Diversity of non-reducing polyketide synthase genes - Real JardÃn ...

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Table 1 (continued)Organism Source Major secondarysubstances foundin samplePhylogeneticgroupDNA ## of PKSsfoundGB accessionnumber andclone numberPertusaria scaberula Australia, Archer (ESS20867) lich, tham VA 327 1 AY918787 (0327C)Pertusaria scaberula Australia, Archer P932 (NSW) lich, tham VA 1448 2 AY918788 (1448B)AY918789 (1448C)Pertusaria subventosa Australia, Elix (ESS 15602) lich, tham, pic VA 385 1 AY918790 (0385B)Pertusaria subventosa Australia, Lumbsch 19070a (F) lich, tham, pic VA 1078 2 AY918791 (1078A)AY918792 (1078G)Pertusaria subverrucosa New Zealand, Wright 7560 (HBWright)norst PS 1067 2 AY918793 (1067A)AY918794 (1067C)Pertusaria subverrucosa New Zealand, Wright 7560 (HB norst PS 1068 1 AY918795 (1068X)Wright)Pertusaria velata Australia, Archer (ESS 21500) lec, lich VC 632 1 AY918796 (0632X)Pertusaria xanthoplaca Austalia, Lumbsch 19070b (F) 4,5 dichl PS 1079 1 AY918797 (1079A)Varicellaria rhodocarpa Austria, Soukup & Türk 29416(HB Türk)lec VC 528 2 AY918798 (0528A)AY918799 (0528B)Herbarium acronyms follow Holmgren et al. (1990).Abbreviations for secondary substances: const = constictic acid; cryptost = cryptostictic acid; fum = fumarprotocetraric acid; gyr = gyrophoric acid; lec = lecanoric acid; lich = lichexanthone;norst = norstictic acid; pic = picrolichenic acid; protocet = protocetraric acid; sal = salazinic acid; st = stictic acid; suc = succinprotocetraric; tham = thamnolic acid; thio = thiophaninic acid;var = variolaric acid; 2 0 -O-met=2 0 -O-methylperlatolic acid; 4,5-dichl = 4,5-dichlorlichexanthone. Abbreviations for phylogenetic groups: PS = Pertusaria s.str., VC = ’’Varicellaria’’-group,VA = ’’Variolaria ’’-group, O = Ochrolechia, C=Coccotrema.1246 I. Schmitt et al. / Phytochemistry 66 (2005) 1241–1253
I. Schmitt et al. / Phytochemistry 66 (2005) 1241–1253 1247Fig. 2. Phylogeny of non-reducing KS sequences from lichenized and non-lichenized fungi. Branches in bold print indicate significant statisticalsupport (posterior probabilities >0.94). Sequences retrieved from GenBank are indicated by GB accession numbers. Sequences generated for thecurrent study are labeled with species name, DNA extraction number (see Table 1), and name of clone (capital letter). Secondary metabolites ofcharacterized PKSs are typed in bold print behind the respective taxa. Groupings of Pertusarialean PKS genes that correspond to phylogenetic cladesfound in rDNA phylogenies are bracketed and named. (a) Upper part of phylogenetic tree, (b) lower part.Gibberella sp. (AY495593, AJ414140) and Magnaporthegrisea (AACU01001420) with unknown functions, andGibberella fujikuroi PKS4 (AJ278141), which encodes aan enzyme for the biosynthesis of the red polyketide pigmentbikaverin (Linnemannstöns et al., 2002). The closerelationship of PKSs involved in the synthesis of mycotoxinsand pigments has been observed before (Grazianiet al., 2004).Clade II contains four Pertusarialean fungi from thePertusaria s.str.-group (P. pertusa, P. kalelae, P. gibberosaand P. lecanina), which all produce chlorinated xanthones,plus uncharacterized PKSs of A. terreus,
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