EncyclopediaG.of Industrial Chemistry 5 th edition B3, 10. WileyVCH, ISBN 3-527-20133-5.Rehmann (1996). Nucleic Adds in kirk-Othmer Encyclopedia of Chemical Technology 4 th EditionJ.17, 507. Interscience-Wiley, ISBN 0-471-52686-XRecovery of bioproductsY9FQASGJ9GA"CG?*(+AGD.Schomburg (ed.)» VCH Verlagsgesellschaft, ISBN 3-527-283153.28313-7.Recovery of bioproducts : chromatographyJ9GA"CG:?*(+AG9+pGQi(@9|hQwyGY9FQASG527-28315-3.DNA structure:DNA?+"=yGyv G426

GoÈ tz (1993) Structure and Function of DNA in Biotechnology Second, Completely Revised Edition,F.191. Vol. 2, A PuÈ hler (ed.). VCR Verlagsgesellschaft, ISBN 3527-28312-9Schmidt (1995), Genetic Engineering, Procedures in Kirk-Othmer Encyclopedia of ChemicalF.4 th Edition 12, 440. Interscience-Wiley, ISBN 0-47152681-9.TechnologyNagarajan (1994), Genetic Engineering, Microbes in Kirk-Othmer Encyclopedia of ChemicalV.4 th Edition 12,481, Interscience-Wiley, ISBN 0-471-52681-9.TechnologyHolloway (1993) Genetic Exchange Processes for Prokaryotes in Biotechnology Second, CompletelyB.Revised Edition, 47. Vol. 2, A. PuÈ hler (ed.). VCH VerlagsgeseUschaft, ISBN 3-527-Stahl and K. Esser (1993) Genetic Exchange Processes in Lower Eukaryotes in BiotechnologyU.Completely Revised Edition, 73. Vol. 2, A PuÈ hler (ed.). VCH Verlagsgesellschaft,Second.L. Ong and A. Irvine (2002). «Quantitative realtime PCR: a critique of method and practicalY.vol.7, p.59.considerations.»Hematology,A. Erlich, D. Gelfand and J. J. Sninsky (1991), «Recent advances in the polymerase chain reaction.»H.Science, vol.252, p. 1643.Middendorf, P. Humphrery, N. Narayanan, S. Roemer (2001),Sequencing Technology in BiotechnologyL.2 nd Edition 193. Vol. 5b, C. Sensen (ed.). VCH-Wiley, ISBN 3-527-28328-5.Volckaert, P. Verhasselt, M. Voet and J. Robben (1993),DNA Sequencing in Biotechnology Second,G.Completely Revised Edition, 257. Vol. 2. A PuÈ hler (ed.). VCH Verlagsgesellschaft,Schwab (1993). Principles of Genetic Engineering for Escherichia coli in Biotechnology Second,H.Revised Edition, 373, Vol. 2, A PuÈ hler (ed.). VCH Verlagsgesellschaft, ISBN 3-CompletelyPriefer (1993), Principles of Genetic Engineering of Gram-negative Bacteria in BiotechnologyU.Completely Revised Edition, 427. Vol. 2, A. PuÈ hler (ed.). VCH Verlagsgesellschaft,Second.Sudbery (1993),Genetic Engineering of Yeast in Biotechnology Second, Completely Revised Edition,P.507. Vol. 2. A. PuÈ hler (ed.). VCH Verlagsgesellschaft, ISBN 3-527-28312-9.:?+CGQ(yG?SO"%yG?|9fJG(aBGGenetic engineering: general steps28312-9.ISBN 3-527-283129.RGQ}+y(=yG:\TzTAyG?t*QayG ?|9gyG{f9q@PCR: general methodyv G?zTzSDNADNA sequencingISBN 3-52728312-9.{t!DNA?+AG9*;BG]E>*QjyGyv GTransfer of foreign DNA in living cells (transformation)527-283129.Wohlleben, G. Muth and J. Kalinowski (1993) Genetic Engineering of Gram-Positive BacteriaW.Biotechnology Second. Completely Revised Edition, 455. vol. 2, A. PuÈ hler (ed.). VCH Ver-inlagsgesellschaft, ISBN 3-527-28312-9.ISBN 3527-28312-9.427

Turner (1993), Genetic Engineering of Filamentous Fungi in Biotechnology Second, CompletelyG.Edition. 529, Vol. 2, A. PuÈ hler(ed.). VCH Verlagsgesellschaft,ISBN 3-527-28312-9.RevisedGorman and C. Bullock (2000), «Site-specific gene targeting for gene expression in eukaryotes.»C.Opinion in Biotechnology, vol. 11, p. 455.CurrentBaneyx (1999), «Recombinant protein expression in Escherichia coli.» Current Opinion in Biotechnology,F.vol. 10, p.411.Mattes (1993), Principles of Gene Expression in Biotechnology Second, Completely Revised Edition,R.233. Vol. 2, A PuÈ hler (ed.). VCH Verlagsgesellschaft, ISBN 3527-28312-9.Fatica and D. Tollelvey (2002), «Making ribosomes.»Current Opinion in Biotechnology, vol.14,A.313. p.A. Doudna and T. R. Cech (2002), «The chemical repertoire ofnatural ribozymes.»Nature,J.418, p. 222.vol.A. Sullenger and E. Gilboa (2002). «Emerging clinical applications of RNA.» Nature, vol.418,B.252. p.Hermann and D. J. Patel (2000). «Adaptive recognition by nucleic acid aptamers.» Science,T.p. 820.vol.287,Famulok, G. Mayer and M. Blind (2000), «Nucleic acid aptamers-from selection in vitro to applicationsM.in vivo.» Accounts of Chemical Research, vol. 33, p. 591Sterky and J. Lundeberg (2000). «Sequence analysis of genes and genomes.» Journal of Biotechnology,F.vol. 76, p. 1.PuÈ hler, D.Jording, J. Kalinowski, D. Buttgereit, R. Renkawitz-Pohl, L. Altschmied, A. Danchin,A.H. Feldmann, H. KJeink and M. Kroger (2001),Genome Projects of Model OrganismsTsui and S. Scherer (2001) The Human Genome Project in Biotechnology 2 nd Edition, 41.L.5b, C. Semen (ed.). VCR-Wiley, ISBN 3-527-28328-5.Vol.,CGQ(yGQ+=gAyGGene expressionGene silencingJ9"+@GJ9wSCS. W. Ding (2000), «RNA silencing.» Current Opinion in Biotechnology, vol. 11,p.152.RNARNAyvGG. F.Joyce (2002). «The antiquity of RNAbased evolution.» Nature, vol. 418, p. 214.G. J. Hannon (2002), «RNA interference.» Nature, vol. 418, p. 244.Gene libraries and gene mappingbFGQBGh?+"+@G ?+"+@GJ9=AwCGGeneomes of prokaryotes and eukaryotes979+t+tIh?+"+@G J9+yh} i("yGbFGQBGin Biotechnology 2 nd Edition, 5. Vol. 5b,C Sensen (ed.). VCH-Wiley, ISBN 3-527-28328-5.The human genomejQW=yGe("+@G428

Green (2001), Genomics and Human Disease in Biotechnology 2 nd Edition, 105. Vol. 5b, C. SensenR.(ed.). VCH-Wiley, ISBN 3-527-28328-5.Dellaire (2001), Genetic Disease in Biotechnology 2 nd Edition, 61. Vol. 5b, C Sensen (ed.).G.ISBN 3-52728328-5.VCH-Wiley,M. Hurley and C. H. Rodeck (1997), Prenatal diagnosis in Molecular Biologyin Medicine 299.P.M. Cox and J. Sinclair (ed.). Blackwell Science Ltd., ISBN 0-632-02785-1.T.D. Shoemaker and P. S. Linsley (2002), «Recent developments in DNA mictoarrays.» CurrentD.in Microbiology, vol. 5, p. 334.OpinionF. Templin, D. Stoll and M. Schrenk [et al.] (2002), «Protein microarray technology.» TrendsM.vol. 20, p. 160.inBiotechnology,Eickhoff, Z. Konthur and A. Lueking [et al.] (2002), «Protein array technology: the tool toH.genomics and proteomics.» Advances in Biochemical Engineering/Biotechnology,bridgeH. Blohm and A. Guiseppi-Elie (2001), «New developments in microarray technology.» CurrentD.in Biotechnology, vol. 12, p. 41.OpinionTessier, D. Thomas and R. Brousseau (2001).ADNA Microarrays Fabrication Strategy in BiotechnologyD.2 nd Edition, 227. Vol. 5b, C. Sensen (ed.). VCH-Wiley, ISBN 3527-28328-5.T. Fung, V. Thulasiraman, S. R. Weinberger and E. A. Dalmasso (2001), «Protein biochips forE.profiling.» Current Opinion in Biotechnology, vol. 12, 65.differential-L. van Hal, O. Vorst, A. M. van Houwelingen, E. J. Kok, A. Peijnenburg, A Aharoni, A. J. vanN.J. Keijer (2000). The application of DNA micro arrays in gene expression analysis.»-Tunen,Lueking, M Horn, and H. Eickhoff [et al.] (1999), «Protein microarrays for gene expression andA.screening.» Analytical Biochemistry,vol. 270, p. 103.antibodyRigler (1995), «Fluorescence correlations, single molecule detection and large number screening,R.Application in biotechnology.» Journal of Biotechnology, vol. 41, p. 177.Zhao, K. Chockalingam and Z. Chen (2002), «Directed evolution of enzymes and pathways forH.biocatalysis.» Current Opinion in Biotechnology, vol. 13, p. 104.industrial,q+c(yG{+zJAyGFunctional analysis of the human genomejQW=yGe("+GzyMange, A. Mange (1994) Basic Human Genetics. Sinauer Associates, ISBN 0-87893495-2.DNADNA assaysyvGJGQ*9g|DNADNA and protein arraysyvGh#+@hQ=yGJ9p(qZ|vol. 77, p. 103.Journal of Biotechnology, vol. 78, p. 271.M. Schena (2000), Microarray Technology, Eaton Publishing. ISBN 1-881299-37-6.M. Hagmann (2000), «Doing Immunologyon a chip.» Sciences, vol. 290, p. 82.IQ=MCGJ9f(}GCGReporter groups,"+@hQ=yG~+}ZAyGProtein design429

van den Burg and V. Eijsink (2002), «Selection ofmutations for increased protein stability.»B.Opinion in Biotechnology, vol. 13, p. 333.CurrentG. Illing, H Oschkinat (2001), «High-throughput three-dimensional protein structureU.Heinemann,determination.» Current Opinion in Biotechnology, vol. 12, p. 348.Wahler and J. L. Reymond (2001), «Highthroughput screening for biocatalysts.»Current OpinionD.in Biotechnology, vol. 12,p.535.Wu and M. M. Ataai (2000), «Production ofviral vectors for gene therapyapplications.»CurrentN.Opinion in Biotechnology, vol. 11, p. 205.Mountain (1999), Overview of Gene Therapy in BiotechnologySecond, CompletelyRevised Edition,A.383, Vol. Sa, U. Ney, D. Schomburg (ed.). VCH Verlagsgesellschaft, ISBN 3-S27-Carter (1999) Viral Vectors for Gene Therapyin BiotechnologySecond, CompletelyRevised Edition,B.39S. Vol. Sa, U.Ney, D. Schomburg (ed.). VCH Verlagsgesellschaft, ISBN 3-527-28315-Weir (1999) Non-Viral Vectors for Gene Therapy in BiotechnologySecond, CompletelyRevisedN.427. Vol. Sa, U.Ney, D. Schomburg (ed.).VCH Verlagsgesellschaft, ISBN 3-527-Edition,M. L. Lever (1997). Gene therapy in Molecular Biologyin Medicine, 284. T. M Cox, J. SinclairA.Blackwell Science Ltd., ISBN 0-632-02785-1.(ed.).Burgess and B. Witholt (2002), «Protein research proteomics and applied enzymology.» CurrentR.in Biotechnology, vol. 13, p. 289.OpinionDovichi, S. Ru, D. Michels, Z. Zhang, S. Krylov (2001). Proteome Analysis by Capillary ElectrophoresisN.in Biotechnology2 nd Edition, 269. Vol. 5b. C. Sensen (ed.). VCH-Wiley, ISBN 3-Figeys (2001), Two-Dimensional Gel Electrophoresis and Mass Spectrometry for Proteomic Studies:D.State-of the-Art in Biotechnology 2 nd Edition, 241. Vol. 5b. C Sensen (ed.). VCH-Wiley,F. Leung and C. P. Pang (2001), «Trends in proteomics.» Trends inBiotechnology, vol. 19,Y.480. p.A. Raynes and J. R. Yates, 3rd (2000). «Proteome, profiling-pitfalls and progress.» Yeast,P.17, p.81.vol.L;gyG,"+@GGene therapyA. Mountain (2000), «Gene therapy: the first decade.» Trends in Biotechnology, vol.18, p.119.28315-3.3.28315-3e(+@hQ=yG?SGQOProteomics527-28328-5.ISBN 3-52728328-5.P. Gygi, G. L. Corthals, Y. Zhang, Y. Rochon and R. Aebersold (2000), «Evaluation of twodimensionalS.gel electrophoresis-based proteome analysis technology.» Proceedings of the NationalAcademy of Sciences, vol.97, p. 9390.430

F. Lopez (2000), «Better approaches to finding the needle in a haystack: optimizing proteomeM.through automation.»Electrophoresis, vol.21, p.1082.analysisKellner, F. Lottspeich and R. Meyer (1999) Microcharacterization ofProteins, 2 nd , Wiley VCR.R.3-527-30084-8.ISBNLottspeich (1999). «Proteome Analysis: A Pathway to the Functional Analysis of Proteins.» AngewandteF.Chemie International Edition, vol. 38, p. 2476.Hansson, C. Oostenbrink, W. van Gunsteren (2002), «Molecular dynamics simulations.»CurrentT.Opinion in Structural Biology, vol. 12, p. 190.N. Kinch and N. V.Grishin (2002). «Evolution of protein structures and functions.»CurrentL.in Structural Biology, vol. 12, p.400.OpinionNorin and M Sundstrom (2002). «Structural proteomics: developments in structure-to-functionM.Trends inBiotechnology, vol. 20, p. 79.predictions.»Saven (2002), «Combinatorial protein design.» Current Opinion in Structural Biology, vol.12,p.J.453.Reiss (2001). «Drug discovery of the future: the implications of the human genome project.»T.in Biotechnology, vol. 19, p. 496.TrendsRamanathan and D. Davison (2001),Pharmaceutical Bioiriformatics and Drug Discovery in Biotechnology.C.2 nd Edition, 123.Vol. 5b. C Sensen (ed.). VCH-Wiley, ISBN 3527-28328-5.N. Kyranos, H. Cai, D Wei, W. K. Goetzinger (2001), «High-throughput high-performance liquidJ.chromatography/mass spectrometry for modern drug discovery.» Current Opinion inM. Lawn and L. A. Lasky (2000), «Phannaceutical biotechnology: The genomes are just the beginning.»R.Current Opinion in Structural Biology, vol. 11, p. 579.Gregersen (1995),Biomedicinal Product Development in Biotechnology 2 nd Edition, 213. Vol. 12,J.Brauer (ed.). VCRWiley, ISBN 3-527-28322-6D.Rice (2001), Bioinformatics: Tools for DNA Technologies in Biotechnology 2 nd Edition, 61.P.5b, C. Sensen (ed.). VCHWiley, ISBN 3-527-28328-5.Vol.Wishart (2001),Bioinformatics: Tools for Protein Technologies in Biotechnology 2 nd Edition,D.Vol. 5b, C. Sensen (ed.). VCH Wiley, ISBN 3-527-28328-5.325.Zdobnov, R. Lopez, R. Apweiler and T. Etzold (2001), Bioinformatics: Using the Molecular.E.Data in Biotechnology 2 nd Edition, 281. Vol. 5b, C. Sensen (ed.). VCHWiley, ISBNBiologyCygler, A. Matte and J. Schrag (2001),Bioinformatics: Structure Information in BiotechnologyM. nd Edition, 345. Vol. 5b, C. Sensen (ed.); VCR-Wiley, ISBN 3-52728328-5.2Poetzsch (1995), Databases in Biotechnology in Biotechnology 2 nd Edition, 323. Vol. 12, D.E.(ed.). VCH-Wiley, ISBN 3-527-28322-6.BrauerDrug screening?*hO}G?z

Kramer and G. Sprenger (1993) Metabolism in Biotechnology Second, Completely Revised Edition,R.50. Vol. 1, H. Sahm (ed.). VCR Verlagsgesellschaft, ISBN 3-527-28337-4Christensen and J. Nielsen (2000), «Metabolic network analysis: A powerful tool in metabolicB.Advances in Biochemical Engineering/Biotechnology, vol. 66, p. 209.engineering.»Fotheringham (1999), Engineering Microbial Pathways for Amino Acid Production in Biotechnology,I.2 nd Edition, 313, Vol. 8a, D. Kelly (ed.). VCH-Wiley, ISBN 3-527-30104-6.N. Stephanopoulos, A. A. Aristidou, J, Nielsen (1998), Metabolic Engineering -Principles andG.Academic Press, ISBN 0-12-666260-6.Methodologies,Hansen and M. C. Kielland-Brandt (1996), «Modification of biochemical pathways in industrialJ.Journal of Biotechnology, vol. 49, p. 1.yeasts.»Sahm (1993). Metabolic Design in Biotechnology Second, Completely Revised Edition, 189.H.1, H. Sahm (ed.). VCH Verlagsgesellschaft, ISBN 3-52728337-4.Vol.M. Loew, J. C. Schaff (2001), «The Virtual Cell: a software environment for computational cellL.Trends inBiotechnology, vol. 19, p. 401.biology.»L. Simpson, G. S. Sayler, J.T.Fleming, B. Applegate (2001), «Whole-cell biocomputing.»M.inBiotechnology, vol. 19, p. 317.TrendsDroge, A. PuÈ hler and W. Selbitschka (1998), «Horizontal gene transfer as a biosafety issue: aM.phenomenon of public concern.» Journal of Biotechnology, vol. 64, p. 75.naturalBrauer, M. Broker, C. Kellermann and E. Winnacker (1995),Biosafety in rDNA Research andD.in Biotechnology 2 nd Edition, 63. Vol. 12, D. Brauer (ed.). VCH-Wiley, ISBN 3-ProductionMedley and S. McCammon (1995). Strategic Regulations for Safe Development of TransgenicT.in Biotechnology 2 nd Edition, 197. Vol. 12, D. Brauer (ed.). VCH Wiley, ISBN 3-527-PlantsSimon and W Frommer (1993) Safety Aspects in Biotechnology in Biotechnology Second, CompletelyR.Revised Edition. 835. Vol. 2, A. PuÈ hler (ed.). VCH Verlagsgesellschaft, ISBN 3-527-Hasskarl, R. Kretzschmar, K-J. Hahn and M. Zahn (1991) Pharmaceuticals, General SurveyH.Development in Ullmann's Encyclopedia of Industrial Chemistry 5 th edition A19, 273.and^*}G--H;tAS:GMetabolismG. Michal (1999), Biochemkal Pathways, Spelctrum Akademischer Verlag. ISBN 386025-239-9.Metabolic engineering?+]*}G?SO"%yG~zfSystem biology~d"yG )9+F(y(+

J. Dale (1999), «Public reactions and scientific responses to transgenic crops.» Current OpinionP.Structural Biology, vol. 10, p. 203.inE. Spier (1998), «Animal and plant cell technology: a critical evaluation of the technology/societyR.interface.» Journal of Biotechnology, vol. 65, p. 111.Huttner (1995) Government, Researchers and Activists: The Critical Public Policy Inteiface inS.2 nd Edition, 459. Vol. 12, D. Brauer (ed.). VCH-Wiley, ISBN 3-527-28322-6.BiotechnologyWeise, H. Friege, G. Altner, P. Schmitz and K. Klostermaier (1995) Ethics and Industrial ChemistryE.in Ullmann's Encyclopedia of Industrial Chemistry 5 th edition B7, 1. Wiley-VCH, ISBNKepplinger and S. Ehmig (1995),Press Coverage of Genetic Engineering in Germany; Facts,H.and Causes in Biotechnology, 2 nd Edition, 495. Vol. 12, D. Brauer (ed.). VCH-Wiley,FaultsSzarka (1999), «Patenting in biotechnology: a review of the 20 th symposium of ECB8.» JournalEBiotechnology, vol. 67, p.1.ofGresens (1996), Patents and Trade Secrets in Kirk-Othmer Encyclopedia of Chemical TechnologyJ.4 th Edition 18, 61 Interscience-Wiley, ISBN 0-471-52687-8.Straus (1995),Biotechnology and Intellectual Property in Biotechnology 2 nd Edition, 281.J.12,D. Brauer (ed.). VCH-Wiley, ISBN 3-527-28322-6.Vol.Gregersen (1995) Patent Applications for Biomedicinal Products in Biotechnology 2 nd Edition,J.Vol. 12, D. Brauer (ed.). VCH-Wiley, ISBN 3-527-28322-6299.Hauf (1990) Patents in Ullmann's Encyclopedia of Industrial Chemistry 5 th edition Bl, 13. Wiley-VCHW.ISBN 3-52720131-9.Schmid, F. Hollmann, J. Park and B. Buhler (2002). «The use of enzymes in the chemical industryA.in Europe.» Current Opinion in Structural Biology, vol. 13, p. 359.&Young (2002), Beyond Borders -The Global Biotechnology Report 2000. Ernst &YoungErnst.2002,&Young (2001), Integration-Ernst &Young's Eigth Annual European Life Science ReportErnst.2001,&Young (2001), Focus on Fundamentals -The Biotechnology Report. .JGQ9=Af:G?+s;L}Gd(=tyGhEthical considerations and acceptanceD. Macer (1995) Biotechnology and Bioethics: What is Ethical Biotechnology? in Biotechnology 2 ndEdition, 115. Vol. 12, D. Brauer (ed.). VCH-Wiley, ISBN 3-52728322-6.3-527-20137-8.ISBN 3-527-28322-6.?*(+AGYGQAL:G ,p ?!9tAyGJGAGQ

&Young (2000),Convergence: The Biotechnology Industry Report, Millenium Edition,Ernst.Beppu (2000). «Development of applied microbiology to modern biotechnology in Japan.» AdvancesT.in Biochemical Engineering/Biotechnology, vol. 69, p. 41.Schmid, B. Chung, A. Jones, S. Saono, J. Scriven and J. Tsai (1995), Biotechnology in the Asian-R.Region in Biotechnology 2 nd Edition, 369, Vol. 12, D. Brauer (ed.). VCH-Wiley,PacificJSBN 3-527-28322-6.434

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