Tech Note: Rapid and Comprehensive Screening for ... - AB Sciex
Tech Note: Rapid and Comprehensive Screening for ... - AB Sciex
Tech Note: Rapid and Comprehensive Screening for ... - AB Sciex
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Data from the dedicated positive <strong>and</strong> negative experiment were<br />
compared to data from the polarity switching experiment. Peak<br />
areas <strong>and</strong> signal-to-noise (S/N) were compared, as well as %CV<br />
of the peak areas.<br />
Intensity, cps<br />
Intensity, cps<br />
Intensity, cps<br />
Intensity, cps<br />
XIC of +MRM (78 pairs): 195.5/13... Max. 2.3e4 cps.<br />
2.0e4<br />
1.0e4<br />
(+) Caffeine<br />
6.9<br />
1.9e4<br />
(+/-)Caffeine<br />
S/N = 24.7 1.0e4 S/N = 20.0<br />
0.0<br />
5.0 5.5 6.0 6.5 7.0 7.5<br />
Time, min<br />
8.0 8.5<br />
XIC of +MRM (78 pairs): 235.2/86... Max. 1.1e6 cps.<br />
1.00e6<br />
5.00e5<br />
(+) Lidocaine<br />
6.5<br />
1.0e6<br />
(+/-) Lidocaine<br />
S/N = 705<br />
5.0e5<br />
S/N = 922<br />
0.00<br />
4.5 5.0 5.5 6.0 6.5 7.0<br />
Time, min<br />
7.5 8.0 8.5<br />
XIC of +MRM (78 pairs): 213.1/72... Max. 9.1e4 cps.<br />
9.1e4<br />
5.0e4<br />
0.0<br />
9.0 9.5 10.0 10.5 11.0<br />
Time, min<br />
11.5 12.0 12.5<br />
XIC of -MRM (33 pairs): 312.9/15... Max. 2.8e5 cps.<br />
2.8e5<br />
2.0e5<br />
1.0e5<br />
0.0<br />
Variations between the sets of data were minimal, demonstrating<br />
that no significant loss of data quality was observed when a<br />
positive/negative switching method was used versus a dedicated<br />
method (see Figure 1-4).<br />
Dedicated polarity Positive negative switching<br />
XIC of +MRM (78 pairs): Exp 1, 1... Max. 1.9e4 cps.<br />
0.0<br />
5.0 5.5 6.0 6.5 7.0 7.5<br />
Time, min<br />
8.0 8.5<br />
XIC of +MRM (78 pairs): Exp 1, 2... Max. 1.0e6 cps.<br />
Figure 3. Comparison of S/N <strong>for</strong> representative analytes: Data from the dedicated positive or negative experiment is shown on the left <strong>and</strong> data acquired<br />
using a positive/negative switching experiment is shown on the right. No significant degradation of data quality was observed when using the polarity<br />
switching experiment<br />
Intensity, cps<br />
Intensity, cps<br />
6.9<br />
6.5<br />
0.0<br />
4.5 5.0 5.5 6.0 6.5 7.0<br />
Time, min<br />
7.5 8.0 8.5<br />
XIC of +MRM (78 pairs): Exp 1, 2... Max. 9.1e4 cps.<br />
10.7<br />
(+) Chlorotoluron<br />
9.1e4<br />
10.7<br />
(+/-) Chlorotoluron<br />
S/N = 52.6 5.0e4 S/N = 48.8<br />
11.5 12.0 12.5 13.0 13.5 14.0 14.5 15.0<br />
Time, min<br />
Intensity, cps<br />
0.0<br />
9.0 9.5 10.0 10.5 11.0<br />
Time, min<br />
11.5 12.0 12.5<br />
XIC of -MRM (33 pairs): Exp 2, 3... Max. 1.9e5 cps.<br />
(-) Triclocarban<br />
13.3<br />
1.9e5<br />
13.3<br />
(+/-) Triclocarban<br />
S/N = 869<br />
1.0e5<br />
S/N = 592<br />
Intensity, cps<br />
0.0<br />
11.5 12.0 12.5 13.0 13.5 14.0 14.5 15.0<br />
Time, min<br />
p 3
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