J_Environmetal Microbiology and Engineering

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J-28 Characterization of Microbial Community of the Ammonia Oxidizing Enrichment Culture from Wastewater Treatment Plants Hyoun Duk JUNG and Eun Young LEE* Department of Environmental Engineerin and Environmental Microbiology, Suwon University, Gyeonggi 445-743, Korea. *Corresponding author: ley@suwon.ac.kr Ammonia oxidizing enrichment culture carried from S wastewater treatment plant was performed in the AOB medium. The microbial community in wastewater treatment plants were characterized using potential carbon availability method. For the Ecoplate (Biolog, USA) analysis, those samples were inoculated and cultured at 20℃. Color changes of individual well in the Ecoplate according to cell growth were measured at 595nm and average well color development (AWCD) was calculated. The order of AWCD values of S1(enrichment culture), S2(aeration tank), S3(effluent)and S4(influent) were 0.36,1.18,1.12 and 0.98, respectively.The Shannon indies were 2.31(S1), 3.35 (S2),3.37(S3) and 3.38(S4),respectively. In addition, Bacterial community was characterized using 16S rDNA PCR and denaturing gradient gel electrophoresis (DGGE) fingerprinting method. the similarities of DGGE fingerprints were 7% between S1 and S2, 11% between S1 and S3, 7% between S1 and S4, 11% between S2 and S3, 8% between S2 and S4, 13% between S3 and S4 of the ammonia oxidizing enrichment culture from wastewater treatment plants. Keywords: DGGE, Ecoplate, wastewater Reduction of Tellurite and Formation of Tellurium Nanostructures by Shewanella oneidensis MR-1 Dong-Hun KIM 1 and Hor-Gil HUR* 1,2 J-29 1 2 School of Environmental Science and Engineering. International Environmental Analysis and Educational Center, Gwangju Institute of Science and Technology, Gwangju 500-712, Republic of Korea. *Corresponding author: donghun@gist.ac.kr The metalloid oxyanion tellurite(TeO3 2- ) is highly toxic to most organisms and specific mechanisms explaining its toxicity are not well known to date. In this study we determine the reduction of tellurite and formation of tellurium by S. oneidensis MR-1. Strain MR-1 can reduce tellurite [TeO3 2- ] to elemental tellurium [Te(0)] under anaerobic conditions. This reduction result in the formation of unique crystalline Te nanoarchitectures as end products. The localization of needle-like shaped Te nanostructures are controlled by culture conditions. EDX and SAED indicate that nanostructures are composed entirely of Te and are crystalline. Furthermore, c-type cytochrome deletion mutants and random mutants from strain MR-1 shown lower reduction rate of tellurite than wild type and different morphologies on tellurite contained solid medium. Additional works needs for describe more details about molecular mechanisms of the tellurite reduction and formation of nanostructures. This research offers the potential to combine remediation of contaminants with the development of environmental friendly manufacturing pathways for novel biogenic nanominerals and recovery of rare metals from industrial waste water. Keywords: Keywords: Shewanella, Tellurium, dissimilatory metal-reducing bacteria (DMRB), nano structure 2011 International Symposium & Annual Meeting J-30 Improved Performance of a Microbial Electrolysis cell using a Membrane Electrode Assembly Cathode Yuhong JIA, Jae Hun RYU, Cho Hui KIM, Woo Kyung LEE, Thi Van Trinh TRAN, Hyo Lee LEE and Dae Hee AHN* Department of Environmental Engineering and Biotechnology, Myongji University, Yongin 449-728, Korea. *Corresponding author: dhahn@mju.ac.kr Microbial electrolysis cell is a device which can produce hydrogen gas from biomass through microbial catalyzed process and thus reduce the organic matter. For the real application in wastewater treatment, the scale-up of microbial electrolysis cell is an important issue but few tests were conducted with relatively large size. In this study, a 3.7 L microbial electrolysis cell equipped with a membrane electrode assembly cathode was designed and tested. The internal resistance was examined, hydrogen generation and organic removal performance was investigated under different conditions. An internal resistance of 7 Ω was examined from the reactor with a relatively large working volume. The MEC achieved a coulombic and cathodic efficiency of 55 % and 74 % respectively at an applied voltage of 1.2 V, results a maximum overall hydrogen efficiency of 41 %. This corresponding to a volumetric hydrogen production rate of 0.12 L-H2/LA-day (A = Anodic liquid volume) at a hydrogen yield of 1.64 mol H2/mol acetate and a current density of 2.8 A/m 2 . The hydrogen performance at voltages below 0.8 V was limited, which might be due to the high potential losses in large system. The results obtained in this study could help to further develop pilot-MEC for practical applications. Keywords: Microbial electrolysis cell, Hydrogen production, Membrane electrode assembly Anti-bacteria and Anti-fungal Effects of Herbal Oil made from Halvateria acuminata. Soo Ji KIM* 1 , L.M Udaya CABRAL 2 , Jin Young HONG 1 , Chang Wook JO 1 , Mi Hwa JUNG 1 , Young Hee KIM 1 and Jung Eun CHOI 1 1 Conservation Science Division, National Research Institute of Hultural Heritage, 82 Munji-ro, Yuseong-gu, Daejeon, 305-380, Korea. 2 J-31 Conservation and Preservation Division, National Library and Documentation Services Board, No.14, Independence Avenue, Colombo-7, Sri Lanka. *Corresponding author: feeelsj@naver.com An Ola leaf manuscript, which consists of words carved on an Ola leaf and filled up with mixture of herbal oil made from Halvateria acuminata and charcoal, is one of the native writing media in Sri Lanka. According to historical records, Ola leaf manuscripts had been used from 1 st century A.D to 18 th century A.D. From recent findings that Ola leaf manuscripts have been preserved well for the past 400years and are in good state of preservation, it is supposed that this oil preserves Ola leaves against environment factors and biological factors such as fungi and insects. To evaluate the anti-biological susceptibility of the herbal oil, the molds isolated from wooden printing blocks in Janggyeong Panjeon of Haeinsa temple in Korea and bacteria isolated from Ola leaf are cultured. After spreading of microorganisms suspension on agar plate, a disk paper containing certain volume of herbal oil is put on the agar plate. From the experiment, it is found that the herbal oil exhibits a clear zone, which is optically clear and inhibits growth of microorganisms, against some molds and bacteria. The result indicates that herbal oil from the plant Halvateria acuminata has the anti-bacteria and anti-fungal properties. Keywords: herbal oil, anti-bacteria, anti-fungal The Korean Society for Microbiology and Biotechnology 403
J-32 Enhancement of Biohydrogen Production in [NiFe]-hydrogenase Expressing Recombinant Escherichia coli by Introducing Light Acceptor Younghwa JO, Jaoon Y.H. KIM, Byung Hoon JO and Hyung Joon CHA* Pohang University of Science and Technology, Pohang 790-784, Korea. *Corresponding author: hjcha@postech.ac.kr Biohydrogen production can be one of solutions for energy crisis. Previously, we successfully demonstrated the production of biohydrogen by constructing [NiFe]-hydrogenase-expressing recombinant E. coli. Although [NiFe]-hydrogenase showed relatively high oxygen-tolerance compare to other hydrogenases, still biohydrogen production efficiency was low. In this research, we introduced proteorhodopsin, light acceptor for prokaryote working as proton pump, into [NiFe]-hydrogenaseexpressing recombinant E. coli for utilizing light energy for producing biohydrogen. Proteorhodopsin need a chemical named retinal for working as proton pump by using light energy but E. coli does not have a pathway to synthesize it. In order to give E. coli an ability to synthesize retinal, we expressed five foreign genes expressing enzymes for retinal synthesis pathway. By introducing light accepting system into E. coli expressing [NiFe]-hydrogenase, we could get enhanced biohydrogen productivity. Keywords: Biohydrogen, [NiFe]-hydrogenase, proteorhodopsin Novel Probiotic Strains for American Foulbrood and Nosemosis of Honeybee Yoon Jeong JANG 1 , Byoung Joo SEO 1 , Irfan RATHER 1 , Uk Han CHOI 1 , Jeonghuei LIM 1 , Kook Hee KANG 2 , Byoung Su YOON 3 and Yong-Ha PARK* 1 J-33 1 Department of Applied Microbiology and Biotechnology, Yeungnam University. 2 3 Korea Institute of Science and Technology Information, Daejeon. Department of Life Science, Kyonggi University, Suwon, Kyonggi-Do. *Corresponding author: joos02@ynu.ac.kr American Foulbrood of honeybees (Apis mellifera), caused by the Gram-positive bacterium Paenibacillus larvae is one of the most serious diseases affecting the larval and pupal stages of honeybees. Presently, Nosema ceranae and Nosema apis seems to be the main microsporidian infection in honeybees. The aim of this study was to evaluate lactic acid bacteria strains isolated from Kimchi and to study its inhibitory effects against Paenibacillus larvae subsp. larvae KCTC 3744 and Nosema trichoplusiae ATCC 30702. In all, 3,500 strains were screened and three highly active bacteria were selected and were identified as L. plantarum YML001, L. plantarum YML004, Leu. citreum KM20. The optical density of P. larvae KCTC 3744 at OD600nm showed drastic decrease in cell growth as compared to control. Three groups of honey bees (N=25) were given Nosema Spores at a concentration of 10,000 spores per ml with addition of 1% of supernatant of three strains. The bees were incubated at 37°C and samples were taken seven days post infection, and the amount of spores in the mid gut was counted. We found that the three strains inhibited the growth of P. larvae KCTC 3744 and N. trichoplusiae ATCC 30702 and provided new perspective for probiotics of honeybees. Keywords: American Foulbrood, Nosemosis, Probiotics 404 www.kormb.or.kr Analysis of Bacterial Community in Electrochemical Bioreactors Cultivated with Carbon Dioxide as Sole Carbon Source Doo Hyun PARK 2 , Jinsu KIM 2 , Bo Young JEON 2 and Il Lae JUNG* 1 1 Department of Radiation Biology, Environmental Radiation Research Group, Korea Atomic Energy Research Institute, Daejeon 305-353, Korea. 2 J-34 Department of Biological Engineering, Seokyeong University, Seoul 136-704, Korea. *Corresponding author: baakdoo@skuniv.ac.kr Bacterial community was isolated from different sources that were forest soil, aerobic wastewater treatment reactant, anaerobic wastewater digestive reactant, farm field soils, mixture of isolated bacterial strains. Bacterial communities grown in the different bioreactors were analyzed by temperature gradient gel electrophoresis technique. Different bacterial communities were enriched and selected during cultivation with the electrochemical reducing power as a sole energy source. TGGE pattern is not an indicator to show all of the bacterial communities grown in the electrochemical bioreactors; however, the bacterial species capable of growing with carbon dioxide or surviving in the condition saturated with carbon dioxide may be enriched selectively. According to the bacterial species identified based on the sequence homology, various bacterial species may fix metabolically carbon dioxide. Acknowledgement This work was supported by the New &Renewable Energy of the Korea Institute of Energy Technology Evaluation and Planning (KETEP) grant funded by the Korea governmental Ministry of Knowledge Economy (2010T1001100334) Keywords: electrochemical reducing power, carbon dioxide fixation, Bacterial community diversity Screening of Protease-Producing Bacteria from Gastropods Jung-Hee WOO* 1 , Sun Mee HONG 1 , Jun-Tae KIM 1 , Jin-Wook LEE 1 , Hyun-Soo RHO 2 , Jong-Shik KIM 1 , Nyun-Ho PARK 1 and Choong-Gon KIM 1 1 1Gyeongbuk Institute for Marine Bio-Industry (GIMB), Uljin 767-813, Gyeongbuk, Korea. 2 J-35 2East Sea Research Institute, Korea Ocean Research &Development Institute (KORDI), Uljin 767-813, Gyeongbuk, Korea. *Corresponding author: jhwoo@gimb.or.kr Microbial proteases are the most important group of industrial enzymes such as food, detergent, pharmaceutical, and waste management industries. Previously we reported that bacteria isolated 127 strains from six gastropods F. oregonensis, N. cumingi, N. constricta, C. fulmen, V. emphaticus, JG in June to December 2010 were studied to find out the bacterial assemblages associated with local gastropod collected in Uljin, the East Sea, Korea. Protease-producing bacteria were isolated from gastropods. To screen for proteolytic activity, various agar medium supplemented with 0.5% skim milk were used. We found that 38 isolate strains showed proteolytic activity on skim milk agar plate (10 o C = 16 strains, 25 o C = 22 strains). This study presents a first example which discovered microbial proteases from gastropods successfully. [This work was supported by the Marine and Extreme Genome Research Center Program, Ministry of Land, Transport and Maritime Affairs, Republic of Korea] Keywords: Protease, Proteolytic activity, Gastropods
Page 1 and 2: Isolation and Characterization of L
Page 3 and 4: Characterization of Phosphate-Solub
Page 5 and 6: Thermodynamic and Kinetic Analyses
Page 7: Identification of Novel Sugar Pathw
Page 11 and 12: Bioelectrochemical Fixation of CO2
Page 13 and 14: Inhibition of Quorum Sensing-Contro
Page 15 and 16: J-56 Development of Baeyer-Villiger

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