ALESSIO PAFFONI BIOGRAPHICAL SKETCH 21st December, 2012 ...

ALESSIO PAFFONI BIOGRAPHICAL SKETCH 21 st December, 2012PERSONAL DATADATE AND PLACE OF BIRTH: 10 January 1978, Borgomanero Italy.CITIZENSHIP: ItalianWORK ADDRESS: Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico, Infertility Unit, viaM. Fanti 6, MilanCONTACT: phone 00390255034305 email: alessio.paffoni@alice.itEDUCATIONINSTITUTION AND LOCATION DEGREE YEAR FIELD OF STUDYUniversity of Milan, Italy M.Sc. 2006 Medical GeneticsUniversity of Milan Bicocca, Italy M.Sc. 2012 BiologyUniversity of Milan, Italy M.Sc. 2002 BiotechnolgyRESEARCH AND PROFESSIONAL EXPERIENCEFondazione Ospedale Maggiore Policlinico Mangiagalli e Regina Elena. University of Milan, Italy2002-2012. Embryology. In vitro Fertilization and micromanipulation (IVF, ICSI, MESA, TESE).Gametes cryopreservation: animal models. Parthenogenetic activation of human oocytes.2000-2009. Cryobiology. Ovarian tissue cryopreservation; male and female gametes cryopreservation.SPECIFIC TRAINING2002 December. Training on Ovarian tissue cryopreservation and follicle culture. ClarendonLeeds University.2009 January. Training on First Polar Body PGD. Reproductive Genetics Institute, Chicago.2009 March. Training on First Polar Body PGD. University of Lubeck, Lubeck.AWARDS First prize for poster presentation at American Society of Reproductive Medicine (ASRM),Annual Meeting 2006, New Orleans, with the poster “Assessment of human oocytesdevelopmental competence by in vitro culture following parthenogenetic activation.” “Senior Embryologist” of the European Society for Human Reproduction and Embryology(ESHRE), 2008.PUBLICATIONS (selection)1. Benaglia L, Somigliana E, de Benedictis S, Paffoni A, Scarduelli C, Fedele L, Ragni G.Hyperstimulation during IVF cycles does not modify dimensions of small subserosal and intramuralleiomyomas. Fertil Steril. 2011 Jun 30;95(8):2489-91. Epub 2011 Jan 14. PubMed PMID:21236423.2. Brevini TA, Pennarossa G, Antonini S, Paffoni A, Tettamanti G, Montemurro T, Radaelli E, LazzariL, Rebulla P, Scanziani E, de Eguileor M, Benvenisty N, Ragni G, Gandolfi F. Cell lines derivedfrom human parthenogenetic embryos can display aberrant centriole distribution and alteredexpression levels of mitotic spindle check-point transcripts. Stem Cell Rev. 2009 Dec;5(4):340-52.Epub 2009 Sep 9. PubMed PMID: 20058199.3. Brevini TA, Pennarossa G, deEguileor M, Tettamanti G, Ragni G, Paffoni A, Gandolfi F.Parthenogenetic cell lines: an unstable equilibrium between pluripotency and malignanttransformation. Curr Pharm Biotechnol. 2011 Feb 1;12(2):206-12. Review. PubMed PMID:21044006.

4. Brevini TA, Vassena R, Paffoni A, Francisci C, Fascio U, Gandolfi F. Exposure of pig oocytes toPCBs during in vitro maturation: effects on developmental competence, cytoplasmic remodellingand communications with cumulus cells. Eur J Histochem. 2004 Oct-Dec;48(4):347-56. PubMedPMID: 15718200.5. Carmignani L, Gadda F, Gazzano G, Ragni G, Paffoni A, Rocco F, Colpi GM. Testicular spermextraction in cancerous testicle in patients with azoospermia: a case report. Hum Reprod. 2007Apr;22(4):1068-72. Epub 2006 Dec 15. PubMed PMID:17172283.6. Carmignani L, Gadda F, Paffoni A, Bozzini G, Stubinsky R, Picozzi S, Rocco F. Azoospermia andsevere oligospermia in testicular cancer. Arch Ital Urol Androl. 2009 Mar;81(1):21-3. PubMedPMID: 19499754.7. Cillo F, Brevini TA, Antonini S, Paffoni A, Ragni G, Gandolfi F. Association between human oocytedevelopmental competence and expression levels of some cumulus genes. Reproduction. 2007Nov;134(5):645-50. PubMed PMID: 17965254.8. Ciriminna R, Papale ML, Artini PG, Costa M, De Santis L, Gandini L, Parmegiani L, Ragni G,Revelli A, Rienzi L; Italian Society of Embriology, Reproduction and Research, Barbaro R, Cela V,Cino I, Colia D, D'Ambrogio G, Diotallevi L, Dusi M, Filicori M, Genazzani AR, Giuffrida G,Lombardo F, Paffoni A, Racca C, Greco E. Impact of Italian legislation regulating assistedreproduction techniques on ICSI outcomes in severe male factor infertility: a multicentric survey.Hum Reprod. 2007 Sep;22(9):2481-7. Epub 2007 Jun 23. PubMed PMID: 17586834.9. Doni A, Paffoni A, Nebuloni M, Ragni G, Pasqualini F, Valentino S, Bonetti S, Mantovani A,Somigliana E, Garlanda C. The long pentraxin 3 is a soluble and cell-associated component of thehuman semen. Int J Androl. 2009 Jun;32(3):255-64. Epub 2007 Dec 30. PubMed PMID: 18179560.10. Ferrari S, Lattuada D, Paffoni A, Brevini TA, Scarduelli C, Bolis G, Ragni G, Gandolfi F. Procedurefor rapid oocyte selection based on quantitative analysis of cumulus cell gene expression. J AssistReprod Genet. 2010 Jul;27(7):429-34. Epub 2010 May 14. PubMed PMID: 20467802; PubMedCentral PMCID: PMC2922707.11. Gadda F, Spinelli MG, Cozzi G, Paffoni A, Carmignani L, Rocco F. Emergency testicular spermextraction after scrotal trauma in a patient with a history of contralateral orchiopexy forcryptorchidism: case report and review of the literature. Fertil Steril. 2012 May;97(5):1074-7. Epub2012 Mar 9. PubMed PMID: 22405599.12. Gandolfi F, Paffoni A, Papasso Brambilla E, Bonetti S, Brevini TA, Ragni G. Efficiency ofequilibrium cooling and vitrification procedures for the cryopreservation of ovarian tissue:comparative analysis between human and animal models. Fertil Steril. 2006 Apr;85 Suppl 1:1150-6. PubMed PMID: 16616087.13. Natacci F, Baffico M, Cavallari U, Bedeschi MF, Mura I, Paffoni A, Setti PL, Baldi M, Lalatta F.Germline mosaicism in achondroplasia detected in sperm DNA of the father of three affected sibs.Am J Med Genet A. 2008 Mar 15;146A(6):784-6. PubMed PMID: 18266238.14. Paffoni A, Alagna F, Somigliana E, Restelli L, Brevini TA, Gandolfi F, Ragni G. Developmentalpotential of human oocytes after slow freezing or vitrification: a randomized in vitro study based onparthenogenesis. Reprod Sci. 2008 Dec;15(10):1027-33. PubMed PMID: 19088372.15. Paffoni A, Brevini TA, Gandolfi F, Ragni G. Parthenogenetic activation: biology and applications inthe ART laboratory. Placenta. 2008 Oct;29 Suppl B:121-5. Epub 2008 Sep 7. Review. PubMedPMID: 18778853.

16. Paffoni A, Brevini TA, Somigliana E, Restelli L, Gandolfi F, Ragni G. In vitro development ofhuman oocytes after parthenogenetic activation or intracytoplasmic sperm injection. Fertil Steril.2007 Jan;87(1):77-82. Epub 2006 Oct 30. PubMed PMID: 17074324.17. Paffoni A, Guarneri C, Ferrari S, Restelli L, Nicolosi AE, Scarduelli C, Ragni G. Effects of twovitrification protocols on the developmental potential of human mature oocytes. Reprod BiomedOnline. 2011 Mar;22(3):292-8. Epub 2011 Jan 26. PubMed PMID: 21269885.18. Paffoni A, Paracchini V, Ferrari S, Scarduelli C, Seia M, Coviello DA, Ragni G. Use ofparthenogenetic activation of human oocytes as an experimental model for evaluation of polar bodybased PGD assay performance. J Assist Reprod Genet. 2011 May;28(5):461-70. Epub 2011 Mar1. PubMed PMID: 21360136; PubMed Central PMCID: PMC3151362.19. Paffoni A, Ragni G, Doni A, Somigliana E, Pasqualini F, Restelli L, Pardi G, Mantovani A,Garlanda C. Follicular fluid levels of the long pentraxin PTX3. JSoc Gynecol Investig. 2006Apr;13(3):226-31. Epub 2006 Mar 3. PubMed PMID:16516502.20. Paffoni A., Restelli L., Guarneri C., Capitanio E., Posa A., Scarduelli C., Ragni G. Pregnanciesafter transfer of vitrified blastocysts derived from vitrified oocytes or embryos: a small case series.European Journal of Obstetrics & Gynecology and Reproductive Biology. In press21. Pennarossa G., Paffoni A., Ragni G., Gandolfi F., Brevini TAL. Parthenogenesis in mammals: prosand cons in pluripotent cell derivation. Cent Eur J Biol. 2011; 6(5):770-775. DOI: 10.2478/s11535-011-0047-322. Ragni G, Arnoldi M, Somigliana E, Paffoni A, Brambilla ME, Restelli L. Reproductive prognosis inmale patients with azoospermia at the time of cancer diagnosis. Fertil Steril. 2005 Jun;83(6):1674-9. PubMed PMID: 15950635.23. Ragni G, Somigliana E, Benedetti F, Paffoni A, Vegetti W, Restelli L, Crosignani PG. Damage toovarian reserve associated with laparoscopic excision of endometriomas: a quantitative rather thana qualitative injury. Am J Obstet Gynecol. 2005 Dec;193(6):1908-14. PubMed PMID: 16325591.24. Ragni G, Somigliana E, Restelli L, Salvi R, Arnoldi M, Paffoni A. Sperm banking and rate ofassisted reproduction treatment: insights from a 15-year cryopreservation program for male cancerpatients. Cancer. 2003 Apr 1;97(7):1624-9. PubMed PMID: 12655518.25. Rienzi L, Cobo A, Paffoni A, Scarduelli C, Capalbo A, Vajta G, Remohí J, Ragni G, Ubaldi FM.Consistent and predictable delivery rates after oocyte vitrification: an observational longitudinalcohort multicentric study. Hum Reprod. 2012 Mar 22. [Epub ahead of print] PubMed PMID:22442248.26. Somigliana E, Benaglia L, Vercellini P, Paffoni A, Ragni G, Fedele L. Recurrent endometrioma andovarian reserve: biological connection or surgical paradox? Am J Obstet Gynecol. 2011Jun;204(6):529.e1-5. Epub 2011 Mar 17. PubMed PMID: 21419388.27. Somigliana E, Vigano P, Abbiati A, Paffoni A, Benaglia L, Vercellini P, Fedele L. Perinatalenvironment and endometriosis. Gynecol Obstet Invest. 2011;72(2):135-40. Epub 2011 May 31.PubMed PMID: 21625060.