14 Bioaccumulation - Info.pdf - haspi

Materials Supplies needed for 10 lab groups
Lab 14a: Biomagnfication
Supply
Provided (P) or
Needed (N)
Quantity
Very small container – plankton P 20
Small container – krill P 10
Medium container – small fish P 6
Medium-large container – tuna P 3
Large container – human P 1
Plastic beads P 100
Graph paper N 40
Lab 14b: Bioaccumulation of Toxins
Supply
Provided (P) or
Needed (N)
Quantity
Dialysis tubing (2 – 6” sections) P 10’
Starch solution P 200 ml
Contaminant A (food coloring) P 20 ml
Contaminant B (tincture of iodine) P 20 ml
Graduated cylinder N 1
Paper towels N 10
Beakers N 20
String – 6”-12” pieces N 40
IMPORTANT: Check the MSDS for safety information on unfamiliar chemicals
Additional Information
Each of the containers has already been labeled. The plastic beads each represent a
molecule of mercury. Add 5 beads to each of the plankton containers (the smallest ones),
before the start of activity 14a.
It is recommended that you purchase and use string to close the dialysis tubing. This way,
dialysis tubing can be reused for additional lab activities as long as it is not too stained from
the procedure.
Contaminants A and B will need to be passed around the classroom to “contaminate” the water
since only one bottle of each will be provided.
Kit Replenishment Instructions- HASPI Supported Sites Only
Funds permitting, HASPI will replace annually:
Dialysis tubing
Laundry starch solution (MUST return bottle for refill)
Contaminant A and B (MUST return bottles for refill)
Not being replaced annually, please recycle and reuse:
ALL containers
Plastic beads
HASPI Medical Biology Lab 14, Bioaccumulation, Teacher Info; Revised July 2011 198