Stool sample processing
Stool sample processing
Stool sample processing
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Selective media commonly used to recover<br />
diarrheal agents<br />
CULTURE<br />
MEDIA<br />
Thiosulphate<br />
bile salt<br />
sucrose agar<br />
(TCBS)<br />
Cycloserine<br />
cefoxitin<br />
fructose agar<br />
(CCFA)<br />
Sorbitol<br />
macconkey<br />
agar (SMAC)<br />
Purpose<br />
Vibrio species<br />
Aeromonas species<br />
selective media for<br />
clostridium difficle<br />
A differntial media to<br />
detect sorbitol<br />
negative E.coli<br />
(contain sorbitol<br />
instead of lactose)<br />
Pathogens<br />
Yellow colonies; sucrose fementing vibrio sp.<br />
Such as V. cholera<br />
Blue green colonies: non sucrose fermenters<br />
as V. vulnificus, parahaemolyticus<br />
Appeasrs yellow from fructose fermentation<br />
E.coli O157: H7 apear colorless