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After mixing thoroughly-the pH will be adjusted to 5.8 and the batch autoclaved<br />
@ 121 C. for 15 minutes<br />
The Future<br />
The future is already here! Research with DNA using PCR [Polymerase chain<br />
reaction][U.K.] has opened our ability to see the entire helix, including detection<br />
of genetic mutations, detection of food-borne pathogens, forensic applications,<br />
determination of relationships among plant species, and possibilities of plant/<br />
animal fusion. Research in "t" cell area is facilitated.<br />
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It is postulated that ivy can become even more useful than just covering walls.<br />
The DNA can be manipulated to include 'Bt' [Bacillus thuringensis, often<br />
called "Milky Spore] which has an affinity for soft, crawling insects. A chemical<br />
corporation has successfully engineered Bt in the DNA of corn seed to<br />
control borers in corn plants. The "Bt" has been engineered to become part<br />
of the corn DNA helix to target borers. To protect the walls of wood from<br />
living borer intrusion they could plant decorative ivy with Bt to control the<br />
insect problem. Commercially this could be successful.<br />
References<br />
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PCR Technologies by Griffin and Griffin<br />
Experiments in PT Cultures by Dodds and Roberts<br />
Extensive Overviews by Sulgrove [in <strong>Ivy</strong> Bulletins]<br />
Tissue Cultures Techniques by Torres<br />
Gray's Manual of Botany Merck's Index<br />
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