Fluorescent Labels and Dyes - ATTO-TEC GmbH

Fluorescent Labels
ATTO 633
Features:
absorbance
•
•
•
•
•
•
λabs = 629 nm
εmax = 1.3 x 105 M-1 cm-1 λfl = 657 nm
High fl uorescence yield
High thermal and photostability
Moderately hydrophilic
Good solubility in all polar solvents
Stable at pH 4 - 11
Cationic dye, perchlorate salt
Cy5
0 10 20 30 40 50 60
time of irradiation, min
Modifi cation MW, g/mol M + , g/mol Unit Order Code
with free COOH 652.2 552
NHS-ester 749.3 649
maleimide 774.3 674
biotin derivative 962.7 862
300 400 500 600 700 800 900 1000
wavelength, nm
1 mg
5 mg
1 mg
5 mg
1 mg
5 mg
1 mg
5 mg
AD 633-21
AD 633-25
AD 633-31
AD 633-35
AD 633-41
AD 633-45
AD 633-71
AD 633-75
Other conjugates with, e.g. streptavidin, sec. antibodies, phalloidin etc. on request.
absorbance
Solvent: Water
ηfl = 64 % CF = 0.05
260
τfl = 3.2 ns CF = 0.06
280
ATTO 633
fluorescence
Modifi cation MW, g/mol M + , g/mol Unit Order Code
with free COOH 531.1 431
NHS-ester 628.1 528
maleimide 653.2 553
36 37
absorbance
λabs = 635 nm
εmax = 1.2 x 105 M-1 cm-1 λfl = 659 nm
300 400 500 600 700 800 900 1000
wavelength, nm
biotin derivative 841.5 741
Fluorescent Labels
Solvent: Water
ηfl = 25 % CF = 0.13
260
τfl = 1.9 ns CF = 0.10
280
Features:
•
•
•
•
•
•
fluorescence
-
ClO4 1 mg
5 mg
1 mg
5 mg
1 mg
5 mg
1 mg
5 mg
ATTO 635
High fl uorescence yield
High photostability
Moderately hydrophilic
Good solubility in all polar solvents
Stable at pH 2 - 8
Cationic dye belonging to new class of carbopyronins, perchlorate salt
AD 635-21
AD 635-25
AD 635-31
AD 635-35
AD 635-41
AD 635-45
AD 635-71
AD 635-75
Other conjugates with, e.g. streptavidin, sec. antibodies, phalloidin etc. on request.
N
+
N
COOH