Basics and Theory of Electrophoresis
Basics and Theory of Electrophoresis
Basics and Theory of Electrophoresis
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Sample Types<br />
Nucleic Acids <strong>and</strong> <strong>Electrophoresis</strong><br />
DNA is negatively charged (due to<br />
phosphate backbone).<br />
<strong>Electrophoresis</strong> <strong>of</strong> double str<strong>and</strong>ed DNA<br />
occurs under native gel conditions.<br />
<strong>Electrophoresis</strong> <strong>of</strong> single str<strong>and</strong>ed DNA<br />
occurs under denaturing conditions<br />
(ensures single str<strong>and</strong>ed molecules <strong>and</strong><br />
prevents base pairing).
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