Lipoprotein Lipase Activity in Skeletal Muscle Is Related to Insulin ...

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1196 Arteriosclerosis and Thrombosis Vol 11, No 5 September/October 1991 TABLE 2. Variable SBP supine SBP standing DBP supine DBP standing HR supine HR standing Blood Pressure Measurements Normal-weight control 135 + 18 128±18 79±7 79±10 64±11 70±ll for the Study Population Subgroup Obese normoinsulinemic 142±16 139±14 82±9 85 ±6 69±6 74±8 Obese hyperinsulinemic 149±13 152±14t 97±6* 99±10 + 67±8 79±5 Obese diabetic 145±11 144±llf 87±7 92±8* 71±8 82±9* Values are mean±SD for systolic (SBP) and diastolic (DBP) blood pressure (mm Hg) and heart rate (HR, beats/min) in the supine and standing positions for the four subgroups (n=7 in each group). For statistics, see footnote to Table 1. The steady-state levels of insulin that were reached during the euglycemic clamp test reflect the metabolic clearance rate of insulin. In our study as in others, 29 the mean steady-state insulin concentrations were higher in the obese hyperinsulinemic and diabetic groups (116±15 and 124±20 milliunits/1, respectively) than in the normoinsulinemic groups (85±15 and 87±18 milliunits/1, respectively), which is consistent with a lower rate of metabolic insulin clearance in the two hyperinsulinemic groups. To evaluate the degree to which hepatic glucose production was inhibited with the present hyperinsulinemic clamp procedure in this kind of subject, we studied hepatic glucose levels in two representatives from each group. The hepatic glucose output during the last hour of the clamp study and the percentage suppression of hepatic glucose production are shown for each group separately. In normal-weight controls (n=2), these values were 2.02±0.15 and 0.11±0.04 mg/kg body wt/min and 95%, respectively; in the obese normoinsulinemic group (n=2), 2.29±0.10, 0.14±0.04, and 94%; in the obese hyperinsulinemic group (n=2), 2.62±0.20, 0.20±0.03, and 92%; and in the obese diabetic group (n=3), 2.87±0.10,0.34±0.02, TABLE 3. Serum Lipid Values for the Study Population Subgroup Normal-weight Obese control normoinsulinemic Variable Cholesterol (mmol/l) Serum VLDL chol LDL chol HDL chol HDLj chol HDL, chol 5.53±0.77 0.35±0.22 4.11±0.74 1.05+0.29 0.40±0.28 0.64±0.04 5.87±1.07 0.66 ±0.70 4.18±1.41 1.00±0.16 0.28±0.14 0.72±0.14 Obese hyperinsulinemic 5.76±0.87 0.90±0.59* 3.94±0.54 0.86±0.16* 0.25±0.11 0.61 ±0.07 Obese diabetic 5.88±1.01 0.93±0.56* 4.01 ±0.94 0.93±0.10 0.30±0.10 0.63±0.12 Trigfycerides (mmol/l) Serum VLDLTG LDLTG HDLTG HDL2TG HDL3TG Plasma FFA Plasma glycerol 1.42±0.59 0.72±0.44 0.49+0.15 0.17±0.06 0.08±0.05 0.09±0.05 0.41 ±0.18 0.11+0.04 2.14±1.51 1.35±1.31 0.51±0.21 0.20±0.07 0.06±0.09 0.14±0.06 0.50±0.14 0.13±0.05 2.91±1.21* 2.05±0.92* 0.53 ±0.25 0.24±0.09* 0.09±0.05 0.15±0.04* 0.50±0.10 0.15±0.02* 3.02±1.28* 2.17±1.26* 0.54±0.10 0.26±0.07* 0.08 ±0.09 0.18±0.08t 0.82 ±0.24* 0.20±0.03* Other variables Urate ALAT GT 333 ±63 0.32±0.14 0.29±0.21 365+42 0.40±0.09 0.54±0.22 434±155 0.73 ±0.21* 0.60±0.22 326 ±77 0.66±0.8t 0.71 ±0.26* Values are mean±SD for serum lipoprotein, plasma free fatty acid, plasma free glycerol, and urate concentrations and for ALAT and GT enzyme activities for the four study groups (n=7 in each group). For statistics, see footnote to Table 1. VLDL, very low density lipoprotein; chol, cholesterol; LDL, low density lipoprotein; HDL, high density lipoprotein; TG, triglycerides; FFA, free fatty acids; ALAT, alanine aminotransferase; GT, glutamyltransferase. Downloaded from http://atvb.ahajournals.org/ by guest on August 29, 2013
Pollare et al LPL Activity and Insulin Sensitivity 1197 Insulin sensitivity index 10 1 9 8 7 6 5 4 3 2 1 f * * _ 1 * * 4 II 1 1 1 100 % 1 2 3 4 FIGURE 1. Bar graph showing glucose infusion rate (mg/kg body wt/min/milliunitxlOO; adjusted for prevailing insulin concentration) during the last hour of the clamp test in the four study groups: 1) normal-weight, normoinsulinemic, normoglycemic control group; 2) obese, normoinsulinemic, normoglycemic group; 3) obese, hyperinsulinemic, normoglycemic group; and 4) obese, hyperinsulinemic diabetic group. **p
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