Preliminary phytochemical screening and Evaluation of Anti ...

Journal of Current Pharmaceutical Research 2010; 3(1): 47-50
been reported in this regard. In the present study, sincere effort has
been attempted to establish the scientific validity of the antiinflammatory
activity of the aerial part of plant.
2. MATERIALS AND METHODS
Plant material
The fresh leaves of the plant Indigofera tinctoria Linn.
were collected from Budhara, Porsa and campus of Shri R.N.S
College of Pharmacy Gormi, Bhind (M.P.). It was further
authenticated by taxonomist Dr. A. N. Rai, department of Botany,
Dr. H.S. Gour University, Sagar,M.P. The leaves were cut into
small pieces and shade dried at room temperature, dried leaves was
subject to size reduction to a coarse powder by using a dry grinder.
Chemicals
Carrageenan was purchased from Himedia Laboratories
Pvt. Ltd., Mumbai and Ibuprofen was obtained from Glenmark
Pharmaceutical Ltd., Mumbai as a gift sample.
Animal experiments
Wister albino rats weighing 200±20 g were taken from
Shri R.N.S College of Pharmacy, Gormi, Bhind (M.P.) and
maintained under constant condition (temperature: 20±2 o C,
humidity: 40-60% , 12-h light/ 12-h dark cycle) for two weeks and
housed in stainless steel cages in a laboratory room. The animals
were access to water ad libitum and standard rodent diet. All
animal experiment conducted in accordance with Institutional
Animal Ethical Committee (IAEC licence
no1030/A/07/CPCSEA.).
Table 1. Phytochemical screening of Petroleum ether & ethanolic
extracts of Indigofera Tinctoria linn.
Preparation of plant Extract
The course powder plant material was extracted with
petroleum ether (60-80 o C) for 72 hours and then using ethanol in
soxhlet extractor for 48 hours (Harbosne et.al., 1984). Alcoholic
extract was concentrated under the vacuum in rotary flash
evaporator and successively in hot air oven till solid to semisolid
mass. Extracts were stored in an airtight container in refrigerator
below 10 o C.
Preliminary phytochemical investigations:-
The petroleum ether extract and ethanolic extract
subjected to preliminary qualitative chemical analysis. Standard
method were used for preliminary phytochemical screening of the
extract was performed to know the phytoconstituents in the extract
.(Kokate. et.al., 2007, Khandelwal et.al.,2004), and it was found
that extract contains carbohydrate, glycosides, proteins, steroid,
amino acids, tannins and flavoniods.
Table 2. Physical contents of leaves of Indigofera Tinctoria linn.
S. NO. PARAMETERS PERCENTAGE
1. Total ash 12.5%
2. Acid insoluble ash 3.5%
3. Water soluble ash 9%
4 Sulphated ash 20%
5. Extractive value 14.2%
6. Loss on drying 6.9%
S.
NO.
PHOTOCHEMICAL
CONSTITUENTS
EXTRACT
PETROLEUM
ETHER
1. Alkaloids - -
2. Fats and oils + -
3. Carbohydrates - +
4. Glycosides + +
5. Steroids + +
6. proteins - +
7. Amino acid - -
8. Tannins - +
9. Flavonoides - +
ETHANOL
Determination of physical constants:-
Leaves analysis parameters like total ash, acid insoluble
ash, water soluble ash, Sulphated ash, loss on drying were use for
the determination of quality and purity of the crude drug and
extractive value were used for evaluation and study of antiinflammatory
activity (Evans et .al., 2002) .
Ethanolic extract of leaves of Indigofera tinctoria linn
(250, 500 & 1000 mg/kg bwt, p.o.) were prepared in tween 80
(1%w/v solution).
Carrageenan induced paw oedema in rats
Pedal inflammation in hind paw of Wister albino rats
(200±20 g) was produced according the method described (by
Winter,et.al., 1962). Oedema was induced by sub-planter injection
(+) represent presence & (-) absence of respective components
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