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genomics For more information on these products contact your local VWR sales office, send an e-mail to vwrbiomarke@eu.vwr.com or visit our website www.vwr.com Versatile thermal cycler product range Thermo Scientific PCR instrument range includes two different thermal cyclers, Arktik and Piko, both providing excellent and reliable performance covering a broad range of applications from individual PCRs through to high throughput projects. Thermo Scientific Arktik thermal cyclers reliability and flexibility for everyday use in any laboratory Interchangeable heating blocks Arktik thermal cyclers offer three different interchangeable heating blocks: 96 well, 384 well and dual blocks with two 48 well units. The blocks can be changed in seconds without any additional tools. Dual 48 well blocks allow two users to run two independent protocols at the same time or create a new protocol while running the other. The blocks allow use of virtually all standard PCR plastics. High performance A broad range, up to 30 ºC, and accurate temperature gradient* is available for protocol optimisation and simplifies the user operation. Excellent thermal precision is a result of the unique heat sink design, as well as from the heated lid with over tightening protection system. Ease of use and convenience The Arktik thermal cycler features a highly intuitive user interface with clear protocol display for highly efficient programming and operation. In addition, the instrument has the benefit of a low noise level. Thermo Scientific Piko thermal cyclers high performance in a compact package Superior performance Although only half the size of other instruments, the Piko thermal cycler provides superior thermal performance, fast ramping rates and quick settling times, offering excellent PCR efficiency and well-to-well consistency. It enables the user to complete PCR protocols in less than 15 minutes. The automatic heated lid function ensures consistent and tight sealing from run-to-run. High thermal performance makes the Piko thermal cycler suitable for conventional and fast PCR applications. Two formats – 24 and 96 well blocks Available in 24 and 96 well formats, the Piko thermal cycler can use low profile PCR tubes, 8 tube strips as well as specialised 24 and 96 well Piko PCR plates. The 24 and 96 well Ultra- Thin Walled (UTW) Piko plates allow better temperature transfer and are only ¼ of the size of conventional microplates. Despite this they maintain industry standard well spacing and are compatible with multichannel pipettes and liquid handling instruments. Reduced consumption The unique design of the heating block and small plate format allow significant savings both in energy and plastics consumption so helping to reduce the environmental impact of your PCR. * Gradient feature not available in the U.S, Germany and Japan Description Cat. No. Arktik thermal cycler, base with gradient* 731-0231 Arktik thermal cycler, base without gradient 731-0232 Arktik thermal cycler, 96 well block 731-0233 Arktik thermal cycler, 48 well dual block 2 731-0234 Piko thermal cycler, 24 well system 731-0235 Piko thermal cycler, 96 well system 731-0236 See BioMarkeShop for special offer! VWR International I VWRbioMarke Issue 27 I September 2011 I 17
the market source for life science THE DELIVERY EXPERTS DNA and siRNA transfection with jetPRIME Transfection is a widely used technique for which numerous cationic lipid carriers have been developed over the last two decades. However, while improving efficiency over calcium phosphate, cationic lipids destabilise the cell membrane making the process toxic. Polyplus’ researchers have developed one of the first non lipidic transfection reagents, polyethylenimine (PEI) 1 which works through a “proton sponge” cell entry mechanism. This has been recognised as a major breakthrough in transfection techniques 2 . Polyplus-transfection continues product innovation offering jetPRIME, a powerful and versatile transfection reagent. jetPRIME is an entirely new reagent developed by Polyplus-transfection; it combines the best advantages of lipids, i.e. strong interaction with the nucleic acid cargo, with a natural cell entry pathway (endocytosis) followed by efficient proton sponge-mediated endosome escape (Figure 1). As a consequence of tight cargo binding, it can be used equally well for delivery of large plasmids or small siRNA and the combination of both. As a consequence of the cell entry mechanism, jetPRIME is efficient while remaining gentle to cells. Figure 1 – Transfection mechanism with jetPRIME . Plasmid DNA transfection A comparison of manufacturer’s transfection charts (Table 1) shows that jetPRIME requires only half the amount of plasmid and reagent used by competitor L2K. Even with lower amounts of reagent, the percentage of transfected cells is much higher with jetPRIME (70 - 90%) in all common cell lines tested (Figure 2). Moreover, an intact cell membrane and less transfection reagent in the cells leave the cells healthy with jetPRIME , as can be observed under a microscope (Figure 3). Figure 2. Transfection efficiency assessed by FACS analysis in various cell lines 24 hours following transfection in 24 well plates according to the manufacturer’s recommendation for competitor L2K and 0,5 µg plasmid, 1 µl reagent per well for jetPRIME . Figure 3. Phase contrast microscopy of HeLa cells 24 hours after transfections performed according to the manufacturer’s recommendations for each reagent. Many other cell lines of various origins, as well as primary cells, are transfected with unusually high percentages (Table 2, see also first customers’ results 3, 4, 5 ). Moreover, jetPRIME is recommended for virus production in classical media. siRNA transfection and cotransfection of DNA and siRNA Gene silencing using siRNA is somehow complementary to gene expression using plasmid DNA, and both approaches are being used widely to unravel complex biological mechanisms. This is why having a single multipurpose transfection reagent on the bench is convenient. jetPRIME leads to over 80% knock down of even highly expressed endogenous genes in a variety of cell lines 6 (Table 3) and can also be used for plasmid DNA and siRNA co-transfection experiments, with over 90% silencing using 10 nM siRNA (data not shown). 18 I VWR International I VWRbioMarke Issue 27 I September 2011
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