Protein and nucleic acid sample preparation - Whatman
Protein and nucleic acid sample preparation - Whatman
Protein and nucleic acid sample preparation - Whatman
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Nucleic Acid <strong>and</strong> <strong>Protein</strong> Workflows<br />
Nucleic Acid Workflow<br />
Genome <strong>and</strong> Gene Analysis<br />
<strong>Protein</strong> Workflow<br />
Differential Expression Analysis<br />
<strong>Protein</strong> Mapping<br />
Sample(s): blood, tissue, cells, bacteria, yeast, etc.<br />
PlasmidDNA Preparation<br />
RNAPreparation<br />
Genomic DNA Preparation<br />
Lysis/<strong>Protein</strong> Extraction<br />
Enzyme Regulation<br />
PlasmidDNA Purification<br />
Genomic DNA Purification<br />
High-abundance <strong>Protein</strong>Depletion<br />
<strong>Protein</strong> Enrichment<strong>and</strong> Immunoprecipitation<br />
Optional Steps<br />
PlasmidAmplification<br />
RNAPurification<br />
WholeGenomeAmplification<br />
Desalting/Buffer Exchange/Cleanup<br />
Concentration<br />
PCR<br />
RT-PCR<br />
PCR<br />
DNACleanup<br />
DNACleanup<br />
DNACleanup<br />
2-DElectrophoresis/DIGE<br />
Liquid Chromatography<br />
1-DElectrophoresis/Western Blotting<br />
Cloning&Sequencing<br />
Gene Expression<br />
Genotyping<br />
or<br />
Cloning&Sequencing<br />
Buffer Exchange/Cleanup<br />
Concentration<br />
Optional Steps<br />
Mass Spectrometry<br />
BiomoleculeofInterest: e.g., Biomarker<br />
Construction of<br />
expression-ready<br />
DNA clones<br />
Sample(s): cells, bacteria, yeast, etc.<br />
Overexpression <strong>and</strong> purification<br />
of specific protein(s),<br />
structure <strong>and</strong> function studies<br />
Optional Steps<br />
PlasmidDNA Preparation<br />
Lysis/<strong>Protein</strong> Extraction<br />
Enzyme Regulation<br />
1-DElectrophoresis<br />
Cloning&Sequencing<br />
<strong>Protein</strong>Expression<br />
<strong>Protein</strong>Capture <strong>and</strong>CleavageofTag<br />
Desalting/Buffer Exchange/Cleanup<br />
Concentration<br />
Liquid Chromatography<br />
<strong>Protein</strong>Interaction Analysis<br />
Desalting/Buffer Exchange/Cleanup<br />
Concentration<br />
Optional Steps<br />
Characterized Biomolecule: e.g.,Biomarker<br />
Mass Spectrometry<br />
imagination at work
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