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Stool sample processing

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Selective media commonly used to recover<br />

diarrheal agents<br />

CULTURE<br />

MEDIA<br />

Thiosulphate<br />

bile salt<br />

sucrose agar<br />

(TCBS)<br />

Cycloserine<br />

cefoxitin<br />

fructose agar<br />

(CCFA)<br />

Sorbitol<br />

macconkey<br />

agar (SMAC)<br />

Purpose<br />

Vibrio species<br />

Aeromonas species<br />

selective media for<br />

clostridium difficle<br />

A differntial media to<br />

detect sorbitol<br />

negative E.coli<br />

(contain sorbitol<br />

instead of lactose)<br />

Pathogens<br />

Yellow colonies; sucrose fementing vibrio sp.<br />

Such as V. cholera<br />

Blue green colonies: non sucrose fermenters<br />

as V. vulnificus, parahaemolyticus<br />

Appeasrs yellow from fructose fermentation<br />

E.coli O157: H7 apear colorless

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