Aqueous and polar GPC/SEC columns

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PL aquagel-OH SEC columns Typical applications Heparin, gum, polyacrylic acid, polyacrylamide, pectin, dextran Conditions Columns: 3 x PL aquagel-OH MIXED 8 µm, 300 x 7.5 mm Eluent: 0.2 M NaNO 3, 0.01 M NaH 2PO 4, pH 7 Flow Rate: 1.0 mL/min Detector: PL-GPC 50 (RI) Figure 3. Polyvinyl alcohol Conditions Columns: 2 x PL aquagel-OH 30 8 µm, 300 x 7.5 mm Eluent: 0.2 M NaNO 3, 0.01M NaH 2PO 4, pH 7 Flow Rate: 1.0 mL/min Detector: PL-GPC 50 (RI) Figure 4. Heparin 6 Conditions Columns: PL aquagel-OH 60 15 µm, 300 x 7.5 mm PL aquagel-OH 40 15 µm, 300 x 7.5 mm Eluent: 0.2 M NaNO3, 0.01 M NaH2PO4, pH 7 Flow Rate: 1.0 mL/min Detector: PL-GPC 50 (RI) Figure 5. Hyaluronic acid Conditions Columns: 2 x PL aquagel-OH 20 5 µm, 300 x 7.5 mm Eluent: 0.25 M ammonium formate in water Flow Rate: 1.0 mL/min Inj. Vol: 20 µL Software: Agilent Cirrus GPC module for Agilent ChemStation CDS Detector: Agilent ELS (neb = 30 °C, evap = 30 °C, gas = 1.4 SLM) Figure 6. Differences in composition of two alkyl naphthalene sulfonates
PL aquagel-OH SEC columns Ordering information PL aquagel-OH Columns, 300 x 7.5 mm Description Particle size (µm) MW range (g/mol) (PEG/PEO) Guaranteed Efficiency (p/m) Part No. PL aquagel-OH 20 5 100 to 10,000 >55,000 PL1120-6520 PL aquagel-OH 30 8 100 to 30,000 >35,000 PL1120-6830 PL aquagel-OH 40 8 10,000 to 200,000 >35,000 PL1149-6840 PL aquagel-OH 40 15 10,000 to 200,000 >15,000 PL1149-6240 PL aquagel-OH 50 8 50,000 to 1,000,000 >35,000 PL1149-6850 PL aquagel-OH 50 15 50,000 to 1,000,000 >15,000 PL1149-6250 PL aquagel-OH 60 8 200,000 to >10,000,000 >35,000 PL1149-6860 PL aquagel-OH 60 15 200,000 to >10,000,000 >15,000 PL1149-6260 PL aquagel-OH MIXED-H 8 100 to 10,000,000 >35,000 PL1149-6800 PL aquagel-OH MIXED-M 8 >600,000 >35,000 PL1149-6801 Ordering information PL aquagel-OH Analytical Column accessories Description Quantity (pk) Part No. Frit Removal Tool for Threaded Columns only 1 PL1310-0001 Frit (2 µm) Kit for Threaded Columns, 7.5 mm ID Frit (5 µm) Kit for Threaded Columns, 7.5 mm ID 5 PL1310-0002 5 PL1310-0012 Column Connecting Nuts, 1/16 in. Tube 5 PL1310-0007 Tubing Ferrules, 1/16 in. Tube 5 PL1310-0008 LDV Intercolumn SS Connector 1 PL1310-0005 Connecting Tubing, 10 cm length, 0.01 in. ID 10 PL1310-0048 See also • Polymer Calibration Standards, with highly characterized molecular weights, publication 5990-7996EN 7 Ordering information PL aquagel-OH Guard Columns Description Particle size (µm) ID (mm) Length (mm) Part No. PL aquagel-OH Guard 10 25.0 25 PL1249-1120 PL aquagel-OH Guard 5 7.5 50 PL1149-1530 PL aquagel-OH Guard 8 7.5 50 PL1149-1840
Page 1 and 2: Aqueous and polar GPC/SEC columns P
Page 3 and 4: Agilent PL aquagel-OH SEC columns S
Page 5: PL aquagel-OH SEC columns High perf
Page 9 and 10: Agilent PolarGel GPC columns For in
Page 11 and 12: PolarGel-M For analyzing a wide ran
Page 13 and 14: Typical applications Sodium acrylat
Page 15 and 16: Recommendations for setting up a GP
Page 17 and 18: Typical polymer molecular weights I
Page 19 and 20: Agilent GPC/SEC Analysis Systems Fo

Aqueous and polar GPC/SEC columns

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