MiPsummer Programme pdf - Mitochondrial Physiology Society

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Abstract # 3
Aerobic interval training reverses differences in mitochondrial function between rats inbred
for low- and high aerobic capacity
M. Wohlwend, T. Stølen, M. Høydal, U. Wisløff, and Ø. Rognmo.
Department of Circulation and Medical Imaging, St. Olav Hospital, Norwegian University of
Science and Technology, Trondheim, Norway.
Background Statistical association between low aerobic capacity and all-cause morbidity and
mortality has been shown 1 . Moreover, inbreeding rats for low- and high intrinsic aerobic capacity
was linked to manifestation of cardiovascular risk factors 2 . These findings point towards an
impaired oxygen metabolism segregating healthy function from dysfunction. However,
mechanistically association between low oxygen metabolism and low health remains unresolved.
Objectives Mitochondrial oxidative phosphorylation (OXPHOS) capacity was measured in 22
female rats artificially inbred for low- and high aerobic capacity (LCR; HCR, respectively) for 30
generations. The rats were randomized to either sedate or aerobic interval training (AIT) sessions 5
times a week for 1 month and then 2 times a week for 8 months.
Methods Mitochondrial OXPHOS capacity was assessed using high-resolution respirometry in
permeabilized muscle fibers of gastrocnemius (GASTRO) and left ventricle (LV) of the heart. The
measurements were blinded and corrected for wet-weight, but not yet adjusted for mitochondrial
content.
Results Baseline results for GASTRO in sedate LCR compared to sedate HCR showed reduced
respiration rates for fatty acid (p0.01), complex I (p