Stains & Reagents - by Hardy Diagnostics
Stains & Reagents - by Hardy Diagnostics
Stains & Reagents - by Hardy Diagnostics
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Acid-Fast TB <strong>Stains</strong><br />
The Acid-Fast Stain Kit is used as a differential method to detect those<br />
organisms which decolorize with treatment of acid alcohol, and those that do<br />
not, such as the mycobacteria.<br />
Kinyoun/Cold Method (Preferred)<br />
Specimen smear<br />
(heat or methanol<br />
fix)<br />
Carbol Fuchsin<br />
2-3 minutes<br />
required (no heat<br />
required).<br />
Water rinse<br />
Use Acid Alcohol<br />
Decolorizer until<br />
color is rinsed.<br />
Water rinse<br />
Acid Alcohol, 3% HCl in<br />
Alcohol<br />
For AFB staining, Kinyoun or Ziehl-<br />
Neelsen, <strong>by</strong> <strong>Hardy</strong> <strong>Diagnostics</strong>.<br />
250ml................................. AA008<br />
Acid-Fast Decolorizer,<br />
Sulfuric Acid 5%, in Alcohol<br />
<strong>by</strong> Medical Chemical.<br />
8 oz.................................. 3545A8<br />
AFB Stain Kit<br />
Carbol Fuchsin (Kinyoun), Acid<br />
Alcohol 3%, Methylene Blue<br />
Counterstain, <strong>by</strong> <strong>Hardy</strong> <strong>Diagnostics</strong>.<br />
3x8 oz................................. AF900<br />
Brilliant Green Counterstain<br />
For acid-fast stain procedures and<br />
other uses. 1% weight/volume. A<br />
saline solution of brilliant green.<br />
250ml................................. BG008<br />
1 gallon........................... 460A128<br />
AFB Brilliant Green Stain Kit<br />
Contains Carbol Fuchsin (Kinyoun),<br />
Acid Alcohol 3%, Brilliant Green<br />
Counterstain, <strong>by</strong> Medical Chemical.<br />
3x8 oz................................. 483KB<br />
A Culture of Service <br />
Counterstain,<br />
Methylene Blue or<br />
Brilliant Green<br />
Water rinse<br />
Carbol Fuchsin (Kinyoun)<br />
By <strong>Hardy</strong> <strong>Diagnostics</strong><br />
250ml................................. CF008<br />
16 oz................................. 483A16<br />
Methylene Blue Counterstain<br />
For acid-fast stain procedures and<br />
other uses. An aqueous solution<br />
of methylene blue, <strong>by</strong> Medical<br />
Chemical.<br />
16 oz................................. 675A16<br />
1 gallon............................675A128<br />
Acid-Fast Smear Fix<br />
For BACTEC blood cultures,<br />
for better slide adhesion<br />
of specimens, <strong>by</strong> <strong>Hardy</strong><br />
<strong>Diagnostics</strong>.<br />
15ml..........................................Z56<br />
<strong>Stains</strong> & <strong>Reagents</strong><br />
Q1
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