Preparation of a standard Melamine solution
Preparation of a standard Melamine solution
Preparation of a standard Melamine solution
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12<br />
Regarding the <strong>Melamine</strong> Crisis<br />
<strong>Preparation</strong> <strong>of</strong> a <strong>standard</strong> <strong>Melamine</strong> <strong>solution</strong><br />
Background information <strong>of</strong> <strong>Melamine</strong> (2, 4, 6-triamine-s-triazine)<br />
It is only slightly soluble in water.<br />
It is soluble to warm (hot) water and diluted hydrochloric acid.<br />
However, it is insoluble in hydrophobic solvents.<br />
<strong>Preparation</strong> <strong>of</strong> <strong>standard</strong> stock <strong>solution</strong> <strong>of</strong> <strong>Melamine</strong><br />
1) Measure 50mg <strong>of</strong> <strong>Melamine</strong>.<br />
2) Dissolve it by 20% Methanol <strong>solution</strong> and making it at 50mL.<br />
3) Use this <strong>solution</strong> as a 1,000ppm <strong>standard</strong> stock <strong>solution</strong>.<br />
<strong>Preparation</strong> <strong>of</strong> <strong>standard</strong> <strong>Melamine</strong> <strong>solution</strong><br />
1) Whenever using the above <strong>standard</strong> stock <strong>solution</strong>, always add 1% trichloroacetic acid and dilute it.<br />
2) Then use the <strong>solution</strong> as a <strong>standard</strong> <strong>Melamine</strong> <strong>solution</strong>.<br />
For example:<br />
1) Take 1mL <strong>of</strong> the <strong>standard</strong> stock <strong>solution</strong> and add 1% trichloroacetic acid and make it at 10mL.<br />
2) This 10mL will be the <strong>standard</strong> <strong>Melamine</strong> <strong>solution</strong>.<br />
3) In this case, 0.1mg <strong>of</strong> <strong>Melamine</strong> will be contained in a 1mL <strong>of</strong> <strong>standard</strong> <strong>Melamine</strong> <strong>solution</strong>.