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CLONING 8 Quick Blunting Kit For efficient blunt-end ligations Optimize your blunt-end cloning reactions with the Quick Blunting Kit from NEB. This kit provides a fast and convenient method for preparing sheared, nebulized or restriction enzyme-digested DNA for blunt-ended ligation into a plasmid, cosmid, fosmid or BAC vector. In addition, it can also be used to prepare PCR products generated using non-phosphorylated primers for efficient blunt-end cloning. Advantages of Quick Blunting Kit ❚ Fast – Restriction enzyme-digested DNA blunted in less than 30 minutes ❚ Convenient – Reactions are performed at room temperature in a ready-to-use mix ❚ Flexible – Suitable for restriction enzymedigested DNA, sheared or nebulized DNA and PCR products Ordering Information: Quick Blunting Kit plus FREE Timer #E1201S 20 Reactions £60 #E1201L 100 Reactions £240 Quick Blunting & Quick Ligation Kit #E0542S 20 Blunting Reactions + 30 Ligation Reactions Restriction Enzyme- Digested DNA 5´ 5´ 5´ 5´ 5´ 5´ 5´ A 5´ A 5´ 5´ 5´P OH Blunted Dephosphorylated Vector or Purified PCR or Product Quick Blunting OH P5´ Ligation Transform + Plate Sheared DNA The Quick Blunting Kit facilitates blunt-end cloning reactions. FREE Timer with either of these kits! Available until 30th June 2008. All the advantages of the Quick Blunting Kit PLUS ligations in just 5 minutes at room temperature This bundle of complementary kits comes in two sizes providing enough reagents for 20 or 100 reactions and and is more economical than buying two kits separately. Advantages of Quick Ligation ❚ Fast – 5 minutes for cohesive or blunt ends ❚ Convenient – ligation performed at room temperature ❚ Flexible – suitable for all common ligation reactions Ordering Information: Quick Blunting & Quick Ligation Kit plus FREE Timer #E0542L 100 Blunting Reactions + 150 Ligation Reactions The Quick Ligation Kit may also be purchased separately: #M2200S, 30 Reactions, £80; #M2200L, 150 Reactions, £320 £120 Save £20 compared to buying kits separately £480 Save £60 compared to buying kits separately Using the Quick Ligation Kit protocol, blunt and cohesive inserts were ligated into Litmus 28 vector cut with either EcoR V (blunt) or Hind III (cohesive). Ligation products were transformed into chemically competent E. coli DH-5acells.
SPECIAL OFFER CST Lysis Buffers, Markers and Support Reagents For consistently great results choose the same reagents that CST use for Quality Control testing Lysis Buffers Normal Price Cell Lysis Buffer (10X) #9803 15 ml £36 £25.20 ü for lysis of adherent and non-adherent cells – simply add 1 mM PMSF immediately before use ü 10x buffer can be used for lysis of tissue samples (with homogenization) ü used to lyse cells under nondenaturing conditions ü 10x buffer can be kept at 4°C for 1-2 weeks (or at -20°C for longer periods) ü additional protease inhibitors can be added 1X concentration: 20 mM Tris-HCl (pH 7.5) 150 mM NaCl 1 mM Na 2 EDTA 1 mM EGTA 1% Triton 2.5 mM sodium pyrophosphate 1 mM beta-glycerophosphate 1 mM Na 3 VO 4 1 mg/ml leupeptin RIPA Buffer (10X) #9806 15 ml £36 £25.20 ü for lysis of adherent and non-adherent cells – simply add 1 mM PMSF immediately before use ü 10x buffer can be used for lysis of tissue samples (with homogenization) ü used to lyse cells under nondenaturing conditions ü 10x buffer can be kept at 4°C for 1-2 weeks (or at -20°C for longer periods) ü additional protease inhibitors can be added 1X concentration: 20 mM Tris-HCl (pH 7.5) 150 mM NaCl 1 mM Na 2 EDTA 1 mM EGTA 1% NP-40 1% sodium deoxycholate 2.5 mM sodium pyrophosphate 1 mM beta-glycerophosphate 1 mM Na 3 VO 4 1 mg/ml leupeptin 30% OFF * RIPA Buffer (10X) #9806 15 ml £36 £25.20 Cell Lysis Buffer (10X) #9803 15 ml £36 £25.20 CHAPS Cell Extract Buffer (10X) #9852 5 ml £36 £25.20 Protein Markers Biotinylated Protein Ladder Detection Pack #7727S #7727L Prestained Protein Marker, Broad Range (Premixed Format) #7720S #7720L Loading Buffers 65 mini-blots 325 mini-blots 70 lanes 350 lanes Blue Loading Buffer Pack #7722 8 ml £14 £9.80 Red Loading Buffer Pack #7723 8 ml £14 £9.80 General Support Reagents CST Cell Lysis Buffers – validated with CST antibodies to guarantee reproducible results £36 £144 £62 £245 Offer Price £25.20 £100.80 £43.40 £171.50 BSA #9998 50 grams £144 £100.80 Nonfat Dry Milk #9999 250 grams £36 £25.20 Phosphate Buffered Saline (PBS-20X) #9808 1000 ml £54 £37.80 Phosphate Buffered Saline with Tween 20 (PBST-20X) #9809 1000 ml £62 £43.40 Tris Buffered Saline with Tween 20 (TBST - 10X) #9997 1000 ml £36 £25.20 ELISA Reagents STOP Solution #7002S #7002L TMB Substrate #7004S #7004L Kinase Assay Reagents 100 ml (10 x 96 well plates) 500 ml (50 x 96 well plates) 100 ml (10 x 96 well plates) 500 ml (50 x 96 well plates) £20 £70 £36 £144 £14 £49 £25.20 £100.80 ATP 10mM #9804 1 ml (100 Assays, 96 Well Format)) £36 £25.20 Kinase Buffer (10X) #9802 15 ml £36 £25.20 * 30% discount is available to UK customers on the products listed above until 30th April 2008. No other discounts apply. Chaps Cell Extract Buffer (10X) #9852 5 ml £36 £25.20 ü for lysis of cells under denaturing conditions – simply add 5 mM DTT (200X conc. included) and 1 mM PMSF immediately before use ü can be used under nondenaturing conditions ü for the preparation of cytoplasmic cell lysates to be used with CST Caspase signaling pathway antibodies 1X concentration: 50 mM Pipes/HCI (pH 6.5) 2 mM EDTA 0.1% Chaps 20 mg/ml Leupeptin 10 mg/ml Pepstatin A 10 mg/ml Aprotinin For use with Caspase Antibodies Cell Signaling Technology News 9
Page 1 and 2: NEB UK Update Spring 2008 NEW ENGLA
Page 3 and 4: ColorPlus Prestained Protein Marker
Page 5 and 6: High Fidelity (HF) Restriction Enzy
Page 7: High Performance PCR THE INTEGRATED
Page 11 and 12: Light chain 3 (LC3), which can serv

NEB UK - New England Biolabs (UK)

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