Ticks research CIRAD - Caribvet
Ticks research CIRAD - Caribvet
Ticks research CIRAD - Caribvet
Create successful ePaper yourself
Turn your PDF publications into a flip-book with our unique Google optimized e-Paper software.
Ehrlichia ruminantium functional genomic<br />
• Genomic<br />
– Genome publications & patents: 2 strains of Ehrlichia ruminantium<br />
Sequencing & annotation: 3 additional strains virulent/attenuate<br />
development of tools for diagnostic and characterisation<br />
comparative genomic and functional genomic<br />
• Transcriptomic<br />
– Comparison between virulent and attenuate strains<br />
– Comparison between stages of development<br />
– ER = Obligate intracellular pathogen<br />
Adaptation of SCOTS method to capture bacterial transcripts<br />
<br />
gDNA<br />
RM<br />
SCOTS<br />
0x<br />
96Hpi<br />
SCOTS<br />
3x<br />
Microarrays and qRT-PCR validation:<br />
- ~30 genes discriminate virulent/attenuate strains<br />
- genes differentially expressed or presence/absence<br />
significativity<br />
Identification of pathogenesis mechanisms<br />
- virulence & adaptation to host cells, attenuation.<br />
<br />
Development of efficient recombinant vaccines<br />
variance
Change language