Renilla Luciferase Assay System Technical Manual, TM055

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E. References 1. Wood, K.V. et al. (1984) Synthesis of active firefly luciferase by in vitro translation of RNA obtained from adult lanterns. Biochem. Biophys. Res. Comm. 124, 592–6. 2. de Wet, J.R. et al. (1985) Cloning of firefly luciferase cDNA and the expression of active luciferase in Escherichia coli. Proc. Natl. Acad. Sci. USA 82, 7870–3. 3. Matthews, J.C., Hori, K. and Cormier, M.J. (1977) Purification and properties of Renilla reniformis luciferase. Biochemistry 16, 85–91. F. Related Products Synthetic Renilla Luciferase Reporter Vectors Product Size Cat.# phRL-null Vector (b,d,j) 20µg E6231 phRL-TK Vector (b,d,j) 20µg E6241 phRL-TK(Int–) Vector(b,d,j) 20µg E6251 phRL-SV40 Vector (b,d,j) 20µg E6261 phRL-CMV Vector (b,d,e,j) 20µg E6271 phRG-B Vector(b,d,j) 20µg E6281 phRG-TK Vector (b,d,j) 20µg E6291 Luciferase Reporter Vector Sequencing Primers Product Size Cat.# RVprimer3 (counter clockwise) 2µg E4481 RVprimer4 (clockwise) 2µg E4491 Luciferase Assay Systems Product Size Cat.# Dual-Luciferase® Reporter Assay System(c,f) 100 assays E1910 Dual-Luciferase ® Reporter Assay System 10 pack (c,f) 1,000 assays E1960 Dual-Luciferase ® Reporter 1000 Assay System (c,f) 1,000 assays E1980 Transfection Systems Product Size Cat.# TransFast Transfection Reagents (g) 1.2mg E2431 Transfectam® Reagent for the Transfection of Eukaryotic Cells(h) 1mg E1231 0.5mg E1232 Tfx-50 Reagent (i) 2.1mg E1811 Tfx-20 Reagent(i) 4.8mg E2391 Tfx-10 Reagent (i) 9.3mg E2381 Tfx Reagents Transfection Trio(i) 5.4mg E2400 ProFection ® Mammalian Transfection System - Calcium Phosphate 80 transfections E1200 ProFection ® Mammalian Transfection System - DEAE-Dextran 80 transfections E1210 Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · Telephone 608-274-4330 · Fax 608-277-2516 · www.promega.com Part# TM055 Page 12 Printed in USA. Revised 6/01
Luminometers Product Turner Designs Model TD-20/20 Luminometer Turner Designs Model TD-20/20 Luminometer with Printer Turner Designs Model TD-20/20 Luminometer with Single Auto Injector Turner Designs Model TD-20/20 Luminometer with Dual Auto Injector Turner Designs Model TD-20/20 Luminometer with Printer and Dual Auto Injector Cat.# E2041 E2051 E2351 E2361 E2061 (a) Patent Pending. (b) Certain applications of this product may require licenses from others. (c) U.S. Pat. No. 5,283,179, 5,641,641, 5,650,289, 5,814,471, Australian Pat. No. 649289 and European Pat. No. 0 553 234 have been issued to Promega Corporation for a firefly luciferase assay method, which affords greater light output with improved kinetics as compared to the conventional assay. Other patents are pending. (d) Licensed under U.S. Pat. Nos. 5,292,658, 5,418,155 and other patents. (e) The CMV promoter and its use are covered under U.S. Pat. Nos. 5,168,062 and 5,385,839 owned by the University of Iowa Research Foundation, Iowa City, Iowa, and licensed FOR RESEARCH USE ONLY. Commercial users must obtain a license to these patents directly from the University of Iowa Research Foundation. (f) U.S. Pat. No. 5,744,320 and Australian Pat. No. 721172 have been issued to Promega Corporation for quenching reagents and assays for enzyme-mediated luminescence. Other patents are pending. (g) The cationic lipid component of the TransFast Transfection Reagent is covered by U.S. Pat. Nos. 5,824,812, 5,869, 715 and pending foreign patents. (h) Transfectam is a registered trademark of BioSepra, S.A., the holder of a license from CNRS-ULP Strasbourg under U.S. Pat. No. 5,171,678 to sell the Transfectam ® product for research purposes only. The Transfectam ® product was developed by J.P. Behr and J.P. Loeffler and is covered by the aforementioned patent. (i) The cationic lipid component of the Tfx Reagents is covered by U.S. Pat. Nos. 5,527,928, 5,744,625 and 5,892,071, Australian Pat. No. 704189 and other pending foreign patents. (j) Organizations may use this product in their research and they may transfer derivatives of the product to colleagues at other Nonprofit Organizations provided that such colleagues agree in writing to be bound by the terms and conditions of this label license. Researchers may not transfer this product or its derivatives to researchers at other organizations, which are not Nonprofit Organizations, without the express written consent of Promega and without those entities having an express license from Promega for the use of the product. Other than as specifically provided here, an authorized transferee of this product shall have no right to transfer this product or its derivatives to any other person or entity. A Nonprofit Organization means (1) a university or other institution of higher education located in any country; (2) an organization of the type described in section 501(c)(3) of the Internal Revenue Code of 1954 [26 U.S.C. 501(c)(3)] and exempt from taxation under section 501(a) of the Internal Revenue Code [26 U.S.C. 501(a)]; or (3) any nonprofit organization located in a foreign country that would qualify as a nonprofit organization under paragraph (2) of this section if it were located in the United States. Any organization that is not "Nonprofit" as defined here is considered a for-profit organization. A Nonprofit Organization performing research for a for-profit organization also shall be considered for-profit. With respect to research use by for-profit organizations, or any diagnostic or therapeutic uses, please contact Promega Corporation for supply and licensing information. © 2001 Promega Corporation. All Rights Reserved. Dual-Luciferase and ProFection are trademarks of Promega Corporation and are registered with the U.S. Patent and Trademark Office. Tfx and TransFast are trademarks of Promega Corporation. Triton is a registered trademark of Union Carbide Chemicals and Plastics Co., Inc. All prices and specifications are subject to change without prior notice. Product claims are subject to change. Please contact Promega Technical Services or access the Promega online catalog for the most up-todate information on Promega products. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · Telephone 608-274-4330 · Fax 608-277-2516 · www.promega.com Printed in USA. Part# TM055 Revised 6/01 Page 13
Page 1 and 2: Renilla Luciferase Assay System INS
Page 3 and 4: II. Product Components Product Size
Page 5 and 6: luciferase is stable in cell lysate
Page 7 and 8: If using a luminometer fitted with
Page 9 and 10: B. Description of the New Synthetic
Page 11: 120 Relative Luminescence (%) 100 8

Renilla Luciferase Assay System Technical Manual, TM055

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