Available Online through - International Journal of Pharmacy and ...

Naga Pranitha Chodavarapu * et al. /International Journal Of Pharmacy&Technology
In vitro Disintegration Test (U.S.P.)
The U.S.P. device to test disintegration uses 6 glass tubes that are 3” long; open at the top and 10 mesh screen at
the bottom end. To test for disintegration time, one tablet was placed in each tube and the basket rack was positioned
in a 1-L beaker of 0.1 N Hydrochloric acid at 37 ± 20 C such that the tablet remain 2.5 cm below the surface of liquid
on their upward movement and not closer than 2.5 cm from the bottom of the beaker in their downward movement.
The basket containing the tablets was moved up and down through a distance of 5-6 cm at a frequency of 28 to 32
cycles per minute. Floating of the tablets can be prevented by placing perforated plastic discs on each tablet.
According to the test, the tablet must disintegrate and all particles must pass through the 10 mesh screen in the time
specified. If any residue remains, it must have a soft mass.
In Vitro Buoyancy Studies:
The in vitro buoyancy was determined by floating lag time, per the method described by Rosa et al. The tables
were placed in a 100-mL beaker containing 0.1N HCL. The time required for the tablet to rise to the surface and float
was determined as floating lag time. 16
In Vitro Dissolution Profile Studies:
The rate of metformin hydrochloride release from metformin hydrochloride sustained release tablets was
conducted using Dissolution Testing Apparatus II (Paddle method). The dissolution test was carried out using 900 ml
of 0.1 N HCI, at 37 ± 0.5 Celsius and 75 rpm. A sample (10 ml) of the solution was withdrawn from the dissolution
apparatus at every hour up to eight hours and withdrawn volume was replaced with fresh dissolution media. The
samples were filtered through a 0.45 µ membrane filter, the sample was diluted to suitable concentration with 0.1N
HCl. Absorbance of these solutions was measured at 233nm using UV spectrophotometer. 11
IJPT | June-2011 | Vol. 3 | Issue No.2 | 2725-2745 Page 2730