Pesticides-Online and Method Validation Database - EURL ...
Pesticides-Online and Method Validation Database - EURL ...
Pesticides-Online and Method Validation Database - EURL ...
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<strong>Pesticides</strong>-<strong>Online</strong> <strong>and</strong><br />
<strong>Method</strong> <strong>Validation</strong> <strong>Database</strong><br />
Two <strong>Database</strong> Modules to Access Pesticide-Related Data via the Internet<br />
Dr. H. Zipper<br />
Chemisches und Veterinäruntersuchungsamt Stuttgart<br />
EU-CRL for Pesticide Residues using Single Residue <strong>Method</strong>s<br />
Test<br />
Version<br />
<strong>Online</strong>!
CIRCA<br />
Document<br />
Management<br />
System<br />
CRL-FV<br />
Fruits & Vegetables<br />
Member-<br />
Area<br />
[restricted<br />
access]<br />
CRL-CF<br />
Cereals & Feeding Stuff<br />
CRL-<strong>Pesticides</strong>-Website<br />
www.???.eu<br />
[= website for experts]<br />
CRL-FAO<br />
Food of Animal Origin<br />
→ www.pesticides-online.com<br />
→ <strong>Method</strong> <strong>Validation</strong> <strong>Database</strong><br />
CRL-SRM<br />
Single Residue <strong>Method</strong>s<br />
Two <strong>Database</strong> Modules to Access Pesticide-Related Data:<br />
2CRL for<br />
Residues of <strong>Pesticides</strong> SRM
Targeted Sampling & Analysis<br />
High-Tech-Instrumentation & Fast Sample Preparation<br />
are Important, but...<br />
Important is not only<br />
How You Analyse<br />
but also…<br />
What You Analyse!<br />
Set priorities based on information...<br />
3CRL for<br />
Residues of <strong>Pesticides</strong> SRM
Targeted Analysis<br />
Relevant spectrum<br />
Pome Fruit<br />
Relevant spectrum<br />
Berries<br />
… using SRM<br />
Laboratory spectrum<br />
using the MRM<br />
Entire spectrum<br />
of pesticides<br />
Potential Spectrum<br />
of the MRM<br />
4CRL for<br />
Residues of <strong>Pesticides</strong> SRM
Targeted Analysis<br />
Relevant spectrum<br />
Pome Fruit<br />
Relevant spectrum<br />
Berries<br />
… using SRM<br />
Laboratory spectrum<br />
using the MRM<br />
Entire spectrum<br />
of pesticides<br />
Potential Spectrum<br />
of the MRM<br />
5CRL for<br />
Residues of <strong>Pesticides</strong> SRM
DATA-POOL: Collection/Distribution of Information<br />
Lab 3<br />
Lab 2<br />
Lab 4<br />
Lab 1<br />
Common<br />
Data-Pool<br />
Lab 5<br />
INTERNET<br />
Lab 7<br />
Lab 6<br />
6CRL for<br />
Residues of <strong>Pesticides</strong> SRM
Aims:<br />
Discussion-Forum<br />
PESTICIDES-ONLINE<br />
- Information Exchange between Laboratories<br />
- Enable Targeted Pesticide-Analysis<br />
Pesticide-<br />
Data-Sheets<br />
Pesticide-Usage<br />
Data<br />
7CRL for<br />
Residues of <strong>Pesticides</strong> SRM<br />
Residue Findings
www.pesticides-online.com<br />
Navigation bar<br />
Navigation bar<br />
8CRL for<br />
Residues of <strong>Pesticides</strong> SRM
Registration to <strong>Pesticides</strong>-<strong>Online</strong><br />
<strong>Online</strong>-Registration is necessary!<br />
After Registration only the Forum is can be used.<br />
Administrator will contact you by email <strong>and</strong> give you an access!<br />
The access is free of charge.<br />
Fill-in the requested<br />
information <strong>and</strong><br />
submit.<br />
Different access rights to <strong>Pesticides</strong>-<strong>Online</strong>:<br />
- Full access for official labs/organisations, labs/organisation that<br />
submit data (e.g. residue findings)<br />
- Limited access for labs/organisations that submit no data<br />
9CRL for<br />
- No access for NGO, journalists, private individuals, … SRM<br />
Residues of <strong>Pesticides</strong>
<strong>Pesticides</strong>-<strong>Online</strong> Users Around the World:<br />
10CRL for<br />
IS<br />
1<br />
UK<br />
14<br />
BE<br />
10<br />
NL D<br />
12 193<br />
LV<br />
DK SE PL FI EE<br />
1<br />
3 6 9 3 3<br />
CZ<br />
4<br />
SK<br />
6 HU<br />
3<br />
USA<br />
30<br />
IE<br />
2<br />
F<br />
13<br />
PT<br />
4<br />
ES<br />
29 MA<br />
3 CH<br />
29<br />
BR<br />
7<br />
UY<br />
AT IT<br />
7 31<br />
GH<br />
2<br />
SI<br />
3<br />
GR<br />
8<br />
RO<br />
1<br />
TR<br />
13<br />
RL<br />
1<br />
IL<br />
1<br />
SD CY<br />
1 1<br />
BG<br />
5<br />
KR<br />
3<br />
JP<br />
19<br />
CN<br />
2<br />
IN<br />
1<br />
CL<br />
3<br />
2<br />
AR<br />
3<br />
Residues of <strong>Pesticides</strong><br />
ZA<br />
1<br />
AU<br />
SRM<br />
2<br />
Currently 506 users from 53 countries
Various filter options<br />
for a targeted query<br />
View reference <strong>and</strong> contactinformation<br />
by clicking here<br />
Sorting<br />
options<br />
for the<br />
results<br />
11CRL for<br />
Residues of <strong>Pesticides</strong> SRM
<strong>Pesticides</strong>-<strong>Online</strong> - Statistics<br />
Residue Data<br />
>1 000 000 Entries<br />
(> 75 000 pos.)<br />
314 Different Commodities<br />
116 Countries of Origin<br />
678 Compounds Sought<br />
512 Compounds Found<br />
91 Info Sources (35 labs)<br />
12CRL for<br />
Residues of <strong>Pesticides</strong> SRM<br />
80000<br />
70000<br />
60000<br />
50000<br />
40000<br />
30000<br />
20000<br />
10000<br />
0<br />
4 5 6 7 8 9<br />
min
Results of Lab-Questionnaire 2006<br />
Website-section of lab-questionnaire:<br />
What types of data would you like to see in our common CRL website?<br />
Would you be willing to contribute data to such a database<br />
(= RESIDUE FINDINGS <strong>Database</strong>)?<br />
%<br />
70<br />
60<br />
50<br />
40<br />
30<br />
20<br />
10<br />
0<br />
61<br />
13CRL for<br />
Residues of <strong>Pesticides</strong> SRM<br />
1<br />
(71 answers<br />
to this questions)<br />
Yes No Don`t know yet<br />
38
Data Submission to <strong>Pesticides</strong>-<strong>Online</strong><br />
For more convenience, we try to<br />
use the data-formats (residue<br />
findings) that our users are<br />
already utilizing in their lab <strong>and</strong><br />
translate them into ours.<br />
Thus, please send us an example<br />
of your data so that we can look at<br />
it <strong>and</strong> see what we can do.<br />
If you don't have a LIMS in your lab,<br />
we can also offer you a tool based<br />
on Microsoft Access to easily collect<br />
your pesticides residue data.<br />
Which information do we need at<br />
least?<br />
- Commodity (+ Processing)<br />
- Pesticide sought for + value<br />
(negatives also interesting!!!)<br />
- Year of analysis<br />
- Country of origin<br />
14CRL for<br />
- (Other: organic, MRL-violation)<br />
SRM<br />
Residues of <strong>Pesticides</strong>
Various filter options<br />
for a targeted query<br />
Sorting options<br />
for the<br />
query results<br />
View reference <strong>and</strong> contactinformation<br />
by clicking here<br />
15CRL for<br />
Residues of <strong>Pesticides</strong> SRM
<strong>Pesticides</strong>-<strong>Online</strong> - Statistics<br />
Agricultural Usage Data<br />
>16 000 Entries<br />
194 Commodities<br />
473 <strong>Pesticides</strong><br />
35 Countries/Regions<br />
16CRL for<br />
Residues of <strong>Pesticides</strong> SRM
Printing option<br />
Printing option<br />
Click here to see the<br />
„Pesticide Data Sheet“<br />
Physicochemical, Toxicological <strong>and</strong><br />
Analytical Information for<br />
795 <strong>Pesticides</strong> <strong>and</strong> Metabolites<br />
Sorting options<br />
for the<br />
results<br />
17CRL for<br />
Residues of <strong>Pesticides</strong> SRM
Physicochemical <strong>and</strong><br />
Toxicological Data<br />
Analytical Data<br />
Analytical Data<br />
18CRL for<br />
Residues of <strong>Pesticides</strong> SRM
Physicochemical data:<br />
- pKa<br />
- Water solubility<br />
- …<br />
Toxicological data:<br />
- ARfD<br />
- ADI<br />
19CRL for<br />
Residues of <strong>Pesticides</strong> SRM
LC-Behavior<br />
Typical recoveries<br />
with various<br />
multiresidue methods<br />
GC-Behavior<br />
20CRL for<br />
Residues of <strong>Pesticides</strong> SRM
Unfortunately the Forum is not much used up to now.<br />
But ...<br />
21CRL for<br />
Residues of <strong>Pesticides</strong> SRM
Results of Lab-Questionnaire 2006<br />
Would you like a discussion forum<br />
to be established?<br />
%<br />
100<br />
90<br />
80<br />
70<br />
60<br />
50<br />
40<br />
30<br />
20<br />
10<br />
0<br />
92<br />
(74 answers<br />
to this questions)<br />
Yes No<br />
Would you like the forum to be<br />
accessible also to colleagues outsite<br />
the CRL/NRL-Network (e.g.<br />
colleagues from countries outside<br />
the EU, from private labs...)?<br />
8<br />
Would you be willing to<br />
use such a forum?<br />
22CRL for<br />
0<br />
Residues of <strong>Pesticides</strong><br />
Yes No<br />
SRM<br />
Analytical labs Don`t know yet<br />
%<br />
%<br />
80<br />
70<br />
60<br />
50<br />
40<br />
30<br />
20<br />
10<br />
100,0<br />
90,0<br />
80,0<br />
70,0<br />
60,0<br />
50,0<br />
40,0<br />
30,0<br />
20,0<br />
10,0<br />
0,0<br />
72<br />
91,4<br />
7,1<br />
(70 answers<br />
to this questions)<br />
1,4<br />
Yes No No, due to lack of<br />
time, not lack of<br />
interest<br />
23<br />
1<br />
(69 answers<br />
to this questions)<br />
3
<strong>Method</strong> <strong>Validation</strong> <strong>Database</strong><br />
New project started this year:<br />
Test<br />
Version<br />
<strong>Online</strong>!<br />
→ <strong>Database</strong> for storage of data generated within<br />
the aim of method validation experiments in<br />
various laboratories.<br />
⇒ Enables the systematic collection <strong>and</strong><br />
customized retrieval of data produced during<br />
method validation in different laboratories.<br />
23CRL for<br />
Residues of <strong>Pesticides</strong> SRM
Registration to <strong>Method</strong> <strong>Validation</strong> <strong>Database</strong><br />
<strong>Online</strong>-Registration will be necessary!<br />
Fill-in the requested<br />
information <strong>and</strong><br />
submit.<br />
24CRL for<br />
Residues of <strong>Pesticides</strong> SRM
Query Section: Various filter options for a targeted query<br />
Filter options<br />
for commodity<br />
Filter options<br />
for extraction method,<br />
ISTD, Cleanup, …<br />
Filter options<br />
for compound<br />
Filter options<br />
for lab name, …<br />
25CRL for<br />
Residues of <strong>Pesticides</strong> SRM
1. Result list of individual recovery values<br />
Aggregation mode 1<br />
Aggregation mode 1<br />
N° of hits:<br />
N° of hits:<br />
All important experimental<br />
parameters are shown!<br />
Aggregation mode 2<br />
Aggregation mode 2<br />
26CRL for<br />
Residues of <strong>Pesticides</strong> SRM
2. List with aggregated recovery data<br />
Important experimental<br />
parameters are shown!<br />
Aggegation line for 2,4-D in Cucumber at Spiking level 0,01 mg/kg<br />
Aggegation line for 2,4-D in Raisin at Spiking level 0,01 mg/kg<br />
Aggegation line for all commodites at spiking level 0,01 mg/kg<br />
27CRL for<br />
Residues of <strong>Pesticides</strong> SRM
<strong>Method</strong> <strong>Validation</strong> <strong>Database</strong> - Statistics<br />
currently only data for QuEChERS-<strong>Method</strong>,<br />
but <strong>Method</strong> <strong>Validation</strong> <strong>Database</strong> is flexible to<br />
receive data from any other method!<br />
Recovery data for QuEChERS-<strong>Method</strong>:<br />
- 478 compounds<br />
- 44 commodities (apple, raisin, lemon, …)<br />
- 17 labs data submitted<br />
Recovery data of S19- <strong>and</strong> ChemElute will be<br />
submitted soon from different labs.<br />
You’re welcome to submit validation data from your lab!<br />
But how to submit?<br />
28CRL for<br />
Residues of <strong>Pesticides</strong> SRM
Data Submission to <strong>Method</strong> <strong>Validation</strong> <strong>Database</strong><br />
internet<br />
We offer a Data Submission-Format<br />
(→ Excel-file)<br />
If you are interested, please contact us!<br />
If you have huge amounts of recovery<br />
data, we will try to transform your data<br />
into our data-format.<br />
29CRL for<br />
Residues of <strong>Pesticides</strong> SRM
...our Pesticide Residue Team...<br />
Thank you very much<br />
for your Attention !<br />
30CRL for<br />
SRM<br />
Residues of <strong>Pesticides</strong>
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