Northern Solutions DEPC Water Add 100ul DEPC per 100ml of ...
Northern Solutions DEPC Water Add 100ul DEPC per 100ml of ...
Northern Solutions DEPC Water Add 100ul DEPC per 100ml of ...
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20X SSC<br />
210.4gm NaCl<br />
105.8gm NaCitrate<br />
ddH2O water to about 1.1 Liters<br />
Adjust pH t0 7.0<br />
Bring up to 1.2L with ddH2O<br />
Aliquot into three 400ml bottles<br />
<strong>Add</strong> 400ul <strong>DEPC</strong> and let sit O/N<br />
Autoclave at least 25min<br />
0.05M NaOH<br />
1gm NaOH Pellets<br />
500ml <strong>DEPC</strong> water<br />
Low Stringency Wash Solution (2XSSC, 0.1%SDS)<br />
5ml 10%SDS<br />
50ml 20XSSC<br />
Bring up to 500ml with <strong>DEPC</strong> water<br />
High Stringency Wash Solution (0.1SSC, 0.1%SDS)<br />
5ml 10%SDS<br />
2.5ml 20XSSC<br />
Bring up to 500ml with <strong>DEPC</strong> water
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