IACUC Forms

IACUC eNewsletter Issue No 2, 2007
2. Saphenous vein:
A technique for obtaining blood from the saphenous vein of mice and other small animals has
been described and is rapidly becoming the technique of choice for many investigators.
This procedure does not require that the mouse be anesthetized to collect a blood sample,
and is much less invasive than retro-orbital method. A tube can be used to restrain the
mouse, and the hind leg is extended by applying gentle downward pressure just above the
knee. The hair over the tarsal area is shaved with clippers followed by a number 11 scalpel
blade, and the vein is pricked with a needle (25 gauge is usually adequate). Blood can be
collected in a microcapillary tube.
Smearing a small amount of silicone grease over the area to be punctured helps to prevent
the blood from coming into contact with the skin and minimizes blood clotting.
When the blood has been collected, gentle pressure applied with a piece of gauze should be
used to effect hemostasis. Pictures demonstrating this technique may be found at the web
site: http://www.uib.no/vivariet/mou_blood/Blood_coll_mice_.html
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An update on the use of Buprenorphine
(Source: from the “Vet Regulatory News” June 2007).
In Aug 06, AVA received notification from MOH that Buprenorphine or B has been classified
as a Class A Controlled Drug under the First Schedule of the Misuse of Drugs Act w.e.f. 14
Aug 06. The sole supplier, Schering-Plough can no longer import B which effectively bans the
use of B for vet use.
MOH received an appeal from a vet for MOH to allow the use of B as an analgesic for
veterinary practice. On 16 Mar 07, AVA sought feedback from practicing vets on MOH’s
position and requested vets to indicate if they are required to use B in their course of work.
AVA has sent the consolidated feedback and appeal for MOH to re-consider permitting vets to
use B as an effective analgesia for alleviation of pain in animals.
In May 2007, MOH replied to indicate that AVA should discuss the matter with the Central
Narcotics Bureau (CNB) as the use of B falls under the Misuse of Drugs Act.
The AVA is currently in talks with CNB and would update the veterinary community
accordingly.
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