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Efficacy of new-generation acaricides in controlling mites in apple ...

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348 Alicja Maciesiak and Ryszard W. Olszak<br />

MATERIAL AND METHODS<br />

The experiments were conducted <strong>in</strong> 2001–2004 <strong>in</strong> several commercial orchards<br />

<strong>in</strong> some regions <strong>of</strong> Poland. The effectiveness <strong>of</strong> the <strong>new</strong> <strong>acaricides</strong> Envidor 240 SC<br />

(spirodicl<strong>of</strong>en) and Milbeknock EC (mibemect<strong>in</strong>) was compared with the standard<br />

preparations Mitac 200 EC (amitraz), Omite 30 WP (propargite), Ortus 05 SC (fenpyroximate).<br />

The experiments, conducted <strong>in</strong> 4 replicates, were set up <strong>in</strong> a block design<br />

with plots <strong>of</strong> 0.1–0.3 ha each as a comb<strong>in</strong>ation. Treatments were performed with a<br />

tractor sprayer us<strong>in</strong>g 600–750 l <strong>of</strong> spray<strong>in</strong>g liquid per ha. Mobile forms <strong>of</strong> spider<br />

<strong>mites</strong> were counted on leaves sampled from the external part <strong>of</strong> the crown <strong>of</strong> trees<br />

grow<strong>in</strong>g <strong>in</strong> the middle part <strong>of</strong> the plot. Population density was evaluated with HEN-<br />

DERSON & MCBURNIE’s (1943) method on 50 leaves for each replicate and date. A<br />

n=<br />

k−1<br />

t n<br />

cumulative <strong>in</strong>dex <strong>of</strong> <strong>in</strong>festation (CII = ∑ ( ⋅(<br />

x ))<br />

= 1<br />

n + x<br />

n<br />

n+<br />

1 mite unit days where k<br />

2<br />

is the number <strong>of</strong> occasions on which the <strong>mites</strong> were counted; n , n+<br />

1 are consecutive<br />

<strong>in</strong>dices; is the time, <strong>in</strong> days, between the consecutive <strong>in</strong>dices )<br />

(WRATTEN et al. 1979) was calculated for each test. The results were subjected to an<br />

analysis <strong>of</strong> variance. Duncan’s t test was employed to evaluate the significance <strong>of</strong><br />

differences at P=0.05.<br />

x x<br />

t n<br />

n , n+<br />

1 x x<br />

2<br />

Table 1. Effectiveness <strong>of</strong> Envidor 240 SC and Milbeknock EC <strong>in</strong> controll<strong>in</strong>g <strong>mites</strong> on <strong>apple</strong> trees (2001–<br />

2002)<br />

Treatment<br />

Rate/ha<br />

Mean no. <strong>of</strong> <strong>mites</strong> per leaf<br />

Weeks after treatment<br />

3–4 5–6 7–8 11–12<br />

Location A – Grzymkowice, near Grójec, spray<strong>in</strong>g date 17 May 2001<br />

Envidor 240 SC - 0.4 l 0.0 a* 0.0 a 0.0 a 0.1 a 0.5 a<br />

Milbeknock EC - 1.0 l 0.0 a 0.0 a 0.1 a 0.0 a 1.7 ab<br />

Omite 30 WP - 2.25 kg 0.3 b 0.02 a 0.05 a 0.5 a 5.1 b<br />

Control 1.9 c 4.7 b 3.1 b 3.3 b 100.0 c<br />

Location B – Wilcze Piêtki, near Grójec, spray<strong>in</strong>g date 25 May 2001<br />

Envidor 240 SC - 0.4 l 0.0 a 0.0 a 0.0 a 0.1 a 0.6 a<br />

Milbeknock EC - 1.0 l 0.01 a 0.0 a 0.0 a 0.1 a 1.0 a<br />

Omite 30 WP - 2.25 kg 0.5 b 0.0 a 0.8 ab 1.3 b 26.6 b<br />

Control 0.4 b 1.1 b 3.0 c 3.6 c 100.0 c<br />

Location C – Muchnice, near Kutno, spray<strong>in</strong>g date 10 May 2002<br />

Milbeknock EC - 0.75 l 0.6 b 0.4 b 0.3 a – 4.5 a<br />

Milbeknock EC - 1.0 l 0.1 a 0.02 a 0.1 a – 1.2 a<br />

Ortus 05 SC - 1.5 0.1 a 0.7 b 2.4 b – 8.6 a<br />

Control 18.8 c 10.8 c 12.1 c – 100.0 b<br />

Location D – Nowa Wieœ, near Grójec, spray<strong>in</strong>g date 9 May 2002<br />

Milbeknock EC - 0.75 l 0.03 a 0.0 a 0.02 a – 0.8 a<br />

Milbeknock EC - 1.0 l 0.05 a 0.0 a 0.0 a – 0.5 a<br />

Ortus 05 SC - 1.5 l 0.0 a 0.0 a 0.0 a – 0.0 a<br />

Control 8.7 b 1.7 b 3.8 b – 100.0 b<br />

CII – cumulative <strong>in</strong>dex <strong>of</strong> <strong>in</strong>festation (control = 100%)<br />

* Means followed by the same letter with<strong>in</strong> columns do not differ significantly at P=0.05<br />

CII<br />

(%)

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