Tom Kirchhausen - CB201 - Harvard Medical School

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• assembly of nuclear pores it happens after nuclear membrane formation • formation of clathrin coated pits and coated vesicles: stochastic • initiation • growth • uncoating
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Tom Kirchhausen HARVARD MEDICAL SCH
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8 copies 8 copies
Page 6 and 7: histone H2 (chromatin) Sec61β (ER,
Page 8 and 9: mitotic ER
Page 10 and 11: tomographic EM reconstruction of mi
Page 12 and 13: assembly of nuclear pores IBB nucle
Page 14 and 15: intensity calibration f s all nucle
Page 16 and 17: • formation of clathrin coated pi
Page 18 and 19: Main routes of intracellular membra
Page 20 and 21: coated vesicles Heuser 1983 100 nm
Page 22 and 23: endocytosis of TfR plasma membrane
Page 26 and 27: Total Internal Reflec.on Fluores
Page 29 and 30: 1. initiation 2° step intensity di
Page 31 and 32: 0.61 clathrins @ initiation Frequen
Page 33 and 34: 1 2 3 ~ 2 sec igure 6. roposed mode
Page 35 and 36: AP-1(TGN, endosomes) & AP-2 (plasma
Page 37: capture of LDL by a forming clathri
Page 42 and 43: Uncoating lasts ~ 6 s and is Hsc70,
Page 44 and 45: where are auxilin and Hsc70? Fotin
Page 46 and 47: Clathrin Auxilin
Page 48 and 49: PTEN-like domain of auxilin: vesicl
Page 51 and 52: - Dynasore + Dynasore PIP’s auxil
Page 53 and 54: Single-molecule visualization of un
Page 55: mechanism of uncoating • signal (
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Tom Kirchhausen - CB201 - Harvard Medical School

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