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Practical Aspects: The Conduct of TDI Experiments• IC 50shift experiment:Another abbreviatedexperimental design toidentify TDI• Run as a typical IC 50experiment in the ‘control’state• Compared to an IC 50determined after the testcompound has beenpreincubated with enzymeand NADPH for 30 min• If IC 50difference is 1.5X ormore, the compound is aninactivator% of control activity10075502500.01 0.1 1 10 100[inactivator] (uM)inactivation incubation without NADPHinactivation incubation with NADPH23