Biological Safety Manual - University of Florida

Table of ContentsAuthority & Responsibilities ............................................................................................. 6Policy Statement ............................................................................................................. 6Responsibilities ............................................................................................................... 7Biosafety: Fundamentals and Definitions ........................................................................ 7Biohazards ................................................................................................................................................. 8Regulatory Authority .................................................................................................................................. 8Biohazards Risk Assessment .......................................................................................... 9Objective .................................................................................................................................................... 9Risk Assessments ..................................................................................................................................... 9Human Pathogens ................................................................................................................................... 10Animal Pathogens ................................................................................................................................... 12Plant Pathogens and Greenhouses ........................................................................................................ 14Select Agents .......................................................................................................................................... 15Recombinant and Synthetic Nucleic Acids .............................................................................................. 16Biological Toxins ...................................................................................................................................... 18Mammalian Cell Cultures ........................................................................................................................ 19Human Blood and Other Potentially Infectious Material .......................................................................... 19Clinical Laboratories ................................................................................................................................ 20Viral Vectors ............................................................................................................................................ 21Biosecurity ..................................................................................................................... 29Control of Biohazards .................................................................................................... 32Laboratory Practices ................................................................................................................................ 32Engineering Controls ............................................................................................................................... 33Personal Protective Equipment ............................................................................................................... 36Administrative Controls ............................................................................................................................ 37Decontamination and Sterilization of Biologicals ........................................................... 39Disinfectants ............................................................................................................................................ 40Biomedical and Biological Waste ............................................................................................................ 42Animal Carcasses .................................................................................................................................... 44Disposal of Biological Toxins ................................................................................................................... 45Autoclave Testing and Use...................................................................................................................... 45Equipment Decontamination ................................................................................................................... 46Laboratory Decontamination ................................................................................................................... 47Emergencies ................................................................................................................. 47Personnel Responsibilities/Assignments During a Disaster .................................................................... 47Emergency Evacuation Procedures ........................................................................................................ 48Fire Safety ............................................................................................................................................... 49Break-In/Security Breach ......................................................................................................................... 49Workplace Violence ................................................................................................................................. 503