ABSTRACTS 2009 - Society for Invertebrate Pathology

ABSTRACTS
MONDAY MONDAY
Jong Yul Roh - School of Agricultural Biotechnology, Seoul National
University, Seoul, South Korea; Yeon Ho Je - School of Agricultural
Biotechnology, Seoul National University, Seoul, South Korea
Address for correspondence: jae-su.kim@uvm.edu
Applications of Beauveria bassiana SFB-205 (KCCM 10892P)
supernatant (10 ml L -1 ) mixed with polyoxyethylene-(3)-isotridecyl
ether (TDE-3) (CAS 24938-91-8) (0.1 ml L -1 ) as a spreader showed
90.1% and 64.3% control efficacy against Aphis gossypii Glover and
Myzus persicae Sulzer (Homoptera: Aphididae) adults, respectively, at two
days after the application in the greenhouse. Similarly, larger amounts
of protein were detected in the supernatant precipitates after incubation
with A. gossypii adults than M. persicae adults in the presence of TDE-
3 from SDS-PAGE. Peculiarly, the incubations with TDE-3 made the
bands stronger than those without TDE-3 in both aphid species.
Poster/Microbial Control - Monday, 16:30 - 17:30 MC-10
withdrawn
Poster/Microbial Control - Monday, 16:30 - 17:30 MC-11
The biofumigant effects of Muscodor albus on potato
tubermoth, Phthorimaea operculella, and codling moth, Cydia
pomonella.
Lerry Lacey - USDA, Agricultural Research Service, Wapato, WA, USA
Address for correspondence: lerry.lacey@ars.usda.gov
The endophytic fungus, Muscodor albus, produces several volatile
organic compounds (VOCs: alcohols, esters, ketones, acids and lipids)
that are biocidal for a range of organisms. We conducted research on
the insecticidal activity of M. albus VOCs on potato tubermoth (PTM)
(Phthorimaea operculella) and codling moth (CM) (Cydia pomonella).
The insecticidal activity of the fungus for control of PTM adults and
neonate larvae was demonstrated after 78 hours of exposure under
different temperature regimes and dosages of fungus. Adult PTM were
very susceptible (91% mortality) to 30 g of hydrated fungal mycelium on
rye seeds in a 28 liter chamber at 24°C. Neonate larvae under the same
conditions responded with 73% mortality. Three day-old larvae within
tubers were also susceptible but after longer exposures. A 7 day exposure
to VOCs produced 96% mortality. VOCs were also tested against CM
adults, neonate larvae, larvae in infested apples, and diapausing cocooned
larvae. Fumigation of adult CM with VOCs for 78 hours resulted in
81% mortality. Exposure of neonate larvae to VOCs for 78 hours on
apples and incubating for 7 days in fresh air resulted in 86% mortality.
Exposure of apples that had been infested for 5 days, fumigated with
VOCs for 78 hours, and incubated as above produced 71% mortality.
Diapausing cocooned CM larvae that were exposed to VOCs for 7 or
14 days resulted in 31 and 100% mortality, respectively. Treating several
stages of PTM and CM with VOCs indicate that M. albus could be an
alternative to broad spectrum chemical fumigants.
Poster/Microbial Control - Monday, 16:30 - 17:30 MC-12
Monitoring on resistance of brown planthopper (Nilaparvata
lugens) to imidacloprid and buprofezin
Jianhong Li - College of Plant Science and Technology, Wuhan, Hubei
Province, People’s Replublic of China; Liang Yao - College of Plant
Science and Technology, Wuhan, Hubei Province, People’s Replublic
of China; Chunhua Qin - College of Plant Science and Technology,
Wuhan, Hubei Province, People’s Replublic of China; Wei Xu - General
Station of Hubei Plant Protection, China, Wuhan, Hubei Province,
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People’s Replublic of China; Kaixiong Zhang - General Station of
Hubei Plant Protection, China, Wuhan, Hubei Province, People’s
Replublic of China; Chuanhua Peng - General Station of Hubei Plan
Protection, China; Hangang Luo - General Station of Hubei Plan
Protection, China,
Address for correspondence: jianhl@mail.hzau.edu.cn
In 2007, Seven field populations of Nilaparvata lugens were collected
from Tongcheng, Wuxue, Tianmen, Jianli, Gongan, Xiaogan and
Zaoyang respectively. The toxicity assay of imidacloprid and buprofezin
to seven populations were done by rice stem-dipping method. The results
showed that Tongcheng, Wuxue, Tianmen, Jianli, Gongan, Xiaogan and
Zaoyang field populations produced high-level resistance to imidacloprid
with resistance index varied from 69.00 to 153.33 compared with the
susceptible colony. Xiaogan field population had high resistance to
buprofezin with resistance index of 41.06, Tongcheng, Wuxue, Gongan,
Jianli, Tianmen and Zaoyang field populations had middle-level resistance
to buprofezin with resistance index varied from 13.39 to 23.40.
Poster/Microbial Control - Monday, 16:30 - 17:30 MC-13
Selection of Chilean entomopathogenic fungal strains to
yellowjacket wasps.
Loreto Merino - INIA Quilamapu, Chillán, Bio Bio, Chile; Andrés
France - INIA Quilamapu, Chillán, Bio Bio, Chile; Marcos Gerding -
INIA Quilamapu, Chillán, Bio Bio, Chile
Address for correspondence: lmerino@inia.cl
The yellowjacket wasp, Vespula germanica, is considered a serious pest of
productive and recreational activities worldwide. A pathogenicity study
was carried out with a Chilean collection of 29 isolates of Metarhizium
anisopliae and 30 of Beauveria bassiana against worker, male and queen
wasps. Wasps of same age were fed with liquid sugar baits containing
1x108 conidia mL-1 suspensions of each isolate. Mortality was evaluated
daily and the sporulation on the cadavers. The highest mortality and
sporulation (p= 0.015) were obtained with isolates Qu-B941 and Qu-
B933 of Beauveria bassiana, reaching 79 and 95% for workers, 66 and
73% for males and 63 and 81% for queens, respectively. A second
experiment was accomplished with the isolates that reached percentile
90 of mortality and sporulation. Two isolates were provided at different
concentration (0 to 1x108 conidia mL-1) by liquid bait to workers, then,
mortality was evaluated daily. The results showed that 1 x 108 conidia
mL-1 increased up to 90 and 97% workers mortality with Qu-B941 and
Qu-B933 isolates, respectively.
Poster/Microbial Control - Monday, 16:30 - 17:30 MC-14
Conidial mass production of entomopathogenic fungi and
tolerance of mass-produced conidia to UV-B radiation and heat.
Drauzio E. N. Rangel - Utah State University, Logan, UT, USA;
Éverton K. K. Fernandes - Utah State University, Logan, UT, USA;
Helen G. Bignayan - Bureau of Plant Industry, Philipinnes, Jordan,
Guimaras, Philippines; Hernani G. Golez - Bureau of Plant Industry,
Philipinnes, Jordan, Guimaras, Philippines; Donald W. Roberts - Utah
State University, Logan, UT, USA
Address for correspondence: drauzio@biology.usu.edu
The most common systems worldwide for conidial production utilize
sterile rice grains as the growth substrate. The current study investigated
conidial mass production of six species of entomopathogenic fungi
[Metarhizium anisopliae (Ma), Beauveria bassiana (Bb), Aphanocladium
album (Aa), Lecanicillium aphanocladii (La), Simplicillium lanosoniveum
(Sl) and Verticillium lecanii (Vl)] on white basmati rice or premium