Protocol for EZ-Tn5™ pMOD™ Transposon ...
Protocol for EZ-Tn5™ pMOD™ Transposon ...
Protocol for EZ-Tn5™ pMOD™ Transposon ...
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EPICENTRE<strong>EZ</strong>-Tn5 pMOD<strong>Transposon</strong> Construction VectorsFigure 4. pMOD-3 <strong>Transposon</strong> Construction Vector.Xmn I 2417Sca I 2298BsrF I 1898Ahd I 1818EcoO109 I 2794Aat II 2740 1Ssp I 2622Amp R<strong>EZ</strong>-Tn5pMOD-3<strong>Transposon</strong>ConstructionVector2805 bpColE1oriNde I 184Nar I 236 Pvu II / PshA I 308PCRFPEco R I 374MEMCSR6KγoriMEHind III 425Pvu II / PshA I 749PCRRPAfl III 925PCRFP248-266AlwN I 1341SqRPSqFP347-373691-714R6KγoriME MCS MEPCRRP805-827Apo IEcoR I250 350 450 550 650 850Pvu IIPshA IBan IISac IEcoR IKpn IHind IIIAva ISma IXma IBamH IXba IAcc IHinc IISal IPst IPvu IIPshA ISph IHind IIIGAATTCGAGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTT380 390 400410420430SqFP = pMOD Foward Sequencing PrimerSqRP = pMOD Reverse Sequencing PrimerPCRFP = pMOD Forward PCR PrimerPCRRP = pMOD Reverse PCR PrimerME = Mosaic EndMCS = Multiple Cloning Site5' GCCAACGACTACGCACTAGCCAAC 3'5' GAGCCAATATGCGAGAACACCCGAGAA 3'5' ATTCAGGCTGCGCAACTGT 3'5' GTCAGTGAGCGAGGAAGCGGAAG 3'5' AGATGTGTATAAGAGACAG 3'page 8
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