Thomas Julou, Nicolas Desprat, Vincent Croquette, David Bensimon

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Experimental designnutrient + air๏ an automated continuousculture: a chemostat55°C๏ a gradient of selectivepressure:a temperature gradient35°CwasteJournées du LPS - 17 juin 20089

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Requirements๏ Our aim: focus on the effect of ecologicalinteractions (competition) in adaptation.• Structured environment• Long timescale• Contamination-proofautomated continuousculture devicei.e. chemostatJournées du LPS - 17 juin 20088

Experimental designnutrient + air๏ an automated continuousculture: a chemostat55°C๏ a gradient of selectivepressure:a temperature gradient35°CwasteJournées du LPS - 17 juin 20089

Page 1 and 2: Evolution, competition,cooperation
Page 3 and 4: “Adaptive evolution”definition:
Page 6 and 7: Experimental evolution milestones
Page 10 and 11: Experimental design∂E∂t = c E.
Page 12 and 13: Monitoring adaptation๏ Real-time
Page 14 and 15: An alive “game of life”๏ Our
Page 16: Cooperation at the single cell leve

Thomas Julou, Nicolas Desprat, Vincent Croquette, David Bensimon

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