Sick - Johns Hopkins Medicine

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X 1. Some Sexual Dimorphisms Size growth curves = NICE DATA ! • Are the GEM really smaller? Slower growing ? Anogenital distance –tough in younger • In utero influences Nipples –not in males Salivary glands – bigger in males • Acidophilic tubules Kidneys – bigger in females ? Males ? • Cuboidal parietal epithelium –male (>> female) Adrenal glands – bigger in females • X zone (vacuolar change in females) 11 1. Some Sexual Dimorphisms If they’re normal, what’s the big deal? Are there sexual dimorphisms in your data, or research endpoints ? ANALYZE Male & Female data separately ALSO CONSIDER: NOT having these may be an important phenotype 12 Brayton - NON infectious - P 2 Sex and SIZE (+ Genetic Background) SIZE (body weight) M > F B6D2F1 ~ Hi? C3H/HeJ ~ lo Jax Phenome Database for physiological data by strain sex age protocol – Jax Pheno1 – n = 5‐15 – 6% fat diet NIH 316 Purina 5K52 13 ♂ ♀ Sexual Dimorphisms Which is Male? A or B ? Check Anogenital distance Nipples ♀ ♂ A B Sexing pups Nice pictures at http://www.thefunmouse.com/info/sexing.cfm Salivary Glands, associated Nodes Which is Male ? A A or B ? 1. Submandibular 2. Sublingual B 3. Parotid (exorbital lacrimal) 4. Lymph nodes 14 Nipples may be evident by about 6do 16 A Tissues? Which is Male? B Tissue? Which is more likely to be male? A B A FEMALE Tissue? Which is Female ? B cbrayton@jhmi.edu 2013 17 18 19
X 1. Sexual Dimorphisms Size Growth Curves = NICE DATA ! Salivary glands, Kidneys, Adrenal glands etc Many More CONCLUSIONS: EXPECT THE EXPECTED –consider the unexpected ANALYZE Male & Female data separately Missing Dimorphisms may be a phenotype 20 X 2. 2 Young Mouse Phenotypes to identify by / at weaning: 1. Hydrocephalus • Later onset not evident at weaning E.g. sporadic in MRL etc. 2. Malocclusion / Incisor overgrowth • Cull or trim • Breed?? 3. Microphthalmia • Expect this in C57BL – but it won’t kill them • Especially? Right eye in females 21 22 Hydrocephalus Especially C57BL Brayton - NON infectious - P 3 Malocclusion Phenotypes 6g –6 weeks old Developmental delay’ Early Death 23 X 2. 2 Young Mouse Phenotypes to identify by / at weaning: 1. Hydrocephalus 2. Malocclusion / Incisor overgrowth 3. Microphthalmia etc CONCLUSIONS: EXPECT THE EXPECTED –consider the unexpected DON’T let these kill your mice ! Examine your mice – weaning is a good time to weigh them too 24 X 3. 3 Common Clinical Complaints 1) Skin Disease 1) Barbering –more common 2) Ulcerative Dermatitis –more serious 3) Flakey skin ‐ in immunodeficient – discussed later 4) Tumors – discussed later 5) Mites – discussed frequently 2) Abdominal distention 1) Pregnancy 2) Hydrometra mucometra pyometra 3) Hydronephrosis (MUS) 4) Neoplasia – effusion, organomegaly 5) Effusion ‐ Neoplasia ? Heart or kidney disease? 253) Neurologic signs 1 st Clinical Complaint: Skin cbrayton@jhmi.edu 2013 26 Alopecia – Social phenomenon F > M – Vibrissae are important in some behavior tests … Skin Alopecia Barbering – Strain/sex specific patterns – Nibbled • Not plucked – ouch! – Vibrissae are important in some behavior tests … Kalueff 27 et al. 2006. 129S1 ♂ NMRI ♂ C57Bl/6 ♂ C57Bl/6 ♀ NMRI ♀ A/J ♂ ♂ X SKIN: ulcerative dermatitis Mouse Ulcerative Dermatitis (MUD) • Interferes with a lot of research • Secondary phenotypes: Leukocytosis Lymphadenomegaly, Splenomegaly Amyloidosis, GN <strong>Sick</strong> mice Mortality C57BL > others 28
Page 1: Sick (& not so sick) Mice NON Infec
Page 5 and 6: X 38 1. Skin Disease 3. 3 Common Cl
Page 7 and 8: 56 Lymphomas Hematopoietic Tumors
Page 9 and 10: X Mouse Mammary Tumors Spontaneous
Page 11: Teratoma in B6, 129 chimera Swollen

Sick - Johns Hopkins Medicine

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