Development and Validation of an Automated ... - wise-rtd.info

Layman Report . Results . Effects on the environment
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buffer solution that is needed for the washing step is provided from a container
outside of the pipetting system. During the test, the pipetting needle is cleaned in the
wash station between every single pipetting step to avoid the displacing of reagents.
The absorption reader for the determination of the optical density at the end of the
procedure is integrated in the automat and forwards the measurement result to the
control software automatically. The integrated analysis software provides a detailed
report of the results. Due to the almost complete automation the instrument is easy to
operate.
3 Results
By means of highly sensitive immunoassays and standardised ELISA-Tests very
small limits of detection were received without the complex clean-up steps.
The statistical methods ensure reliable measurement values, since the determined
concentrations are specified together with e.g. the extended measurement
uncertainty.
Within the project, the prototype of the ESTR-A-LISER was employed successfully in
several validation and field studies for surface water and sewage treatment plants,
thereby limits of detection of 2 ng/L were obtained. In the routine operation between
10 and 20 samples per day can be analysed by the automat.
4 Effects on the environment
With the examples of 17β-Estradiol and 17α-Ethinylestradiol the successful
demonstration project showed that by means of ELISA-Tests with an adequate
standardisation and automation in combination with statistical methods small limits of
detections can be obtained validly. This technology permits the production of
inexpensive automats that easily can be adapted to further ELISA-Tests for the
determination of other substances.
Since by the EU – Environmental Framework Directive a multitude of substances is
classified as potentially hazardous to water, a measuring system that can be applied
quo data GmbH 10.11.2005