Jana Tulinska - NanoImpactNet

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PHAGOCYTIC ACTIVITY AND RESPIRATORY BURST OF LEUKOCYTES The assay evaluates phagocytic activity and respiratory burst of polymorphonuclear neutrophils neutrophil / monocytes monocyte exposed to the test compound in vitro. Principle of the assay � Heparinised whole blood is incubated with the FITC-labelled Staphylococcus aureus bacteria and hydroxyethidine �� Overall verall percentage of neutrophils and monocytes showing phagocytosis in general (ingestion of one or more bacteria per cell) and oxidative burst activity was measured in triplicates for each variable
Phagocytic acitivity and respiratory burst of leukocytes Heparinized blood derived from humans Incubation on microplates with 3 concentrations of nanoparticles for 4h, 24h and 48h 30 µl of blood and 10 µl hydroethidium C-control, S-sample 15 minutes incubation at 37°C 3 µl of SPA particles are added to sample tubes 15 minutes incubation at 37°C Process of phagocytosis Samples are measured using flow cytometer 3 µl of SPA particles are added to Control tubes All tubes are put on ice. Add 700 µl of lysing solution
Page 1 and 2: The Effect of Two Iron Oxide Nanopa
Page 3 and 4: Aim is to develop testing strategie
Page 5 and 6: Nanoparticles for testing in biolog
Page 7 and 8: NP characterization Size distributi
Page 9 and 10: DESIGN OF EXPERIMENT • Blood dono
Page 11: Heparinized blood derived from huma
Page 15 and 16: Cytotoxic activity of natural kille
Page 17 and 18: Challenges Interference of method w

Jana Tulinska - NanoImpactNet

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