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Indian Journal Of Natural Sciences International Bimonthly ISSN: 0976 – 0997 Vol.3 / Issue 14/ October2012 www.tnsroindia.org.in © IJONS Jesikha et al. 9. Bansal S and Kapoor K K. Vermicomposting of crop residues and cattle dung with Eisenia foetida. Biores. Technol 2000; 73: 95-98. 10. Kale R D. Vermicomposting has a bright scope, Indian Silk 1995; 34: 6-9. 11. Tajbakhsh J Abdoli M A Mohammadi Goltape, E Alahdadi I and Malakouti M J. Trend of physico-chemical properties change in recycling spent mushroom compost through vermicomposting by epigeic earthworms Eisenia foetida and E. andrei. Journal of Agricultural Technology 2008; 4(2): 185-198. 12. Levelle P.. Les vers de terre de la savane de Lamto (cote d’ lvoire). Peuplements, populations et fonctions de locosysteme. Publlab Zool ENS 1978; 12:1-301. 13. Sharma S Kaviraj Pradhan Santosh Satya and Padma Vasudevan. Potentiality of Earthworms for Waste Management and in Other Uses – A Review. The Journal of American Science 2005; 1(1): 4-16. 1152
Indian Journal Of Natural Sciences International Bimonthly ISSN: 0976 – 0997 Vol.3 / Issue 14/ October2012 www.tnsroindia.org.in © IJONS Isolation and Characterization of Polyhydroxyalkanoates (PHAs) Producing Bacteria from Sago Industrial Wastewater. Umamaheswari.S 1*, P. S Dheenan 2, S. Govindraj 1, Kishna Kumar.S 1, Sri Murali, S 1, Sri Priya.J 1 and R.Babu Rajendran 1 1Department of Environmental Biotechnology, School of Environmental Sciences of Bharathidasan University, Tiruchirappalli. Tamil Nadu, India 2Project Assoicate, Andaman and Nicobar Center for Ocean Science and Technology, NIOT R&D Complex, Industrial Estate Road End, Dollyguni, Port Blair. Received: 15 June 2012 Revised: 25 July 2012 Accepted: 25 Sep 2012 *Address for correspondence Umamaheswari.S Department of Environmental Biotechnology, School of Environmental Sciences, Bharathidasan University,Tiruchirappalli.Tamil Nadu,India. E.mail: myscienceworld@gmail.com ABSTRACT Polyhydroxyalkanoates (PHA’s) are biological polyesters that are produced by a wide variety of bacteria as an intracellular storage material of carbon and energy. Recently PHA has attracted considerable industrial attention because they are biodegradable and in many cases, their physico and mechanical properties compare favorably with synthetic polymers and are ecofriendly. For a developing country like India, use of cheap waste effluent production of PHA is quite attractive and interesting. In our study The Nile blue A staining was done to screen for PHAs producing bacteria followed by nucleotide sequencing which analysis further confirmed the bacterial strain, and finally these isolates were then subjected to identification and quantification of P(3HB-co-3HV) co polymer using GC-MS. These results demonstrate that the environmental bacterial stains were able to accumulate the PHA which was confirmed by Nile blue A staining method, and also GC-MS was used to quantify by the PHA produced by the strains were isolated from sago waste effluent. Five different forms were screened in which Bacillus sp was selected for further studies, i.e. for PHA production using different carbon and nitrogen sources. The paper will elucidate the primary screening of PHA production and characterization in the isolated strain Bacillus sp. Key words: Polyhydroxyalkanoates, Bacillus spp., Nile blue A staining, P(3HB-co-3HV) RESEARCH ARTICLE 1153
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