Advanced Flow Workshop Shumway Murphey

Workshop 2: Advanced Flow Workshop-
Wayne Shumway, CHT, CHS and
Cathi Murphey, MT(ASCP), CHS, BLM
W H E N I N L O G “M M O D E” E :
Log “Mode”: 1024 data points in log relation (Log Amp)
End of 1 st decade
End of 2 nd decade
End of 3 rd decade
End of 4 th decade
CHANNEL VALUES
256 512 768 1024
BD = “Log Data”
Coulter = “Linear Data”
1
RLV
200 400 600 800
10 100 1000
1000
10,000
10 0 10 1 10 2 10 3 10 4
200 400
600 800 1000
200 400 600 800 1000
Linear “Mode”: 1024 data points in linear relation
Log “Mode”: 1024 data points in log relation (Log Amp)
End of 1 st decade
End of 2 nd decade
End of 3 rd decade
End of 4 th decade
VALUES THAT WOULD HELP US BEST UNDERSTAND
THE QUANTITATIVE RELATIONSHIP BETWEEN THESE
TWO GROUPS OF PEOPLE?
256 512 768 1024
1
RLV
200 400 600 800
10 100 1000
1000
10,000
256 512
1 2 3 4 5 6 7 8 910
200 End of 1 st decade 400
Fluorescence Intensity
End of 2 nd decade
20 30 40 50 60 70 100
4
96 additional units
154 358 Channel Shift
100
512
10 0
RLV
Log “Mode”: 1024 data points in log relation (Log Amp)
End of 1 st decade
End of 2 nd decade
End of 3 rd decade
256 512 768 1024
200 400 600 800
10 1 10 2 10 3
Linear “Mode”: 1024 data points in linear relation
End of 4 th decade
1000
10 4
256 512 768 1024
CHANNELS USED FOR
“BINNING” DATA
Variations in light intensity identifiable by PMT ~infinite
Represented as a finite, linear range for practicality
Entire range = 1024 increments (Channels)
Linear Mode:
0 – 1.0 1.1 – 2.0 3.1 – 4.0
2.1 – 3.0
200 400 600 800 1000
Scales LOOK the same – BUT hidden log scale beneath in Log Mode!!
In Linear Mode the channels ARE “numbers”!!
Moving from the origin (0) to the end of the scale
(1024), each subsequent channel serves as a “bin”
for a higher range of values.
3