Instant Blue Two-Color SDS Marker - Vwr-cmd.com

Electrophoresis
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>ClearPage
Instant Blue
stain instructions
BEFORE USE: Mix the Instant Blue solution immediately before use by
gently inverting the bottle a few times (do not shake the bottle to mix
the solution). One may rinse the gel surfaces briefly with ultrapure water,
but do NOT wash the gel, as standing in water before staining will
reduce band sharpness. Fixing is also NOT recommended.
GEL STAINING: Place each gel in a separate small container (lids for
yellow pipette tips work well). Use the following amount of stain for
different size gels:
• Use 25 ml per gel for gels approximately 8 x 8 cm (from 10 x 10 cm
cassettes, such as ClearPage or Invitrogen gels)
• Use 20 ml per gel for gels approximately 6 x 8 cm (from 8 x 10 cm
cassettes, such as BIO-RAD, precise or gradipore gels)
Larger volumes are required for larger gels and for larger containers.
Shake gently until desired band intensity is achieved. Generally, 50 ng or
less can be seen in 10 minutes. Band intensity reaches a maximum in 30
to 60 minutes depending on gel thickness. Destaining does NOT improve
sensitivity, but the gels may be washed in ultrapure water for 15 minutes
to remove the free stain from the gel and get clear backgrounds. The gel
may be stored in ultrapure water.
GEL DRYING: The ClearPage Composite-polymer gels may be dried
without cracking between cellophane after equilibrating with ClearPage
gel drying solution (731-2052) or with 5% glycerol and water (25 ml per
gel, rock for 15 minutes); no alcohol is required. For other gels, follow
the manufacturer’s recommendations for solutions before drying the gel,
but leave the gel in solutions containing alcohol or other solvents for no
more than 5 minutes, or stain intensity will diminish.
Instant blue for… pack Cat. No.
Enough for 40-50 mini-gels 1 l 731-2058
Enough for 4-5 mini-gels 100 ml 731-2060
Trial size 25 ml 731-2059
Description
Cat. No.
Two-Color SDS Marker, 30 µl 731-2061
Two-Color SDS Marker, 0,6 ml 731-2062
VWR International Issue 18 September 2007 47