Excel

C18
Mechanisms of
Separation
Hydrophobic binding
interactions
Shape selectivity
Target Analytes
Analytes differing in hydrophobicity
Polar, moderately polar and nonpolar analytes
Uncharged acids and bases
Ionized acids or bases using ion-pairing
Recommended Applications
Analytes differing in hydrophobicity
Strength of
Interaction
Strong
Weak
Homologous compounds differing by –CH2
Ideal starting point for method development
Further Information;
An HPLC product brochure discussing ACE C18 and all
ACE phases is available.
Contact your distributor to request your copy or visit
www.ace-hplc.com for further details.
6
Figure 6: Comparison of selectivity and characteristics of
ACE ® Excel TM
2µm C18 to other manufacturers’ columns
Application #1503 – Rapid UHPLC Screening of 16 Pharmaceuticals and Related Compounds
Mobile Phase: A = 20 mM KH 2 PO 4 , pH 2.7 and
B = 20 mM KH 2 PO 4 , pH 2.7 in MeOH/H 2 O (65:35 v/v)
Gradient: 3 to 100% B in 5 minutes
Flow Rate: 0.6 ml/min
Temperature: 60°C
Detection: UV, 214 nm
Column Dimensions: 50 x 2.1mm
ACE Excel 2 C18
ZORBAX Eclipse
1.8 XDB C18
Waters ACQUITY
1.7 BEH C18
Phenomenex
Kinetex 1.7 C18
0
1
2
1
1,2
1
1. N-acetylprocainamide
2. 3-hydroxybenzoic acid
3. pindolol
4. methylphenylsulphoxide
5. benzylalcohol
6. quinoxaline
7. 1,4-dinitrobenzene
8. phenacetin
Comparative data may not be representative of all applications.
Please see p.10 for acknowledgement of trademarks
2
1
3,4
2
3
4
5
3
5
3
4
5
4
5
6
2
6
7
6
6
7
Minutes
9. 1,2-dimethoxybenzene
10. furosemide
11. anisole
12. methylbenzoate
13. remacemide
14. nimesulide
15. ethylbenzoate
16. diflunisal
In this example the ACE Excel C18 UHPLC column provides retention
and selectivity similar to the other C18 UHPLC columns (although
there may be slight selectivity differences between different C18
phases). Under these conditions, these slight differences allow the ACE
Excel C18 to fully separate all 16 components of interest.
As with all ACE HPLC columns, ACE Excel UHPLC columns also deliver
excellent peak shape for your analytes. Additionally, owing to the
optimal 2µm particle size and a rigorous classification protocol, back
pressure for all ACE Excel UHPLC columns is significantly lower than
for these leading UHPLC columns packed with