Rudolf Bichele - Archimedes
Rudolf Bichele - Archimedes
Rudolf Bichele - Archimedes
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qPCR<br />
• Lisaks tavalisele<br />
PCRile kasutasime<br />
qPCR (quantitative e.<br />
real time PCR)<br />
• Erinevalt tavalisest<br />
PCRist, kus<br />
fluorestseeruv signaal,<br />
mida annab SYBR®<br />
green, detekteeritakse<br />
pärast igat PCR-i<br />
tsüklit (erinevalt<br />
tavalisest PCRist ja<br />
foreesist, kus signaal<br />
saadakse ainult PCR-i<br />
lõpus).
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