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Cross-linking125I-GF125 I-GF125 I-GF125 I-GF 125 I-GF125 I-GF125 I-GFY Y Y Y Y Y YY Y Y Y Y YX125 I-GF125 I-GF125 I-GF 125 I-GF125 I-GF125 I-GFY Y Y Y Y Y YY Y Y Y Y YBis(Sulfosuccinimidyl)suberate (BS 3 ) is a homobifunctional, water-soluble, non-cleavable and membraneimpermeable cross-linker. It contains an amine-reactive N-hydroxysulfosuccinimide (NHS) ester at eachend of an 8-carbon spacer arm. NHS esters react with primary amines at pH 7-9 to form stable amidebonds, along with release of the N-hydroxysulfosuccinimide leaving group. Proteins, including antibodies,generally have several primary amines in the side chain of lysine (K) residues and the N-terminus of eachpolypeptide that are available as targets for NHS-ester cross-linking reagents.http://www.piercenet.com/Products/Browse.cfm?fldID=02030212