Hojjatie - AAPFCO
Hojjatie - AAPFCO
Hojjatie - AAPFCO
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AOAC Official Method 2003.14<br />
Liquid Chromatography<br />
First Action 2003<br />
HPLC Method<br />
<br />
<br />
<br />
<br />
<br />
UV absorption detector 195 nm<br />
Mobile Phase: 15% Acetonitrile, 85% water<br />
Flow rate: 1.30 ml/min<br />
Mobile phase temp: Ambient<br />
Column temperature: 30-35 o C<br />
Injection volume: 10 µl<br />
<br />
<br />
HPLC Column – A 250 x 4.6 mm amine (NH2) column<br />
with 5µ packing is used for this separation We<br />
obtained the best results using Phenomenex amine<br />
column.<br />
External or standard curves be used (Urea, Biuret,<br />
MMU, DMU, and HMTA Standards).
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