Biological_Microscope

-OLYMPUS®
INSTF}UCTIONS
CX2lLED
BIOLOGICAL MICROSCOPE
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NorE! This equipment has been tested and found to comply with the limits for a Class A dlgltal devlce,
pursuant to Part 15 of the FCC Plules. These limit§ are c]esignecl to provicle reasonable prolec`lc)n
against harmful Interference when the ec|uipment is operated in a oommerclal envlronment Thls
equipment generates, uses, and can radiate radio frequency energy and, if not installed and used in
accordance wltn the ln§tructlon mar`ual, may cause riarmful interference to radio communications.
Operation of this equipment ln a residential area is likely to cause harmful interference in which case
the user will be required to correct the interference at his own expense.
FCC WARNING: Changes or modifications not expressly approved by the party responsible for compliance
could void the user's authonty to operate the equipment

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111111-
LED (llght emlttlng dlode) safety wamlng
llie LED built in this product is basically eye-safe. However, direct vlewlng the light from the LED urn for extenclecl tlme
may cause damage to the eyes.
1. After tr\e equipment has been used in an observation of a specimen that is accompanied with a potential of
infection. clean the parts coming in contact with the soecjmen tci prevent infecticin.
• Moving this product is accc)mpanied w)th the risk of dropping the Specimen. Be sure to remove the specimen before
moving this product.
• ln case the specimen is damagecl by erroneous operation, promptly take the infection prevention measures.
2. Install the microscope on a sturdy, level table or bench.
3. Always use the AC adapter and power cord provided by Olympus. If no power cord is provided, please select the proper
power cord by referring to the section "PF}OPEF` SELECTION OF THE POWEF} SUPPLY COF`D" at the end of this lnstruclion
manual. If the proper power cord is not used, product safety performance cannot be warranted.
4. Always ensure that the groundlng tomlnal of the microscope and that of the wall outlet are properly cc)nnected. If the
eqiiipment is not grounded, Olympus can no longer warrant the electrical safety performance of the equipment.
5. After operation c)r in case of abnormality, be sure to cllsconnect the power cord Of the AC adapter from the conr`ector on
the microscope or from the wall power outlet
Safety Symbols
The following symbc)ls are found on the micro§cope. Study the meaning of the symbols and always Ljse the eqLiipment
in the safest po§§ible manner.
Symbol
A
I
a
Explanatlon
Before use, cEirefully read the instruction manual. Improper use could result in personal injury to
the user and/or damage to the equipment.
lndlcates that the main switch is ON
lndicates that the maln swltch is OFF

lMPolun\IT - Be sure to read this sectlon for safe use of the equlpment. - 1€
1 STANDARD COMPONENT UNITS
2 NOMENCLATURE
3 SuMMARy oF BFtlGHTHELD OBSERvrmoN PRocEDURE 6
4 DETAILED OBSERvrmoN PF]ocEDURE 7-11
a Turning the LED Illumination oN ....... 7 a placing specimen on the stage ............ 7
a #:#::::g}::tser .... 8 a AdlustingthelnterpupillaryDistanee 9
d Ad]u§tlng the condenser po8[tlon and Aperture Iris Dlaphragm .,.......... ```... 10
a switching the objectives 10 a uslng the l00X lmmersl;;"6'b;.;.;i'lv;...:": .11
5 ONE-POINTADVICE
12
6 TF]ouBLESHoormNG GuiDE
13,14
7 SPECI FICATIONS
15
8 OpTicAL crIARAorEF`isTics
16
9 A§SEMBIY
17
10 OPTIONAL ACCESSORIES
18-20
10-1 System Diagram of Optional Accessories
10-2 Installation and operation of optional Accessories ............... 18
a Filter Holder CH2-FH and Darkfield Ping CH2-D
a F3eflection Mirror CH20-MM
a Eyepleces WHC15X/WHC15X-H
a using the eyepiece micrometer.
I PF]OPEF` SELECTION 0F "E POWEF` SUPPLY COF]D

CX21LED
a
Getting
f}eady
1. A mlcroscope is a precision Instrument. Handle it with care and avoid
subjecting it to suclden or severe impact
2. Do not use the microscope where it is subjected to direct sunlight,
high temperature anc] humidity, dust or vlbrations (For the operating
conditions, see chapter 7 nspECIFICATIONS" on Page 15)
3 Always use the tension adiustment ring to adiu§t the rotation tension cif
the coarse adjustment knob
4. Be sure to leave enough space around the AC adapter when installing
the microscope.
5. When canying the microscope, first make sure that the AC adapter has
been disconnected and hold both sicles around the hole of the arm as
shown in Pig 1 and cany carefully.
* To prevent damage, do not hold the microscope by the Stage ® or
observation tube ©.
Be sure to remove the specimen; othen^/ieo, It may fall.
*Do not carry the microscope with connecting the AC
adapter to prevent the damage to the AC adapter or
the microscope by hittlng the output connector of
the AC adapter.
*Be sure to disconnect the AC adapter from the microscope
when storing the mlcroscope after use.
a Maintenance and Storage
1. To clean the lenses and other glass componems, slmply blow dirty away using a Gommerclally avallable blower and wipo
gently uslng a plece of cleanlng paper (or clean gauze)
lfalenslsstainedwthflngeprlntsorollsmudge§,wipeilgauzeslightlymolstenedwthcommcrciallyavallableabsolute
alcohol
Aslnce the absolute alcohol is hlghly flammable, lt must be handled carefully.
Be sure to keep it away from Open flames or potential sources of electrical sparks - for example, electrical
equipment that ls belng switcnecl on or off. 6lnco 6uc,h operation may couge ignltlon ol a flro.
Also remember to always use it only in a well-ventllated room.
2. Do not attempt to use organic §olvent§ to clean the microscope components other than the glass components To clean
thorn, ueo a lint_free, eoft Cloth eliohtly mni§tEineirl with a dilutefl nButral deteraent.
3 Do not dlsassemble any part of the mlcroscope as this could result in malfunctlon or reduced performance.
4 When not using the microscope, store it in a dry locker or cover it with a dust cover
5. To clean the condenser, fully loosen the secunng knob ®, then remove
the condenser by lowenng lt wlth the condenser height adiustment knob
©, and wipe the front lens Of the condenser.
The condenser can be attached by reversing tne above removal
procedure.
6. When dlsposlng of the microscope. Check the regulations and rules
of your local government and be sure to observe them

a
Intended
use
This product has been designed to be used to observe magnified Images of specimens in vanous routine wo.rk and
research applications.
Do not Li§e this product for any Duroose other than its intended use.
Thl§ product is considered a medical device in the following country: uS.
This product complies with the requirements of clirectivo 9979/EC conoeming in vitro diagnc)§tic
( € medical devices. CE marklng means the conformity to the directive
a
Caution
lf the microscope is used in a manner not specified by this manual, the safety of the user may be imperiled.1n addition,
the equipment may also be damaged. Always use the equipmerit as outlined in this Instruction manual
; The following symbols are used to set off text in this Instruction manual.
: A. Indicates that failure to follow the instructions in the waning could result in bodily harm to the
user and/or damage to equipment (including objects in the vicinity of the equipmenD
; * lndlcates that failure to follow the instructions could result in damage to eciuipmem
: ©. Indicates commentary (fcir ease of operation and maintenance).
__----..-_----------------------------------------.--.-----....--------------------------.----.-...'
Safety and EMC Precautions
• This preduct complles with the emission and Immunity requlrements descTibed in lEC61326 series
• The electromagnetic environment should be evaluated prior to operatlon of thls product. Do not use this product in
close proximity to the §c)urces Of strong electromagnetic racliatlon to prevent ln`9rrerence wlln llie proper c]peidllun
• Use only power cord which OLYMPUS speoltleB 0thorwl§e the safety and EMC perfomance of the product can ncit
be assLired.
• Be sure to ground the prodiict Othervi§a oLir intended electric safety and EMC perfomance of the product can not
be Eissured

CX21LED
©Af[er opening the package, make sure that the correct component units for the selected set are present.
• The loox objective and immersion oil are provided only with the CX2l LEDFsl set
*Tne ob|ectlves nave been screwed ln tlglitly to prevent them from belng loosened during transportation. To
remove an ob|ective, turn lt counterclockwise while holding it with a rubber sheet ctc. so that your fingers dom slip.
• Pllter Holder
• Eyeplece§
WHC15X
WHC15X-H
CH2-FH-
I Optlonal AcceesorleB
• DaTkfleld Pllng
CH2-DS
• Peflectlon Mirror
CH20-MM
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@
4

\
©The following items have been` attached at the factory to prevent detenoration during transporfu Pemove these Items and
retain them for future use. Then loosen the observation tube clamping knob and correct the orientation of the eyepieces
as shown in the illustration belc)w
© Bevolvino noseoiece/observation tube transport band
© Stage and specimen holcler protection sheet
© Protective pad below the stage
©Attach the AC adapter and power cord as described in chapter 9, "ASSEMBL/' on page 17
Obgervation tube clamping
knob
©For detailed descnption of each control, refer to the page indicated Inside parentheses.

CX21LED
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Sot the main swltch to " I " (ON) and adjust
the brightness.
(Controls Used)
®Main Switch
®ught intensity aclju§tment knob
(Page)
(F' 7)
(P7)
Place the specimen on the stage.
_________..®Snecimen holder
©X-axisrv-axis feed knobs
(E7)
Engage the lox objoctlve in the light path.
-------------------------©F]evolvingnoseplece
(P 10)
Bring the specimen in focus
------ ©Coarse/fine focus adjustment knobs (P 8)
Adjust the interpupillary dlstance.
Adjust the dlopter,
©Binocular tube
®Diopter adjustment ring
Adjust the aperture lri§ diaphragm.
-------------------------©Apertureir`sdiaphragmring
(P 10)
Engage the obJectlve to bo used in the light
patri and bring the specimen ln focus.
®Plevoiving nosepiece
©Coarse/fine adjustment knobs
(P 10)
(p8)
Engage the requ]red filters.
------- ®PIIters (Diameter 45 mm) (P5)
Adjust the brightness.
------- ©ught lntensfty adjustment knob (F' 7)
Start obgervaton.
6

q
Turning
the LED Illumination ON
(Flg. 3)
1. Set the main switch © to " I " (ON).
2. Botating the light intensity adjustment knob © in the directlc)n of the
arrow increases brightness and rotating it in the opposite direction decreases
brigr`tness. The figures arounc] the knob lnc]lcate the reference
brightness.
a
Placing
Specimen on the Stage
(Plg§. 4 & 5)
* Place the specimen gently. 1{ the bow-shaped lever ls returned with
a strong force or the knob ® of the bow-shaped lever i§ released in
the mlddle, the slide glass may be broken.
1. Potate the coarse adjilstment knob © in the direction of the arrow to fully
lower the stage.
2 Open thEt bow-Shaped lever © outward, place trie specimen by sllcling
the specimen glass plates on the stage from the front toward the rear.
3 After Sliding the specimen glass plates all the way, return the bow-shaped
lever ® gently.
4. Botating the upper knob which ls the Y-axis teed knob © moves the
speclmen in the vertical direction. Botatlng the lower knob which ls the X-
axls feed knob ® moves it in the horizontal dlrectlon.
*Do not move the specimen holder dlrectly by hand, for this win
damage the rotary mechanl§ms of the above kncibs.
*When the specimen holder reaoheo the otoppor poeitlon, the
rotation force of the above I(nobs become heavy. Stop rotating
the knob at thls tlme.

Speclmen holder scales (Fig. 6)
©Theses scales allow the pcisition (coordlnates) being observed on the
specimen to be identified. Even after the specimen is moved, it can be
returned easily to the original position.
1 The horizontal Coordinate can be read at posltlon ® on tne Speclmen
holder
2. The vertical coordinate can be read at the position of index line ©.
a Adjusting
the Fbcus
(Plg. 7)
Focuslng Procedure
1. Plotate the coarse adjustment knob ® in the direction of the arrow so
that the objective © io ae close a3 po33ible to the specimen.
2. While observing the spocimen through the eyepieces, slowly rotate the
cciarse adjustment knob ® in the opposite clirectictn tct the arrow tc> lower
the stage.
3. When c,oarse focusing of the sr)ecimen is obtained, rotate the fine
adjustment knob © tc) ad|ust to precise focus.
working Dlstance WD)
©The WD refers to the distance between each objectlve aiid the sLieciHitjH,
when precise focus of the specimen is obtalned.
i:i|genTfi:eation
4X lox 40X 100X
WD (mm) 18.5 1 l'\t\ 0.6 0.13
Ad|u6tlng the Tenslon of the Coarse Ad|ustment Knob (Plg. 8)
1. The tenslon of the coarse focus adjustment knob has been designed
adjustable with a ring Insert the tlp ctf a large tlat-blade screwdrlver lntrt
the groove © on the tension adjustment ring ® and rotate the ring.
Botating it G!ockwise (in the direction of the arrow) increases the tenslon
and counterclockwise decreases the tension.
2. If the stage descends on its own or if the specimen gets out of focus
qulckly even when 11 ls brought Into focus using the fine adjustment
knob, it means that the tension of the coarse adjustment knob is too low.
Turn the ring ® in the direction of the arrow to increase the tension.

Pre-focusing Knob (Pig. 9)
©The profocusing knob controls the mechanism for preventing collision
between lne speclrrien and objectlve.
1. After bringing the specimen Into focus, turn the pre-focusing knob ®
inside the hole of the arm so that the pre-focuslng mechanism hits the
stage guide
2. To provide a certain margin for focusing, rotate the knob by about half
turn backward from the stopped position.
* lf the function Of this mechanism ls not required, set the pne-focuslng
knob ® at the highest position.
a I Adjusting the lntgrpupillary DIstanc-6
(Plo. 10)
©The interpupillary distance adjustment conslsts of regulating the two eyepieces
according to that between your eyes so that you can observe a
single microscopic image through two eyepieces. This greatly helps to
reduce fatigue during observation
• While looking through the eyepieces, move both eyepieces until the left
and right fields of view coincide completely.
The position of index dot . indicates the interpupillary distance value.
©Note your interpupillary distance so that it can be quickly duplicated
Pig, 10
a Ad|ust!ng the Diopter
(Plo.11)
®The dlopter adjustment consisls of compensating for the clliferenco ln
eyesight between your eyes.
1. Whlle looking through the right eyepiece with your right eye, rcttate
the coarse and fine focus adjustment kncibs to bring the specimen
Into focus.
2 While looking through the left eyepiece witr\ your left eye, rotate only the
diopter adjustment ring © to focus on the specimen.
Uslng the Eye Shades (Pig. 12)
When Wearing Eyeglasses
Use with the eye shades in the normal, foldedrdown position. Ttiis w"
prevent the eyeglasses from being scratched
When Not Wearing Eyeglasses
Extend the folded eye shades in the direction of the arrow to prevent
extraneous light from entenng between the eyepieces and eyes
Pig.12

CX21LED
aa)
Adjusting the Condenser Positl-on
and Aperture Iris Diaphragm (Flg. 13)
©The condenser is usually used in the highest position. If the entire observed
field of view is not bright enough, brightness may bo improved by lowering
the condenser slightly.
1. Potate the condenser height adjustment knob ® to move the condenser
to the highest position.
2, The aperture iris diaphragm ring ® has an objective magnification
scal© (4X, lox, 40X,100X) F`otate the ring so that the magnification
of the objective ln use faces frontward
Pig. 13
Swltching the Objectives
#,`;.I¢ti'.-;€t¥-y-vi (Plg. 14)
Hold and rotate trie revolving nosepiece ® so that the objective to be
used come exactly abcjve the specimen.
Fig. 14
I,_

a Using
the 100X Immersion Objective
(Plg.15)
Plo.15
©The designated immersion oH should be attached to the top lens of the
100X immersion obi.ec`tive Otherwise, the observed image will be unable
to be focused on.
*Always use immersion oil supplled by Olympus.
1. Focus on the speclmen uslng all objectlves, startlng from the lowestpower
objective to higr\er-power objective.
2. Before engaging the immersion objective in the light path, place a drop
of provided immersion oil onto the specimen at the area to be observed.
3. Botate the revolving nosepiece to engage the immersion objective and
rotate the fine adjustment knob to bring the specimen into fc>cus.
*Slnce air bubbles ln the oll will affect the image quality, make sure
that the oil ls free of bubbles.
To remove bubbles, rotate the revolving nosepiece slightly to move
the oll-Immersed objective by one reciprocation or two.
©The c,ondenser of this microscope manifests the full performance when
oil is placed between the slide glass and the front lens of condenser lf oH
is not attached there, the observation image may become slightly dark
4 After use. remove oil from the objective front lens by wiping with gauze
slightly moistened with absolute alcohol.
Acautlon ln use Of lmmerslon all
[f immersion all enters your eyes or contacts your skin, immecliately
take the followlng treatment.
Eyes: F}lnse wlth fresh water (for 15 minutes or more).
Skin : F`ioe with water and soap,
11 the appearance of the eyes or skln ls altered or palm persists,
lmmedlately See your doctor.

How To lTack a Mlcroscoplc Image
Dlrectlon of moving the Image
©The image observed through the microscope moves in
directions opposite to the actual up-down and left-right
movements of the specimen.
Total Magnlflcatlon
The size of the specimen image for observation i§ obtained by multiplying the eyepiece magnlflcation by the
objective magnificatic)n. This value is referred to as total magnificatictn.
Example: Eyepiece (lox) x objective (40X) = 400X
Fle8olutlon
Plesolu[lc)n delerrTilnes how nnely a lens ls able to distinguish the delail5 ctf a sF)ecimeri
The re§olutlon of a mlcrc)scope 1§ malnly determined by the ablllty o[ the objeclive and is scarcely related [o that of the
eyepiece8. The only functic)n of the eyepieces ls to magnlfy an Image already re§ctlved by the cib|ective
Althouoh hath eonflguratlon§ above provide the same total maonlflcation, the higher maonifi[atlon of THE c)bjective
yields a better speclmen reso[utlon
Fleld Number (FN)
The field number ls the dlameter of the image cibserved through an eyepiece, represented in mnlimeters,
10Xeyepiece.18mm
Actual Fleld of Vlow
WHC15X.12mm
£88[#Lha°|i9oTamc'tual
field of vlew.
The actual field of view is the size on the specimen actually
observed in the microscope. This yields approximate size of
the actual specimen.
Actual field of view - Pleld number/Objective magnification
When the lox eyepiece above and lox objective are used,
the actual field of view is equal to:
Actual field of view -18/10 -1.8 mm
Actual field of
view: 1.8 mm

--±iEEEEE-LIRE-nl_F!M..I.rmTmrm\Im:__±`
Under certain conditions, perfomance of the unlt may be adversely affected by factors other than defects, lf problems occur,
please review the following list and take remedial action as needed. If you cannot solve the problem after checking the entire list,
pleaee Contact Olympus for aeeistance.
-`c` ©fflT!FTFay Page
1. Uneven brightness in observation The objective is not engaged in the light Engage the objective into position until
10
field. path. i' clicks.
The condenser is too low. False lt to the upper limit 10
The objectlve, eyepiece, condenser and/orwindowlensaredirty. Clean them thoroughly.
2
2. Dust or stains are visible in obser- The eyepiece, condenser, window lens Clean them thoroughly.
vation field.
and/or specimen glasses are c]irty
2
3 0b§ervatlon Image glares. The conclenser is too low. Raise it 10
The condenser lrls diaphragm ring is Adjust the aperture according to the obstopped
down excessively.
jective mag nification.
4 Observation image is whitish- The ctb)ective ls not engaged in the light Engage the clbjective into positlon until
blurred or unclear path. t cllcks.
The objective, eyepiece, condenser and/orspeGimenglassesaredlrty. Clean them thoroiighly.
10
10
2
Immersion oH io not used with an immer-sic)nobjective,
Use lmmerelon ctll.
11
Bubbles are mixed in the immersion oil. Bemove the bubbles. 11
The specified imm©r§ion oH is not used.
Use the Immersion oil suppliecl byOIympus.
11
5. Part of Image ls defocused or image The objective is not properly engaged in Engage the objective Into posltlon until
loots llke l['s flowing. the 119ht path t cllcks'
The §pecimen i§ not set properiy on the Set the spocimen correctly on the Stage
staoe
ancl secure using the §peclmen holder,
6. High-magnification cibjectlve The sr)eclmen is upside down. Set the specimen correctly with the
touches specimen just beforecomlngIntofocus`
cover glass on the top.
10
7
7
7 llie tension of coarse adjustment The coarse adiustment knob tension ad- Loosen the ring to adjust to proper
knob 1` too high.
tension
)ustm©nt ring ls sEit too tighl.
8 Focu§ing is lmpos§lble (because The pro-focusing knob is positioned toolow F`alse lts position`
the stage cannot be raised).
9 The stage lowers by its own weight The coarse adjustment knob tension Tighten the ring to adjust to proper tenor
focueing i§ lost due to Slippageoftheknob. adjustment ring is set too loose sion.
8
9
8
10.Coarse focus adjustment cannotlowerthestagelowenough. The condenser i§ tot) low Fiaise it.
10
11. Flelds of view of two eyes do not The interpupillary disfance is not adjusted Adjust it properly.
match.
propeny.
Dlopter c;ompensation fcir the two c,ye3 ionotset. Adjust it conectly.
9
9
The left and right eyepieces are different. Beplace one of them so that the leftandrighteyepiecesareidentical. -

13.The LED illumination does not light. The AC adapter or power cord ls ur\-pluoged Plug 1[ securely
17
ERE: `:(,..I.:-`,,.!!`i;LJ;, . - - ,;-, ,. -.;:;'y -,. `-``. .---r---`l=,
§wffEgr#6dyL `' Page
12. Objective hits the speclmen when The speclmen ls upslde clown. Set the specimen correctly with the
an objective is switched to a higher-magnificationobjective.
cover glass on the top.
7
The cover glass is too thick
Use a cover glass with thickness of0.17mm.
7
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Item
I10ptlcalsystemI2.Illumination
§pecmcatlons
Ul§2 (Universal Infinity System) optical systemBuilt-in0.5WLEDilluminationsystem.
Bated voltage: 5€ V 0.5 A ==VACadapter'loo-240 V ru 50/60 Hz 04A)Powerconsumption:1.7W
3. Focusing mechanism
4. PevoMng nosepiece
5. Binocular observation tube
Stage height adjustment mechanism.
Flne ad|ustment scale: 2 5 Llm per graduation
Fine adiustment stroke; 0 3 mm per turn
Total stroke: 20 mm.Pfs.nknobprovided coarse adjustmentknobtenslon adjustablo
re-OCU 10Quadruplepositions fixedFieldnumberTut)etlmngangle
(Fro nt oriented)
18
BOO
lnterpu pi llary distanceadjustmentrange
48 to 75 mm120x132mm (wl`n mecnanlc;al stage)
6. Stage
SizeMovement rangeSpecimenholclerType
76 0(-exis) x 30 rv-axis) mm
Holds a single specimen.Abbecondenser
7 Condenser
N.A.
125 (when Immersed ln oil)
Aperture Iris diaphragm
Built in
6k
8, D`mensions & weight9.Operatingenvironment
154Wx391(H)x238(D) mm,approx
g•lndooruse,
• Altltude: Max. 2000 meters•Ambienttemperaturel5°to 40°C (41 a to |04° F)•Maximumrelatlvehumid.ity80°fufortemperatures up tD 31 ac (88°F),decreaslng\inoarlythrough7C)%at34°C
(936F), 60% at 37°C (99°F), `0 50% relative
humiclfty al 4ooC (1o4oF).
• Supply voltage fluctuatIPo\lilticindegree:2(ln•lnstalIatfon/Overvc]Itagions; Nc)t `o exceed ±10% of the normal voltageaccordancewithlEC60664-i)ecategory:11(inaccordancewlthlEC60664-1)

The following table shows the ciptical characteri§tlcs of combinationsofeyepiecesandobjectives.Thefigureontheright
shows the performance data engraved on the obiectives.
Objective type (on the back slde) ,''
Plan Objeouve
Characte°ripstit#Objectwes
Power
N.A.
W.D.(mm)
CoverGlass
Thlckness
Pesolutlon(LJm)
10XEyepleces (FN 18)
Fbcra'
TotalPower
i;Rt,h
Actual
Ple'd ofVlew
Ftemark
Plan objective (FN 22) 4X OJO 18.5
336 40X 175.0 45
lox 025 10.6 1.34 loox 28.0 18
40X 0.65 0.6 0.17 052 400X 3.04 0.45
100XO 1.25 0.13 0.27 1000X 069 018
Oil Immersed
Worklrig cli8tence twD) . Distance between thet tclp surface or cover glass and the ob|ec[lve extremlty.
Number of aperture (NA) . The ficiure corresponcling lo the F-number of the camera Thls ls associated with the resolutlcin
and larger NA means higher resolutictn
Besolution :
Fcical depth :
(Obi.ect 9idc) :
Field number GN) :
Actual Held of view ,
Total F)ower .
ITotal mag nific;ation)
Ablllly of an objectlve for ldentifying adiacent two lines in the Image, which is expressed in terms
of the mlnlmum distance between two points on the specimen surface
ThEt clepth range of a speclmen, ln whlch focusing is obtainecl at a time. Stopping Clown the
aperture lris dlaphragm Increases the focal clepth and increasing the obieclive NA decreases it
The diameter of the image observed through an eyeplece, represented in millimeters.
Diameter of the fleld of vlew. expressed as the size on the specimen surface
Ob|ectlve magnification x Eyepiece magnification

© Each standard set can be assembled by simply attaching the AC adapter and power cord.
Connecting the AC Adapter and
Power Cord
(Flg§. 16 & 17)
+Be sure to Llse the provlded AC adaoter Use of other AC adapter
may cause a malfunction.
AThe power cord and AC adapter cord are vulnerable when bent or
twisted. Never subject it to excessive force.
AMake sure that the main switch ® is set to " 0 " (OFF) before cor`nectlng
the power cord.
AAlways use the power cord provided by Olympus. If no power cord
1o provided, please 8eloct the proper power cord by roforrlng to the
section "PPIOPEPI SELEcmoN OF "E POWEP SUPPLY COF`D" at
the end of thls Instruction manual.
1. Connect the output connector ® of the AC adapter to the input connector
© on the rear of tr`e microscope.
ADo not tllt the microscope to the rear. Doing so could damage the
output connector ©.
2 Connect the power coid's connector © [o conneclor ® nrrT\ly.
A Be sure to supply power from a grounded, 3-conductor power outlet
uslng the proper power cord. If the power outlet iB not grounded
proporly, Olympus can no longer warrant the electrical safety performance
clf the t3qu:pment.
3 Connect the power cord's plug © to a wall power outlet ©. frig. 17)
F,g 17

difiREhictesst6iff§
*Can be inserted in the
right side of the standard
lox eyepiece.
Darkfleld Pllng
CH2-DS
45 mm filter
io-2 rirsirin; arid-cteREn
Of opfronal Ajccessorles
q
Fllter Holder CH2-FH and Darkfleld Rlng CH2-DS
(Flg. 18)
-:..i--`-i-:---
•.
,:..i.`
©This holder aGGommoc]ates the CH2-DS darkfleld ring
1 Insert the darkfield nng © in the CH2-FH filter holder ©.
2. Push the filter holder ® containing the darkfield ring ® into the bottom of
the condenser until it clicks into place.
©The darkfield nng enables darkfield obselvation using an objective from
4X to 40X.
Pig, 18

a
Peflection
Min.or CH20-MM eeriL..a (Flg. 19)
©The reflection mirror is designed to be used in microscopic observation in a location where power supply is not avaHable
lt makes it possible to use the natural light in place of the LED lllumlnatlon.
F,g. 19
*The mlcroscope should be ln§tal[ed near a window, in a place that
ls not exposed to direct sunlight.
The eyspleces should be oriented toward ttie rear so that the reflectlon
mirror reeelves the brlght llght. Loosen the observation tube
clamplng knob and rotate the tube by 18oo.
1. Flt the reflection mlrror in the window lens of the microscope frame by
aligning the mounting tab.
2. The reflection mirror ® should be pointed toward the bright area While
ob§enjing the image through eyepieces. adjust the orientatlon of the
reflectlon mirror.
©A planar reflection mirror is used normally. However if the Image brightness
is uneven cirthe outsicle view is visible in the image, use a concave
reflection mirror.
a Eyepleces
WHC15X/WHC15X-H
(Pig. 20)
©The standard lox eyepieces are clamped uslng screws.
1 Using a small flat-blade screwdriver, loosen the clamping screw © of a
lox eyepiece and remove lt.
2. Insert lt`e WHC15X or WHC15X+I into the cyepieco sloove and tighten
the clamping screw ®
Pig. 20

CX2lLED
a( Using the eyeplece micrometer±^`ap``,+` (Plg. 21)
®The eyepiece micrometer can be inserted in a standard lox eyepiece
as well as the WHC15X and WHC15X-H.
However, unlEss the WHC15X-H is used, helic`old adjustment cannot bo
performed, so those with poor eyeslght wlll have trouble ln bringing the
micrometer into focus.
• Get a micrometer with diameter of 19 mm and th`ckness of 1 mm.
• Bemove the micrometer sleeve © and fit the micrometer ® into the
eyepiece with the indication side facing down as shown in Flg. 21.
Pleplace the mlcrometer sleeve to use the micrometer.
20

I PROPER SELECTloN OF "E POWER SUppl=Y CORD
Ifnopowersupplycordisprovlded,pleaseselecttheproperpowersupplycordfortheequlpmentbyrefernngto"Speclfications"and
" Certified Cord " below
CAutloN:lnoa8oyouu8eanomapprovedpowersupplycordforOlympusproduot§,OIympuscannolongerwarramthe
electrical safety Of the equlpment.
Voltage Paling
Current Bating
Temperature Bating
Lenoth
Pltti ngs C,onfig u ration
125V AC (for 100-120V AC area) or, 250V AC (for 220-240V AC areal
6A minlmum
6ooC minimum
3.05 in maximum
Grounding type attachment plug cap. Opposlte terminates in molded-on lEC configuration
appliance coupllng.
Table 1 Certified Cord
ApowersupplycordshouldbecertifiedbyoneoftheagenciesIIstedlnTablei,orcomprlsedofcordagemarkedwithan
ageney marking per Table 1 or marked per Table 2 The flttlngs are to be matt(ed wi`h at least one Of agencles listed in
Table 1 ln case you are unable to buy locally ln your country the power sllpply cord whlch ls approved by one of the
agencies mentioned in Table 1, please use replacements approved by any othEtr equivalent and authorlzed agencles in
yoiir country
Certlfloatlon
Cortlfl¢atlon
Country Ageney
Country Agency
Man(
Mark
Argentina lFIN ®
Italy lMQ
©
dy,vy
JET, JQA, Tow,uL-APEX/MITl
Australia SAA Japan
V
Austria OVE
Netherlands KEMA
® E
Belgium CEBEC
i?
Norway NEMKO
®
Canada CSA
©.
Spain AEE
©
Denmark DEMKO
®
Sweden sEMrao
®
Plnland FEl
®
Switzerland SEV
®
UnitedKingdom ASIABSI
France UTE
®
®,ty
Germany VDE U.SA UL
ti ®
¢
Ireland NSAI

CX21LED
Table 2 HAFZ Flexible Corcl
APPPOVAL OBGANIZATIONS AND COBDAGE HAF"ONIZATION MAPKING METHODS
Approval Organizatlon
Printed or Embossed Harmonlza-tlonMarking,(#,ay::,i:::I:fwlorn-I
£;;:i:£:,dv.:e,%##n#:k:,gn#ofcolorsectlonlnmm)
i::retorn8ua,On Black Fled Yellow
Comite Electrotechnlque Belge CEBEC (HAH) 10 30 10
(CEBEC)Verband Deutscher Elektrotechniker
IVDE) e.V. PrtlfstelleUnionTechniquede l'Electricite'
(UTE)
lnstitLito ltaliano del Marchlo di
Qualita' (IMQ)
British Approvals Service for Electnc
Cables (BASEC)
(VDE) (HAH, 30 10 10
USE tHAB> 30 10 30
lEM MEQU <HAP> 10 30 50
BASEC (HAH) 10 10 30
N.V. KEMA KEMA-KEUB {HAB> 10 30 30
SEMKO AB Svenska Elektrlska
Materielkc>ntrollanstalter
Osterreichlscher Verband fl]r
Elektrotech nik (OVE)
Danmarks Elektriske Materialkontroll
(DEMKio)
SEMKO <HAPl) 10 10 50
tc)VE> tRAB) 30 10 50
<DEM KO> <HAF) 30 10 30
Natictnal Standards Authority of Ireland (NSAl} (HAH) 30 30 50
(NSA')
Norges Elektriske Materiellkontroll N EM KO (HAH) 10 10 70
(NEMKO)
Asoclacion Electroteonica Y
Electronica Espanola IAEE)
Hellenic Organization for
Stand aid ization (EroT)
lnetituto Flortages da Qualidade
(IPQ)
Schweizerischer Elektro
Technischer Verein (SEV)
{U NED) (HAF}> 30 10 70
ELOT tHAF`) 30 30 70
n p (HAB, 10 10 90
SEV (HAFi} 10 30 90
Elektriska lnspektoratet SETI (HAH) 10 30 90
Undervriters LaDoratones lnc. (UL)
Canadian standards Association (CSA)
SV, S\/T, SJ or SJT, 3 X 18AWG
SV, SVT, SJ or SJT, 3 X IRAWG
-=i=T=±L-I-

OuYMPUS®
The microscope ls a precision instrument. Handle it with care and avoid subjecting lt to a sudden or severe
Impact.
Taking out the microscope
©::e:ayrt°faa:::hwenT|C,|°es:e°ftp:,:t:`re°fotvheer:haec:::set,cb:X:vhe:'fihe
'u::,:hptu:'o°t:ta::hit:C:':ytt:r°mmstuhrfea:eacfaagceo=::nt;Stehtethbeottm::robs:f:Pee
installing.
®.e::e::tti:S;ao'L:h:smu!:;°csec@eWithOtherpartsatthebottoin
• When removing the plastic cover a from the microscope, be
careful not to topple the microscope.
Bemoving protective materials
© ln the condltlon after the CX23 mlcroscope ls taken out of the package, the microscope is provided with various protectlve
matenals for preventing quality degradation dunng transport. Pemove the protective matenals shown in the following
illustration immediately after unpacking.
Ftemove the transport band.
Loosen the observation tube c,lamping
knob slightly, rotate the tube by 180°
so that the observation tube faces the
front direction, and tighten the knob
Peel off the stage protective sheet.
© Bemove all fixing tapes attached tc)
operation parts.
© The package contalns the .Instruction
manual and AC adapter in addition to
the CX23 microscope.
Botate the coarse adjustment knob
slightly in the direction of the arrow to
remove pad.
Pad
Holding the mlcroscope correctly when carrying
© When canying this microscope, hold both sides of the arm parts
`E
as shown ln the picture on the left and carry the microscope
carefully lt is easier to hold the arm hole by puttlng the ball of your
fingers al the finger contact area ill shown in the picture on the left
•DonotholdanypartotherthanarmpartsE}Themlcroscope
may be damaged. Be Sure to remove the §pecimen sllde,
Since lt ls ln danger of falling.
• Do not move the microscope by dragging. The rubber legs
may be damaged or come off dependlng on the table surface
condition.
AX8493 01
OILYI\/lpus CORPORATION