Cortisol ELISA - IBL international

Cortisol ELISA (RE52611)
DEUTSCH
Präzision
Intra-Assay
Inter-Assay
Linearität
Wiederfindung
Saliva (n = 20) Serum (n = 20)
Konz. SD
VK Konz. SD
VK
(µg/dL) (µg/dL) (%) (µg/dL) (µg/dL) (%)
0.27 0.019 7.3 1.8 0.174 9.9
1.48 0.063 4.2 11.5 0.766 6.7
2.34 0.073 3.1 26.2 1.554 5.9
0.54 0.047 8.8 1.5 0.305 20
1.29 0.120 9.3 11.6 2.006 18
2.35 0.151 6.4 20.3 2.712 13
Saliva
Serum
Verdünnung Gemess. Wfdg. Verdünnung Gemess. Wfdg.
(µg/dL) (%)
(µg/dL) (%)
- 0.67 100 1:50 59.2 100
1:2 0.29 85 1:100 30.8 104
1:4 0.16 93 1:200 16.1 109
1:8 0.09 104 1:400 8.6 116
1:16 0.04 93 1:800 4.3 116
1:32 0.02 115 1:1600 1.7 91
- 0.33 100 1:50 59.3 100
1:2 0.17 104 1:100 29.4 99
1:4 0.08 99 1:200 16.4 111
1:8 0.04 98 1:400 7.5 101
1:16 0.02 107 1:800 4.3 117
1:32 0.01 117 1:50 1.7 94
- 1.65 100 1:100 70.9 100
1:2 0.83 100 1:200 35.7 101
1:4 0.41 102 1:400 18.5 104
1:8 0.21 113 1:800 10.2 115
1:16 0.10 107 1:1600 5.0 113
1:32 0.05 109 1:3200 2.2 99
Konz.
(µg/dL)
Saliva 1
(0.13)
Saliva 2
(0.16)
Saliva 3
(0.15)
Saliva
Serum
Zugefügt Gemess. Wfdg. Konz. Zugefügt Gemess. Wfdg.
(µg/dL) (µg/dL) (%) (µg/dL) (µg/dL) (µg/dL) (%)
- 0.13 100 - 10.9 100
0.02 0.16 106 1.5 11.8 95
0.06 0.17 87 Serum 1 3.1 13.8 99
0.20 0.28 86 (10.9) 10.2 19.4 92
0.60 0.61 83 30.6 38.4 92
1.50 1.37 83
76.5 79.9 91
- 0.16 100 - 14.0 100
0.02 0.17 97 1.3 14.3 94
0.06 0.20 92 2.6 14.9 90
Serum 2
0.20 0.30 84 8.7 20.5 90
(14.0)
0.60 0.66 87 26.0 33.8 85
1.50 1.53 92 65.0 77.3 98
173.4 191.2 102
- 0.15 100 - 19.5 100
0.02 0.16 91 1.5 21.4 102
0.06 0.22 102 Serum 3 3.1 23.9 106
0.20 0.33 94 (19.5) 10.2 28.6 96
0.60 0.74 98 30.6 49.9 100
1.50 1.54 88
76.5 93.0 97
Saliva IBL-ELISA = 1.09 x IBL-Luminszenz IA + 0.01 r = 0.996; n = 82
Methodenvergleich
Serum
IBL-ELISA = 1.06 x IBL-Luminszenz IA - 1.44 r = 0.987; n = 60
IBL-ELISA = 0.84 x GCMS + 0.36 r = 0.918; n = 33
V2009_09 7 / 8