SepMate™ Hassle-Free PBMCs in Just 15 Minutes - Stemcell ...

Isolate PBMCs from Whole Blood in Just 15 Minutes with SepMate TM
SepMate TM is the fast, easy and foolproof way to carry out density gradient centrifugation. The SepMate TM insert eliminates the need for
painstakingly slow density gradient layering and PBMC harvesting, allowing you to obtain purifi ed, viable and functional mononuclear cells
in just 15 minutes.
Add density
gradient medium
(e.g. Ficoll-Paque TM )
through SepMateTM insert
Ficoll-Paque TM
Layer sample onto
SepMate TM insert
SepMate TM insert SepMate TM insert
Combine SepMate TM and RosetteSep TM to
Isolate Specific Cell Types in One Easy Step.
To isolate purifi ed cell subsets from whole blood, simply
incubate your sample with the appropriate RosetteSep TM
cocktail for 10 minutes before SepMate TM separation.
During centrifugation unwanted leukocytes pellet along
with RBC, leaving untouched and highly purifi ed target
cells in the supernatant.
No columns or magnets are necessary. Just incubate,
spin, and pour off purifi ed cells.
4
5
Centrifuge (Brake on)
15 Minutes
Human CD4 +
T Cell Isolation using SepMateTM-50 and RosetteSepTM Human CD4 +
T Cell Enrichment Cocktail.
Start: 37% CD4 + Cells Enriched: 96% CD4 + Cells
Copyright © 2012 by STEMCELL Technologies Inc. All rights reserved including graphics and images. STEMCELL Technologies & Design, STEMCELL Shield Design, Scientists
Helping Scientists, RosetteSep and SepMate are trademarks of STEMCELL Technologies Inc. Ficoll TM and Ficoll-Paque TM are trademarks of GE Healthcare Ltd. Lymphoprep TM
is a trademark of Nycomed.
Counts
Hassle-Free PBMCs in Just 15 Minutes
Plasma
PBMCs
Ficoll-Paque TM
RBCs and granulocytes
Isolate Highly Purifi ed Cell Subsets Directly from Whole Blood
in 25 Minutes
For more information on RosetteSep TM cocktails, please visit www.rosettesep.com.
Counts
Pour
CD4 APC CD4 APC
PBMCs